Grouping of rice mutant lines based on morphological and agronomical traits under different moisture conditions using multivariate statistical methods

Document Type : Research Paper

Authors

1 Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran.

2 Department of Plant Production, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran.

3 Nuclear Agriculture Group, Nuclear Science and Technology Research Institute, Karaj, Iran.

Abstract

To study the morphological and agronomical traits and determine heterotic patterns at the reproductive stage of 96 mutant rice genotypes (M2), two separate experiments were carried out under drought stress and flooding conditions at Gonbad Kavous University, Iran, in 2016 using a randomized complete block design with three replications. A factor analysis was conducted to reduce the number of variables to fewer independent factors, and three and four factors were identified under the flooding and drought conditions, explaining 68.3% and 76.05% of the total variance, respectively. Cluster analysis using the selected factors by the Ward’s minimum variance method and Euclidean distance led to grouping of the mutant rice genotypes. The best cutting points of the dendrograms were determined by the discriminant function analysis and four clusters were identified at both irrigation conditions, which were also significantly different. Genotypes of the first and second clusters had the highest yield and its components under the flooding condition. In contrast, under the drought condition, genotypes of the third and fourth clusters were higher yielding and more tolerant to the drought stress. Mutants No. 4, 18, 25, 28, 29, 43, 44, 48, 53, 72, 79, 88, 90, 91, 94 and 95 were present in the first and second clusters of the flooding condition, and in the third and fourth clusters of the drought-stress condition, indicating their superiority over other mutants in terms of grain yield and drought tolerance. The results obtained from this study can be used for selecting suitable parents for hybridization from different clusters to produce new rice cultivars.

Keywords

Article Title [Persian]

گ گروه‌بندی لاین‌های موتانت برنج از نظر صفات مهم مورفولوژیک و زراعی در شرایط متفاوت رطوبتی با استفاده از تجزیه های آماری چند متغیره

Abstract [Persian]

به­ منظور مطالعه صفات مورفولوژیک و زراعی و تعیین الگوی هتروتیک در مرحله زایشی 96 ژنوتیپ موتانت برنج  (M2)، دو آزمایش جداگانه تحت شرایط تنش خشکی و غرقاب در قالب طرح بلوک­ های کامل تصادفی با سه تکرار در دانشگاه گنبد کاووس در سال 1395 اجرا گردید. تجزیه به عامل­ ها به منظور کاهش تعداد متغیرها به تعداد کمتری از عامل­ های مستقل از هم منجر به شناسایی سه و چهار عامل، به ترتیب در شرایط غرقاب و خشکی، شد که  86/3 و 76/5 درصد واریانس کل را توجیه کردند. تجزیه خوشه­ ای با استفاده از عامل­ های انتخابی به روش Ward و فاصله اقلیدوسی منجر به گروه­ بندی ژنوتیپ­ های موتانت برنج شد. به منظور تعیین بهترین نقطه برش دندروگرام­ ها از تجزیه تابع تشخیص استفاده شد که نتیجه آن تولید چهار خوشه با تفاوت معنی ­دار از هم در هر دو شرایط آبیاری شد. در شرایط غرقاب ژنوتیپ­ های خوشه ­های اول و دوم بیشترین عملکرد و اجزای عملکرد را داشتند. از طرفی، در شرایط خشکی ژنوتیپ ­ های خوشه­ های سوم و چهارم پرمحصول­ تر و متحمل ­تر به تنش خشکی بودند. موتانت ‌های شماره 4، 18، 25، 28، 29، 43، 44، 48، 53، 72، 79، 88، 90، 91، 94 و 95 در شرایط غرقاب در خوشه‌ های اول و دوم و در شرایط تنش خشکی در خوشه ­های متحمل سوم و چهارم قرار گرفتند و بنابراین از نظر عملکرد دانه و تحمل به تنش خشکی به سایر موتانت­ ها برتری داشتند. از نتایج حاصل از این پژوهش می­ توان برای انتخاب هدفمند والدین مناسب از خوشه­ های متفاوت به‌منظور دورگ­ گیری و تولید رقم ­های جدید برنج استفاده نمود.

Keywords [Persian]

  • برنج
  • تجزیه به عامل ها
  • تجزیه تابع تشخیص
  • تجزیه خوشه ای
  • خشکی

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Journal of Plant Physiology and Breeding
Volume 9, Issue 2
December 2019
Pages 85-96

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