Impact of biological and chemical treatments on the improvement of salt tolerance in wheat

Document Type : Research Paper


1 Former MSc Student of Soil Science, Department of Soil Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

2 Department of Soil Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

3 Department of Agronomy and Plant Breeding, Shahid Chamran University of Ahvaz, Ahvaz, Iran.


Salinity stress has been known as an important constraint limiting agricultural production especially in arid and semi-arid regions. Among several strategies to improve crop growth under salt stress, using of salinity tolerant Trichoderma isolates and silicon application could be an effective and easily adaptive strategy. In order to evaluate silicon and Trichoderma virens inoculation effects on some physiological and morphological properties of wheat grown under saline condition, a greenhouse experiment arranged as factorial based on completely randomized design with three replications was carried out. The factors included three levels of salinity (E1:3, E2:7 and E3: 10 dS m−1) from NaCl, CaCl2 and MgCl2 sources (3:2:1 ratio, respectively), two levels of Si, 0 (S1) and 1.5 mM (S2), from the source of Na2SiO3 and two levels of Trichoderma virens (with and without inoculation). It was shown that salt stress caused very significant reduction in plant height, chlorophyll content, grain yield and other measured properties. Salinity stress increased proline and soluble sugar concentration, Na/K and Na/Ca ratios in leaves. Application of Si to the growth medium significantly increased chlorophyll content, grain yield of wheat grown under normal as well as under saline environments, but those influences were lower than the fungus effect. These results seem to show that silicon may alleviate salt stress in wheat due to decreased Na/K and Na/Ca ratios and proline concentration in leaves. Tirchoderma inoculation significantly increased chlorophyll content and grain yield of wheat under salt stress. Trichoderma virens deteriorate salt stress by significantly decreasing Na/K and Na/Ca ratios and proline concentration and increasing soluble sugar in the leaves.


Ahmad R, Zaheer S and Ismail S, 1992. Role of silicon in salt stress tolerance of wheat (Triticum aestivum L.). Plant Science 85: 43-50.
Al-Garni SMS, 2006. Increasing NaCl-salt tolerance of a halophytic plant Phragmites australis by mycorrhizal symbiosis. American-Eurasian Journal of Agricultural and Environmental Science 1: 119–126.
Ashraf M and Foolad MR, 2007. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany 59: 206-216.
Assaha DVM Ueda A,  Saneoka H, Al-Yahyai R and Yaish MW, 2017. The role of Na+ and K+ transporters in salt stress adaptation in glycophytes. Frontiers in Physiology 8: 509. doi:10.3389/fphys.2017.00509.
Bae H, Sicher R, Kim M, Kim SH, Strem MD, Melnick R andBailey B, 2009. The beneficial endophyte Trichoderma hamatum isolate DIS 219b promotes growth and delays the onset of the drought response in Theobroma cacao. Experimental Botany 60: 3279-3295.
Bates LS, Waldren  RP and Teare  ID, 1973. Rapid determination of free proline for water-stress studies. Plant and Soil 3: 205-207.
Broadley M, Brown P, Cakmak I, Ma JF, Rengel Z and Zhao F, 2012. Beneficial elements. In: Marschner P (ed.). Marschner's Mineral Nutrition of Higher Plants. Pp. 249-269. Elsevier, Oxford, UK.
Chen TH and Murata N, 2002. Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes. Current Opinion in Plant Biology 5: 250-257.
Datta JK, NagS, Banerjee A and Mondai NK, 2009. Impact of salt stress on five varieties of wheat (Triticum aestivum L.) cultivars under laboratory condition. Journal of AppliedSciences and Environmental Management 13:93-97.
de Lacerda C, Cambraia  J, Oliva MA, Ruiz AH and Tarquı́nio Prisco J, 2003. Solute accumulation and distribution during shoot and leaf development in two sorghum genotypes under salt stress. Environmental and Experimental Botany 49:107-120.
Dubois M, Gilles K,  Ko Hamilton J, Rebers P and Smith F, 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28: 350-356.
Etesami Hand Beattie GA, 2018. Mining halophytes for plant growth-promoting halotolerant bacteria to enhance the salinity tolerance of non-halophytic crops. Frontiers in Microbiology 9: 148. doi: 10.3389/fmicb.2018.00148.
Fauteux F, Chain F, Belzile F, Menzies JG and Bélanger RR, 2006. The protective role of silicon in the Arabidopsis-powdery mildew pathosystem. Proceedings of the National Academy of Sciences 103: 17554-17559.
Gharsallah C, Fakhfakh H, Grubb D and Gorsane F, 2016. Effect of salt stress on ion concentration, proline content, antioxidant enzyme activities and gene expression in tomato cultivars. AoB PLANTS 8, plw055,
 Gong HJ, Randall DP and Flowers TJ, 2006. Silicon deposition in the root reduces sodium uptake in rice (Oryza sativa L.) seedlings by reducing bypass flow. Plant, Cell and Environment 29: 1970-1976.
Gupta PK, 2004. Soil, Plant, Water and Fertilizer Analysis. Agrobios, India, pp. 438.
Harman G, Charles E, Howell R, ViterboA, Chet I and  Lorito  M, 2004. Trichoderma species-opportunistic, avirulent plant symbionts. Nature Reviews Microbiology 2:43-45.
Hu T, Chen K, Hu L, Amombo E and Fu J,2016. H2O2 and Ca2+-based signaling and associated ion accumulation, antioxidant systems and secondary metabolism orchestrate the response to NaCl stress in perennial ryegrass. Scietific Reports 6: 36396. doi:10.1038/srep36396.
Jamil A,  Riaz S, Ashraf M and Foolad MR , 2011. Gene expression profiling of plants under salt stress. Critical Reviews in Plant Sciences 30:435-458.
Kader M and Lindberg S, 2010. Cytosolic calcium and pH signaling in plants under salinity stress. Plant Signaling and Behaviour5: 233-238.
Khan MA, Shirazi MU, Mujtaba SM, Islam E, Mumtaz S, Shereen A, Ansari RU and Yasin Ashraf M, 2009. Role of proline, K/NA ratio and chlorophyll content in salt tolerance of wheat (Triticum aestivum L.). Pakistan Journal of Botany 41(2): 633-638.
Kim YH, Khan AL, Kim DH, Lee SY, Kim KM and Waqas M, 2014. Silicon mitigates heavy metal stress by regulating P-type heavy metal ATPases, Oryza sativa low silicon genes and endogenous phytohormones. BMCPlant Biology14: 13. doi:10.1186/1471-2229-14-13.
Lee SK, Sohn EY, Hamayun M, Yoon JY and Lee IJ, 2010. Effect of silicon on growth and salinity stress of soybean plant grown under hydroponic system. Agroforestry Systems 80: 333-340.
Liang Y, 1999. Effects of silicon on enzyme activity and sodium, potassium and calcium concentration in barley under salt stress. Plant and Soil 209:217-224.
Liang Y, Zhang W, Chen Q,  Liu Y and Ding R, 2006.Effect of exogenous silicon (Si) on H+-ATPase activity, phospholipids and fluidity of plasma membrane in leaves of salt-stressed barley(Hordeum vulgare L.). Environmental and Experimental Botany  57:212-219.
Lutts S, Majerus V and Kinet JM, 1999. NaCl effects on proline metabolism in rice (Oryza sativa) seedlings. Physiolgia Plantarum 105:450-458.
Luyckx  M, Hausman JF, Lutts S and Guerriero G, 2017. Silicon and plants: current knowledge and technological perspectives. Frontiers in Plant Science 23: 411-418.
Ma JF and Takahashi E, 2002. Functions of silicon in plant growth. In: Ma JF and Takahashi E (eds.). Soil, Fertilizer and Plant Silicon Research in Japan. 1st edition. Elsevier Science, Amsterdam, the Netherlands.
Madan S, Nainawatte HS, Jain PK and Chowdhury JB, 1995. Proline and proline metabolizing enzymes in vitro selected NaCl-tolerant Brassica juncea L. under salt stress. Annals of Botany 76: 51-57.
Moussa H, 2006. Influence of exogenous application of silicon on physiological response of salt-stressed maize (Zea mays L.). International Journal of Agriculture and Biology 8: 293-297.
Nemati  I, Moradi F, Gholizadeh S, Esmaeili MA and BihamtaMR, 2011. The effect of salinity stress on ions and soluble sugars distribution in leaves, leaf sheaths and roots of rice (Oryza sativa L.) seedlings. Plant, Soil andEnvironment57: 26-33.
Netondo GW, Onyango JC and Beck E, 2004. Sorghum and salinity: I. Response of growth, water relations, and ion accumulation to NaCl salinity. Crop Science 44(3): 797-805.
Parvaiz A and Satyawati S, 2008. Salt stress and phyto-biochemical responses of plants- a review. Plant, Soil andEnvironment 54:89-99.
Pei ZF, Ming DF,  Liu D, Wan GL,  Geng XX, Gong  HJ and Zhou  WJ, 2010. Silicon improves the tolerance to water-deficit stress induced by polyethylene glycol in wheat (Triticum aestivum L ( seedlings. Journal of Plant Growth Regulation 29: 106-115.
Porcel R and Ruiz-Lozano JM, 2004. Arbuscular mycorrhizal influence on leaf water potential, solute accumulation and oxidative stress in soybean plants subjected to drought stress. Journal of Experimental Botany 55: 1743-1750.
Rawat L, Yingh Y, Shukla N and  Kumar  J, 2011. Alleviation of the adverse effects of salinity stress in wheat (Triticum aestivum L.) by seed biopriming with salinity tolerant isolates of Trichoderma harzianum. Plant and Soil 347: 387-400.
Romero-ArandaM, Jurado O and Cuartero  J, 2006. Silicon alleviates the deleterious salt effect on tomato plant growth by improving plant water status. Journal of Plant Physiology163:847-855.
Saqib M, Zörb C and Schubert S, 2008. Silicon-mediated improvement in the salt resistance of wheat (Triticum aestivum) results from increased sodium exclusion and resistance to oxidative stress. Functional Plant Biology 35: 633-639.
SAS Institute, Inc., 2000. SAS/STAT Users Guide, Version 6.12. SAS Institute, Inc., Cary, NC, USA.
Sheng  M, Tang M, Zhang F and Huang Y,2 011. Influence of arbuscular mycorrhiza on organic solutes in maize leaves under salt stress. Mycorrhiza 21:423-430.
Shukla N, Awasthi RP, Rawat L and  KumarJ, 2012. Biochemical and physiological responses of rice (Oryza sativa L.) as influenced by Trichoderma harzianum under drought stress. Plant Physiology and Biochemistry 54:78-88.
Singh AK and Dubey RS, 1995. Changes in chlorophyll a and b contents and activities of photosystem I and II in rice seedlings induced by NaCl. Photosyntheica 31:489-499.
Sonobe K, Hattori T, An P, Tsuji, W, Egrinya Eneji A, Kobayashi S, Kawamura Y, Tanaka K, and Inanaga S, 2010.  Effect of silicon application on sorghum root responses to water stress. Journal of Plant Nutrition34:71-82.
Szabados L and Savouré A, 2010. Proline: a multifunctional amino acid. Trends in Plant Sciences 15: 89-97.
Tahir  MA, Rahmatullah T, Aziz M, Ashraf S, Kanwal S and Maqsood MA, 2006. Beneficial effects of silicon in wheat (Triticum aestivum L.) under salinity stress. Pakistan Journal of Botany 38:1715-1722.
Tale AhmadS and  Haddad  R, 2011.Study of silicon effects on antioxidant enzyme activities and osmotic adjustment of wheat under drought stress. Czech Journal of Genetics and Plant Breeding 47:17-27.
Ezz T and Nawar A, 1994. Salinity and mycorrhizal association in relation to carbohydrate status, leaf chlorophyll and activity of peroxidase and polyphenol oxidase enzymes in sour orange seedlings. Alexandria Journal of Agricultural Research 39: 263-280.
Thomson BD, Robson AD and Abbott LK, 1990. Mycorrhizas formed by Gigaspora calospora and Glomus fasciculatum on subterranean clover in relation to soluble carbohydrates in roots. New Phytologist 114: 217-225.
Tuna AL, Kaya C, Ashraf M, Altunlu H, Yokas I and Yagmur B, 2007. The effects of calcium sulfate on growth, membrane stability and nutrient uptake of tomato plants grown under salt stress. Environmental and Experimental Botany 59: 173-178.
Tuna A, KayaC, Higgs D, Murillo-Amador B,  Aydemir S and Girgin, 2008. Silicon improves salinity tolerance in wheat plants. Environmental and Experimental Botany 62: 10-16.
Turan MA, Elkarim AHA, Taban N and Taban S, 2010. Effect of salt stress on growth and ion distribution and accumulation in shoot and root of maize plant. African Journal of Agricultural Research 5: 584-588.
Wang XS and HanJG, 2007.Effects of NaCl and silicon on ion distribution in the roots, shoots and leaves of two alfalfa cultivars with different salt tolerance. Soil Science and Plant Nutrition53:278-285.
YildirimE, Taylor AG and Spittler TD, 2006. Ameliorative effects of biological treatments on growth of squash plants under salt stress. Scientia Horticulturae111: 1-6.
Yin L, Wang S, Li  J, Tanaka K and Oka M, 2013. Application of silicon improves salt tolerance through ameliorating osmotic and ionic stresses in the seedling of Sorghum bicolor. Acta Physiologiae Plantarum 35: 3099-3107.
Zarea MJ, Hajinia S, Karimi N, Mohammadi Goltapeh  E, Rejali  F and Varma A, 2012. Effect of Piriformospora indica and Azospirillum strains from saline or non-saline soil on mitigation of the effects of NaCl.  Soil Biology and Biochemistry 45:139-146.
Zhu X, Song F, Liu S and Liu F, 2016. Role of arbuscular mycorrhiza in alleviating salinity stress in wheat (Triticum aestivum L.) grown under ambient and elevated CO2. Journal of Agronomy and Crop Science 202: 486-496.
Zhu Z, Wei G, Li J,  Qian Q and Yu J, 2004.Silicon alleviates salt stress and increases antioxidant enzymes activity in leaves of salt-stressed cucumber (Cucumis sativus L.). Plant Science167:527-533.