Morphological, physiological and biochemical response of Chrysanthemum to thiamine and salicylic acid

Document Type : Research Paper


Department of Horticulture, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.


Chrysanthemum (Chrysanthemum morifolium) is a major ornamental plant with high economic importance. The effect of various rates of salicylic acid (SA) (50, 100, 150 and 200 mg l-1) and thiamine (100 and 150 mg l-1) was studied on some morphological, physiological and biochemical traits of cut chrysanthemums in an experiment on the basis of a randomized complete block design with three replications. The results showed that the highest stem diameter, stem length, flower diameter, flower number, cut flower number, carotenoid, shoot fresh weight and root uptake were related to thiamine rate of 100 mg l-1. Thiamine rate of 150 mg l-1 was associated with the highest vase life, chlorophyll a and b and total chlorophyll. The highest reduced sugar and the lowest flowering time were observed in flowers treated with 150 mg l-1 SA. Also, the highest peroxidase was related to SA rate of 200 mg l-1. In total, it was found that thiamine and SA play an important role in improving morphological, physiological and biochemical traits of cut chrysanthemums. However, different rates of these two compounds entailed various impacts.


Article Title [فارسی]

واکنش فیزیولوژیکی، مورفولوژیکی و بیوشیمیایی گل بریده داودی به تیامین و اسید سالیسیلیک

Authors [فارسی]

  • مهرداد بابا ربیع
  • حسین زارعی
  • سیما بادلی
Abstract [فارسی]

گل داوودی با نام علمی Chrysanthemum morifolium یکی از مهم‌ترین گیاهان زینتی است که دارای اهمیت اقتصادی بالایی می‌باشد. به منظور بررسی تاثیر اسید سالیسیلیک (150،100،50 و200 میلی‌گرم در لیتر) و تیامین (100 و150میلی گرم در لیتر) بر برخی صفات مورفولوژیکی، فیزیولوژیکی و بیوشیمیایی گل شاخه بریده داودی اسپری، آزمایشی در قالب طرح کاملا تصادفی با سه تکرار انجام شد. بر اساس نتایج حاصل بیشترین قطر ساقه، طول ساقه، قطر گل، تعداد گل، تعداد شاخه گل، کارتنوئید، وزن تر اندام هوایی و جذب ریشه در غلظت100 میلی‌گرم در لیتر تیامین به دست‌آمد. غلظت150میلی‌گرم در لیتر تیامین، بیشترین عمر گلجایی و کلروفیل a،b  و کل را ایجاد کرد. بیشترین قند احیا و کمترین زمان ظهور گل در گل‌های تیمارشده با اسید سالیسیلیک (150 میلی‌گرم در لیتر) مشاهده شد. همچنین بیشترین میزان آنزیم پراکسیداز مربوط به اسید سالیسیلیک (200 میلی‌گرم در لیتر) بود. به طور کلی نتایج پژوهش حاضر نشان داد که تیامین و اسید سالیسیلیک نقش بسزایی در بهبود صفات مورفولوژیکی، فیزیولوژیکی و بیوشیمیایی گل شاخه بریده داودی داشتند. در عین حال غلظت‌های مختلف هر دو ماده تاثیرات مختلفی داشتند.

Keywords [فارسی]

  • اسید سالیسیلیک
  • تیامین
  • صفات بیوشیمیایی
  • صفات فیزیولوژیکی
  • صفات مورفولوژیکی
  • گل داودی
Abd El-Aziz NG, Fatma El-Quesni EM and Farahat MM, 2007. Response of vegetative growth and some chemical constituents of Syngonium podophyllum L. to foliar application of thiamine, ascorbic acid and kinetin at Nubaria. World Journal of Agricultural Sciences 3(3): 301-305.
Alaey M, Babalar M, Naderi R and Kafi M, 2011. Effect of pre- and postharvest salicylic acid treatment on physio-chemical attributes in relation to vase-life of rose cut flowers. Postharvest Biology and Technology 61(1): 91-94.
Anwar M, Sahito HA, Hassan I, Abbasi NA, Ahmed HA, Bhatti MA, Hussain A, Iqbal Z and Abro AH, 2014. Effect of pre harvest treatment of salicylic on growth and vase life of tuberose with aroma environment. Wudpecker Journal of Agricultural Research 3(2): 50-57.
Armitage, AM and Laushman JM, 2003. Spatiality Cut Flowers: The Production of Annuals, Perennials, Bulbs, and Woody Plants for Fresh and Dried Cut Flowers. 2nd edition. Timber Press, Inc., Cambridge, UK, pp. 392.
Baniasadi F and Saffari VR, 2015. Effects of GA3, BA, thiamine and ascorbic acid on some morphological and biochemical characteristics of periwinkle (Catharanthus roseus L.). Journal of Horticultural Science 29(4): 556-563 (In Persian with English abstract).
Barnes JD, Balaguer L, Manrique E, Elvira S and Davison AW, 1992. A reappraisal of the
use of DMSO for the extraction and determination of chlorophyll a and b in lichens and higher plants. Environmental and Experimental Botany 32(2): 85-100.
Barth C, Tullio MD and Conklin PL, 2006. The role of ascorbic acid in the control of flowering time and the onset of senescence. Journal of Experimental Botany 57: 1657-1665.
Bedour AAL and Rawia AE, 2011. Improving gladiolus growth, flower keeping quality
by using some vitamins application. Journal of American Science 7(3): 169-174.
Bestwick CS, Brown IR and Mansfield JW, 1998. Localized changes in peroxidase activity accompany hydrogen peroxide generation during the development of a nonhost hypersensitive reaction in lettuce.Plant Physiology 118(3): 1067-1078.
Bidwell RGS, 1979.  Plant Physiology. 2nd edition. Macmillan Publishing Co., Inc. New York.
Corina Vlot A, Dempsey DMA and Klessig DF, 2009. Salicylic acid, a multifaceted hormone to combat disease. Annual Review of Phytopathology 47: 177-206.
Da Silva JAT, 2003. The cut flower: postharvest considerations. Journal of Biological Sciences 3(4): 406-442.
Da Silva JAT, 2004. Ornamental Chrysanthemums: improvement by biotechnology. Plant Cell, Tissue and Organ Culture79(1): 1-18.
De Capdeville G, Maffia LA, Finger FL and Batista U, 2003. Gray mold severity and vase life of rose buds after pulsing with citric acid, salicylic acid, calcium sulfate, sucrose and silver thiosulfate. Fitopatologia Brasileira 28(4): 380-385.
Dyer D, Cotterman JC, Cotterman CD, Kerr PS and Carlson DR, 1990. Cytokinins as metabolic stimulants which induce pod set. In: Pharis RP and Rood SB (eds). Plant Growth Substances 1988. Pp. 457-467. Springer, Berlin, Heidelberg, Germany.
Ezhilmathi K, Singh VP, Arora A and Sairam RK, 2007. Effect of 5-sulfosalicylic acid on antioxidant activity in relation to vase life of Gladiolus cut flowers. Plant Growth Regulation 51: 99-108.
Fathi Gh and Esmaeelpoor B, 2010. Plant Growth Regulators (Principles and Applications). Mashhad University JihadPublications, pp. 157 (In Persian).
Gapinska M, Sklodowska M and Gabara B, 2008. Effect of short- and long-term salinity on the activities of antioxidative enzymes and lipid peroxidation in tomato roots. Acta Physiologiae Plantarum 30: 11-18.
Golan-Goldhirsh A, Mozafar A and Oertli JJ, 1995. Effect of ascorbic acid on soybean seedlings grown on medium containing a high concentration of copper. Journal of Plant Nutrition 18: 1735-1741.
Goodwin TW and Britton G, 1998. Distribution and analysis of carotenoids. In: Goodwin TW (ed.). Plant Pigments. Pp. 62-132. Academic Press. London.
Grotewold E, 2006. The genetics and biochemistry of floral pigments. Annual Review of Plant Biology 57: 761-780.
Hajireza R, Hadavi A, Zeinanloo A and Mirzapoor MH, 2013. The effect of different levels of citric acid and salicylic acid in pre-harvesting time on vase life of cut roses. Journal of Science and Technology of Greenhouse Culture 4(16): 99-109 (In Persian with English abstract).
Hassanpour Asil M, Karimi HA and Mousanejad S, 2014. The effects of essential oils, nanoparticles of silver and several chemical agents on vase life of gladiolus cut flowers. Iranian Journal of Horticultural Sciences 45(4): 449-460 (In Persian with English abstract).
Hayat Q, Hayat S, Irfan M and Ahmad A, 2010. Effect of exogenous salicylic acid under changing environment: a review. Environmental and Experimental Botany 68(1): 14-25.
Hayat S, Ali B and Ahmad A, 2007. Salicylic acid: biosynthesis, metabolism and physiological role in plants. In: Hayat S and Ahmad A (eds.). Salicylic Acid: A Plant Hormone. Pp. 1-14. Springer, Dordrecht, ‎The Netherlands.
Hegazi MA, 2016. Evaluation of pre- or post-harvest application of some minerals and organic agents on the growth, flowering and vase life of Rudbeckia hirta, L. Journal of Agricultural Science 8(9): 226-236.
Hosseini H, Farahmand H and Saffari VR, 2015. The effect of foliar application of ascorbic acid, thiamine and benzyl adenineon growth, flowering and some biochemical characteristics of marigold (Tagetes erecta L.).  Journal of Plant Productions 38(2): 25-36 (In Persian with English abstract).
Jabbarzadeh Z, Khosh-Khui M and Salehi H, 2009. The effect of foliar-applied salicylic acid on flowering of African violet. Australian Journal of Basic and Applied Sciences 3(4): 4693-4696.
Kamali M, Kharrazi SM, Salahvarzi Y and Tehranifar A, 2012. Effect of salicylic acid on the growth and some of morphophysiological characteristics of Gomphrena (Gomphrena globosa L.) under salinity stress. Journal of Horticultural Science 26(1): 104-112 (In Persian with English abstract).
Kawasaki T, 1992. Modern Chromatographic Analysis of Vitamins. 2nd edition, Volume 60. Marcel Dekker, Inc., New York, NY:
Khan W, Prithiviraj B and Smith DL, 2003. Photosynthetic responses of corn and soybean to foliar application of salicylates. Journal of Plant Physiology160: 485-492.
Kheiry A, Khalighi A, Mostofi Y and Naderi R, 2011. Effects of gibberellic acid (GA3) and benzyladenine on tuberose quality and quantity. Journal of Crops Improvement 13(1): 9-20 (In Persian with English abstract).
Leslie CA and Romani RJ, 1988. Inhibition of ethylene biosynthesis by salicylic acid. Plant
Physiology 88: 833-837.
Lopez-Juez E and Pyke KA, 2005. Plastids unleashed: their development and their integration in plant development. The International Journal of Developmental Biology 49: 557-577.
Mahgoub MH, El-Aziz NGA and Mazhar AMA, 2011. Response of Dahlia pinnata L. plant to foliar spray with putrescine and thiamine on growth, flowering and photosynthetic pigments. American Eurasian Journal of Agricultural and Environmental Sciences 10(5): 769-775.
Mansouri M, Shoor M, Tehranifar A and Selahvarzi Y, 2015. Effect of foliar application of salicylic acid and thiamine on quantitative and qualitative characteristics of gerbera (Gerbera jamesonii L. cv. Pink Elegance). Journal of Science and Technology of Greenhouse Culture6(23): 15-23 (In Persian with English abstract).
Marissen N, 2001. Effects of pre-harvest light intensity and temperature on carbohydrate levels and vase life of cut roses. Acta Horticulturae 543: 331-336.
Martin-Mex R, Nexticapan-Garcez A, Villanueva-Couoh E, Uicab-Quijano V, Vergara Yoisura S and Larque-Saavedra E, 2015. Salicylic acid stimulates flowering in micropopagated gloxinia plants. Revista Fitotecnia Mexicana 38(2): 115–118.
Martin-Mex R, Villanueva-Couoh E, Herrera-Campos T and Larque-Saavedra A, 2005. Positive effect of salicylates on the flowering of African violet. Scientia Horticultrae 103(4): 499-502.
Mortazavi SN, Karimi V and Azimi MH, 2015. Pre-harvest foliar application of humic acid, salicylic acid and calcium chloride to increase quantitative and qualitative traits of Lilium longiflorum cut flowers.  Journal of Science and Technology of Greenhouse Culture6(23): 37-46 (In Persian with English abstract).
Mortazavi N, Naderi R, Khalighi A, Babalar M and Allizadeh, H, 2007. The effect of cytokinin and calcium on cut flower quality in Rose (Rosa hybrida cv. Illona). Journal of Food, Agriculture and Environment 5(3-4): 311-313.
Nabigol A, Ghodsi Sh and Hadavi E, 2016. The effect of putrescine and salicylic acid on growth and flowering characteristics of Dahlia. 1st International and 2nd National Ornamental Plants Congress, 23-25 August, Ferdowsi University, Mashhad, Iran, pp. 1-5.
Oertli JJ, 1987. Exogenous application of vitamins as regulators for growth and development of plants: a review. Journal of Plant Nutrition and Soil Science 150: 375-391.
Pasandipour A, Farahbakhsh H, Saffari M and Karamat B, 2013. The effect of salicylic acid on some physiological reactions of fenugreek (Trigonella foenum-graecum) under salinity stress. Journal of Crop Ecophysiology 26(2): 215-228 (In Persian with English abstract).
Price CE, 1966. Ascorbate stimulation of RNA synthesis. Nature 212: 1481. doi:10.1038/2121481a0.
Roein Z, Hassanpour Asil M and Sabouri A, 2015. Morphological characteristics, genetic diversity and classification of Chrysanthemum genotypes. Journal of Crop Production and processing 5(16): 345-360 (In Persian with English abstract).
Romani RJ, Hess BM and Leslie CA, 1989. Salicylic acid inhibition of ethylene production by apple discs and
other plant tissues. Journal of Plant Growth Regulation 8: 63-69.
Roustan JP, Latche A and Fallot, 1989. Stimulation of Daucus carota somatic embryogenesis by inhibitors of ethylene synthesis: cobalt and nickel. Plant Cell Reports 8(3): 182-185.
Salehi M, Safari VR and Farahmand H, 2016. The effect of foliar application of benzyl adenine, ascorbic acid and thiamine on some morphological and biochemical characteristics of petunia (Petunia hybrida). Journal of Crop Production and Processing, 6(19): 165-175 (In Persian with English abstract).
Samadi S, Ghasemnezhad A, Alizadeh M and Alami M, 2016. Influence of elicitors on photosynthesis pigments, caffeic acid, chlorogenic acid and proline of Cynara scolymus callus. Iranian Journal of Horticultural Science 47(3): 435-443 (In Persian with English abstract).
Seyed Hajizadeh H and Aliloo AA, 2013. The effectiveness of pre-harvest salicylic acid application on physiological traits in lilium (Lilium longiflorum L.) cut flower. International Journal of Scientific Research in Environmental Sciences 1(12): 344-350.
Somogyi M, 1952. Notes on sugar determination. Journal of Biological Chemistry 195: 19-29.
Vanacker H, Lu H, Rate DN and Greenberg JT, 2001. A role for salicylic acid and NPR1 in regulating cell growth in Arabidopsis. The Plant Journal 28(2): 209-216.
Van Doom WG and Perik RRJ, 1990. Hydroxyquinoline citrare and low pH prevent vascular blockage in stems of cut rose flowers by reducing the number of bacteria. American Society for Horticultural Sciences 115(6): 979-981.
Weinstein LH, 1951. Senescence of roses I. Chemical changes associated with senescence of cut ‘Better times’ roses. Contributions from Boyce Thompson Institute 19: 33-48.
Zhang Y, Chen K, Zhang S and Ferguson I, 2003. The role of salicylic acid in postharvest ripening of kiwifruit. Postharvest Biology and Technology 28(1): 67-74.