Effect of plant growth regulators on Fittonia verschaffeltii regeneration at in vitro conditions

Document Type : Research Paper

Authors

1 Department of Agricultural Biotechnology, Faculty of Agriculture, Islamic Azad University, Damghan Branch, Damghan, Iran.

2 PhD student of Plant Breeding, Department of Agronomy and Plant Breeding, Young Researchers and Elite Club, Karaj Branch, Islamic Azad University, Karaj, Iran.

3 Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, Islamic Azad University, Karaj Branch, Karaj, Iran.

4 Genetic and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resource University, Sari, Iran.

Abstract

We investigated in vitro regeneration ability of Fittonia verschaffeltii using different explants (leaf, node) and plant growth regulators ((2,4-D, indoleacetic acid, indolebutyric acid (IBA), 6-benzylaminopurine (BAP), thidiazuron)). Percentage of callus induction, number of shoots, shoot length, percentage of rooting and root length were measured. The leaf explant showed higher frequency of callus induction in comparison with the node explant, while node had higher frequency of shoot formation. There were significant differences among plant growth regulators in terms of percentage of callus induction, number of shoots, percentage of rooting and root length, however, the difference was dependent on the type of explant. The highest percentage of callus induction (86.12%) was obtained for the leaf explant at Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-D and 1 mg/L IBA. The highest number of shoots (5.9 per explant) and shoot length (7.95 cm) were observed for the node explant at MS medium supplemented with 2 mg/L BAP + 1 mg/L IBA and MS medium containing 1mg/L BAP + 1 mg/L IBA, respectively. The highest percentage of root formation (87%) and root length (10.1 cm) were obtained at MS medium supplemented with 0.2 mg/L IBA and MS medium with no hormones, respectively.
 
 

Keywords


Article Title [فارسی]

تأثیر تنظیم‌کننده‌های رشد بر باززایی گیاه فیتونیا (Fittonia verschaffeltii) در شرایط درون شیشه‌ای

Authors [فارسی]

  • مهسا رشیدی 1
  • علی صارمی راد 2
  • ولی اله قاسم عمران 4
Abstract [فارسی]

در پژوهش حاضر به بررسی توانایی باززایی گیاه فیتونیا با به کارگیری ریزنمونه‌های مختلف (برگ، گره) در غلظت‌های متفاوت تنظیم‌کننده‌های رشدی گوناگون ((2,4-D، ایندول استیک اسید، ایندول بوتیریک اسید (IBA)، 6-بنزیل آمینو پورین (BAP) و تیدیازورون)) پرداخته شد. درصد القای کالوس، تعداد شاخساره، طول شاخساره، درصد ریشه‌زایی و طول ریشه یادداشت‌برداری شدند. تفاوت قابل ملاحظه‌ای میان تنظیم‌کننده‌های رشدی از لحاظ درصد القای کالوس، تعداد شاخساره، درصد ریشه‌زایی و طول ریشه وجود داشت. نتایج حاصل نشان داد که ریزنمونه برگ از بیشترین درصد القای کالوس در مقایسه با گره برخوردار است در حالی که گره بیشترین تعداد شاخساره را داشت. بیشترین درصد القا (12/86 درصد) مربوط به ریزنمونه برگ در محیط کشت MS حاوی یک میلی‌گرم در لیتر 2,4-D و یک میلی‌گرم در لیتر IBA بود. ریزنمونه گره در محیط کشت MS حاوی دو میلی‌گرم در لیتر BAP و یک میلی‌گرم در لیتر IBA دارای بیشترین تعداد شاخساره (9/5 در هر ریزنمونه) و در محیط کشت MS حاوی یک میلی‌گرم در لیتر BAP و یک میلی‌گرم در لیتر IBA دارای بیشترین طول شاخساره (95/7 سانتی‌متر) بود. بالاترین درصد ریشه‌زایی (87 درصد) و طول ریشه (1/10 سانتی‌متر) به ترتیب در MS غنی شده با 2/0 میلی‌گرم در لیتر IBA و محیط کشت MS پایه به دست آمد.
 

Keywords [فارسی]

  • القای کالوس
  • تنظیم‌کننده رشد
  • ریزنمونه
  • کشت درون-شیشه­ای
  • Fittonia verschaffeltii
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