Effects of various nitrogen sources on synchronization of tomato somatic embryogenesis during induction and realization phases

Document Type : Research Paper


1 Department of Horticultural Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

2 Department of Agronomy, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.


Tissue culture through somatic embryogenesis is one of the methods found most useful in the plants’ breeding process. A key issue to deal with during somatic evolution is its synchronization. Nitrogen has been known to play an important role here. Therefore, we evaluated the tomato explant embryogenesis cultured on B5 basal medium, subject to oxidized, reduced and organic nitrogen. Two separate experiments were conducted consisting of eight treatments with four replications each, using completely randomized design. In the experiment in which treatments were applied during the induction phase, maximum synchrony, based on relative number of torpedo embryo to all formed embryos, was obtained using nitrate as the sole nitrogen source (0.81). However, in the case in which treatments were applied during the realization phase, maximum synchrony was obtained through the combined nitrate and casein hydrolysate (0.51). Furthermore, in both experiments the highest number of somatic embryos was obtained in the standard B5 medium (91.58 in the first experiment and 59.19 in the second experiment).


Article Title [Persian]

اثر منابع مختلف نیتروژن روی هم‌زمان سازی جنین زایی رویشی گوجه فرنگی در طی مراحل القا و ظهور جنین

Authors [Persian]

  • آیدا شمالی 1
  • کامبیز مشایخی 1
  • محمد هادی پهلوانی 2
  • سید جواد موسوی زاده 1
Abstract [Persian]

کشت بافت از طریق جنین زایشی رویشی یکی از روش­هایی است که بیشتر در روند اصلاح گیاهان کاربرد دارد. یک مسئله کلیدی جنین زایی رویشی، هم­زمان سازی آن است. مشخص شده است که نیتروژن در این مورد نقش مهمی ایفا می­کند. بنابراین، آزمایشی برای ارزیابی جنین زایی رویشی گوجه فرنگی در محیط پایه B5، تحت تاثیر نیتروژن اکسیدی، احیایی و آلی قرار گرفت. دو آزمایش جداگانه شامل هشت تیمار با چهار تکرار هر کدام با استفاده از طرح کاملا تصادفی انجام شد. در آزمایشی که تیمارها در طول مرحله القا، اعمال شده بودند، حداکثر هم­زمان سازی براساس تعداد نسبی جنین اژدری شکل به تمام جنین­های تشکیل دهنده جنین، با استفاده از نیترات به عنوان تنها منبع نیتروژن (81/0) به دست آمد. در مقابل، زمانی که در طول مرحله ظهور جنین تیمارها اعمال شدند، حداکثر هم­زمان سازی با استفاده از ترکیب نیترات و هیدرولیزات کازئین (51/0) به دست آمد. علاوه بر این، در هر دو آزمایش بیشترین تعداد جنین رویشی در محیط استاندارد B5 (58/91 در اولین آزمایش و 19/59 در آزمایش دوم) ثبت شد.

Keywords [Persian]

  • آمونیوم
  • درون شیشه ای
  • کازئین هیدرولیزات
  • گوجه فرنگی
  • نیترات
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