In vitro regeneration in medicinal plant fenugreek (Trigonella foenum-graecum L.)

Document Type : Research Paper


Department of Agriculture, Payame Noor University


Fenugreek (Trigonella foenum-graecum L.) is a medicinal plant used as a traditional medicine. Two of the most important medicinal components are diosgenin and trigonelline that have many medicinal properties including anticancer, decreasing blood sugar and cholesterol. In vitro optimization of callus induction and shoot regeneration is the first step in the optimum production of secondary metabolites through plant tissue culture techniques and genetic engineering. To achieve this goal, leaf, hypocotyl and embryo axis explants were cultured on Murashige-Skoog (MS) medium containing various concentrations of naphthalene acetic acid (NAA) (0, 0. 5, 1.5 and 2 mg L-1) in combination with 6-benzyl adenine (BA) (0, 0.5, 1 and 1.5 mg L-1) producing 48 treatments. The data were analyzed by the non-parametric Kruskal-Wallis test and the means were compared by the U test of Mann Whitney at 5% probability level. The result of the analysis indicated a significant difference between treatments. Simultaneous callus induction and shoot regeneration occurred in the MS medium supplemented with 0.5 mg L-1 NAA withoutBA for the embryo axis explant. However, higher frequency of callus induction for leaf and hypocotyl explants was obtained in the higher concentration of NAA (2 mg L-1).


Article Title [Persian]

باززایی درون شیشه در گیاه دارویی شنبلیله (Trigonella foenum-graecum L.)

Author [Persian]

  • محمود ولیزاده
Abstract [Persian]

شنبلیله (Trigonella foenum-graecum L.) یک گیاه دارویی بوده که در طب سنتی مورد استفاده قرار می­گیرد. دو تا از مهمترین مواد دارویی دیوزجنین و تری گونلین می­باشد که دارای خواص دارویی بسیار از جمله خاصیت ضد سرطان، پایین آورنده کلسترول و قند خون می­باشد. بهینه سازی درون شیشه، القای کالوس و باززایی اندام­های هوایی اولین گام در جهت تولید بهینه متابولیت­های ثانویه در این گیاه از طریق کشت بافت و مهندسی ژنتیک می­باشد. به منظور دستیابی به این هدف ریزنمونه‌های برگ، هیپوکوتیل و محور جنینی روی محیط کشت موراشیک و اسکوگ (MS) حاوی غلظت‌های مختلف هورمون‌های نفتالین استیک اسید (NAA) (0، 5/0، 5/1 و 2 میلی‌گرم در لیتر) در ترکیب با 6-بنزیل‌آدنین (BA)(0، 5/0، 1 و 5/1 میلی‌گرم در لیتر) با تشکیل 48 تیمار کشت شدند. داده­ها به وسیله آزمون غیرپارامتری کروسکال والیس تجزیه شدند و میانگین­ها توسط آزمون غیرپارامتری توکی مقیسه شدند. نتایج تجزیه داده­ها تفاوت معنی­داری را بین تیمارها نشان داد. القای کالوس و باززایی اندام­های هوایی به طور هم­زمان در محیط کشت MS حاوی 5/0 میلی‌گرم در لیتر NAA بدون BA و در ریزنمونه محور جنینی رخ داد. بیشترین فراوانی القای کالوس در ریزنمونه برگ و هیپوکوتیل در غلظت بالاتر NAA  (2 میلی گرم در لیتر) به دست آمد.

Keywords [Persian]

  • القای کالوس
  • باززایی گیاه
  • شنبلیله
  • محور جنینی
  • NAA
  • BA
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