Effect of short- and long-time salt treatment on root traits and expression pattern of Atls1 gene in barley (Hordeum vulgare L.)

Document Type : Research Paper


1 Departement of Biology, Faculty of Natural Science, Azarbaijan Shahid Madani University, Tabriz, Iran.

2 Departement of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.


Barley (Hordeum vulgare L.) as a salt-tolerant crop species has considerable economic importance in salinity-affected arid and semiarid regions of the world. In the present study, three barley genotypes (Sahara3771 and an Iranian advanced line as salt tolerant and Clipper as salt susceptible) were exposed to 100 and 200 mM NaCl at the seedling stage and non-NaCl treatment was used as check. The root samples were harvested 24 hours, three days and three weeks after salt treatment in three replications. The root length and root fresh and dry weight were measured and expression pattern of Atls1 gene was analyzed on root samples by quantitative Real-time-PCR. The effects of genotype and sampling time were significant for root characters and with the advancement of salt treatment duration, root length and root fresh and dry weight were significantly reduced. The expression of Atls1 gene was significantly affected by NaCl level, genotype, sampling time and their interactions. In all three genotypes, with the increase of NaCl concentration, expression of Atls1 gene was reduced. Under 100 mM NaCl, mRNA level of Atls1 was significantly decreased in Clipper as compared with the salt tolerant genotypes, Sahara3771 and Advanced line. In addition, long-term salt treatment (three weeks) significantly reduced the expression of Atls1 in all three genotypes. Down-regulation of Atls1 gene under long term salt treatment indicates that this gene may be involved in response to salinity stress at the beginning of salt stress.


Article Title [فارسی]

تاثیر تنش شوری کوتاه و بلند مدت بر صفات ریشه و الگوی بیان ژن Atls1 در جو (Hordeum vulgare L.)

Authors [فارسی]

  • سارا غفاریان 1
  • سید ابوالقاسم محمدی 2
  • محمود تورچی 2
Abstract [فارسی]

جو (Hordeum vulgare L.) به عنوان یک گیاه متحمل به شوری دارای اهمیت اقتصادی قابل توجهی در نواحی خشک و نیمه خشک است. در این مطالعه، سه ژنوتیپ جو (Sahara3771 و لاین امید بخش به عنوان لاین­های متحمل و Clipper به عنوان لاین حساس به شوری) در مرحله گیاهچه تحت تیمار شوری صفر، 100 و 200 میلی مولار NaCl قرار گرفتند. 24 ساعت، سه روز و سه هفته پس از اعمال تیمار شوری نمونه برداری از ریشه در سه تکرار انجام شد. طول ریشه و وزن خشک و تر ریشه اندازه­گیری شد و الگوی بیان ژن Atls1 در نمونه­های ریشه با تکنیک Real-time-PCR کمی مورد بررسی قرار گرفت. اختلاف بین ژنوتیپ­ها و مراحل نمونه برداری برای صفات ریشه معنی­دار بود. با پیشرفت مراحل نمونه برداری و طول مدت تیمار، طول ریشه و وزن خشک و تر آن افزایش معنی­داری داشت. شوری، ژنوتیپ، مرحله نمونه برداری و اثر متقابل آن­ها بیان ژن Atls1 را به طور معنی­داری تحت تاثیر قرار دادند. در هر سه ژنوتیپ، با افزایش غلظت NaCl، بیان ژن Atls1 کاهش یافت. تحت شوری 100 میلی مولار NaCl، سطح mRNA ژن Atls1 در Clipper در مقایسه با ژنوتیپ­های متحمل به شوری Sahara3771 و لاین امید بخش کاهش معنی­داری داشت. علاوه بر این، در تمامی ژنوتیپ­ها تحت تیمار شوری طولانی مدت (سه هفته) بیان ژن Atls1 کاهش یافت. کاهش بیان این ژن در تیمار شوری بلند مدت نشان می­دهد که احتمالا این ژن در مراحل ابتدایی پاسخ به تنش شوری دخالت دارد.

Keywords [فارسی]

  • تنش شوری
  • جو
  • رشد ریشه
  • ژن Atls1
  • Real-Time PCR
Al-Karaki GN, 2001. Germination, sodium and potassium concentrations of barley seeds as influenced by salinity. Journal of Plant Nutrition 24: 511-522.
Giehl RF, Gruber BD and von Wirén N, 2014. It's time to make changes: modulation of root system architecture by nutrient signals. Journal of Experimental Botany 65: 769-778.
Greenway H, 1965. Plant response to saline substrates. Growth and ion uptake throughout plant development in two varieties of Hordeum vulgare. Australian Journal of Biological Science 18: 763-779.
James RA, Blake C, Byrt CS and Munns R, 2011. Major genes for Na+ exclusion, Nax1 and Nax2 (wheat HKT1;4 and HKT1;5), decrease Na+ accumulation in bread wheat leaves under saline and water logged conditions. Journal of Experimental Botany 62: 2939-2947.
Julkowska MM and Testerink C, 2015. Tuning plant signaling and growth to survive salt. Trends in Plant Science 20: 586-594.
Khan MA, Gemenet DC and Villordon A, 2016. Root system architecture and abiotic stress tolerance: current knowledge in root and tuber crops. Frontiers in Plant Science 7: 1-13.
Ladeiro B, 2012. Saline agriculture in the 21st century: using salt contaminated resources to cope food requirements. Journal of Botany 2012: 310705, http://dx.doi.org/10.1155/2012/310705.
Mahajan S and Tuteja N, 2005. Cold, salinity and drought stresses: an overview. Archives of Biochemistry and Biophysics 444: 139-158.
Munns R, 2005. Genes and salt tolerance: bringing them together. New Phytologist 167: 645-663.
Munns R and Tester M, 2008. Mechanisms of salinity tolerance. Plant Biology 59: 651-681.
Ozturk ZN, Talame V, Deyholos M, Michalowski CB, Galbraith DW, Gozukirmizi N, Tuberosa R and Bohnert HJ, 2002. Monitoring large-scale changes in transcript abundance in drought- and salt-stressed barley. Plant Molecular Biology 48: 551-573.
Paez-Garcia A, Motes CM, Scheible W, Chen R, Blancaflor, EB and Monteros MJ, 2015. Root traits and phenotyping strategies for plant improvement. Plants 4: 334-355.
Qian G, Han Z, Zhao T, Deng G, Pan Z and Yu M, 2007. Genotypic variability in sequence and expression of HVA1 gene in Tibetan hulless barley, Hordeum vulgare ssp. vulgare, associated with resistance to water deficit. Australian Journal of Agricultural Research 58: 425-431.
Rahnama A, James RA, Poustini K and Munns R, 2010. Stomatal conductance as a screen for osmotic stress tolerance in durum wheat growing in saline soil. Functional Plant Biology 37: 255-263.
Ristova D and Busch W, 2014. Natural variation of root traits: from development to nutrient uptake. Plant Physiology 166: 518–527.
Sakurai J, Ishikawa F, Yamaguchi T, Uemura M and Maeshima M, 2005. Identification of 33 rice aquaporin genes and analysis of their expression and function. Plant Cell Physiology 46: 1568-1577.
Temel A and Gozukirmizi N, 2015. Physiological and molecular changes in barley and wheat under salinity. Applied Biochemistry and Biotechnology 175: 2950-2960.
Tester M and Davenport R, 2003. Na+ tolerance and Na+ transport in higher plants. Annals of Botany 91: 503-527.
Ueda A, Kathiresan A, Bennett J and Takabe T, 2006. Comparative transcriptome analyses of barley and rice under salt stress. Theoretical and Applied Genetics 112: 1286-1294.
Ueda A, Shi W, Nakamura T and Takabe T, 2002. Analysis of salt-inducible genes in barley roots by differential display. Journal of Plant Research 115: 119-130.
Urano K, Kurihara Y, Seki M and Shinozaki K, 2010. Omics’ analyses of regulatory networks in plant abiotic stress responses. Current Opinion in Plant Biology 13: 132-138.
Widodo PJH, Patterson JH, Newbigin E, Tester M, Bacic A and Roessner U, 2009. Metabolic responses to salt stress of barley (Hordeum vulgare L.) cultivars, Sahara and Clipper, which differ in salinity tolerance. Journal of Experimental Botany 60: 4089-4103.
Yan S, Tang Z, Su W and Sun W, 2005. Proteomic analysis of salt stress-responsive proteins in rice root. Proteomics 5: 235-244.