Embryogenesis in medicinal plant Syrian Rue (Peganum harmala L.)

Document Type : Research Paper


Department of Agriculture, Payame Noor University, Iran


Peganum harmala L. is a shrub perennial plant of Zigophyllaceae. It is used for treatment of parkinson in folk medicine and has antitumor and antioxidant activity. This species is usually propagated by seeds but the span of seed viability is short. On the other hand, since this is a wild plant and is not cultivated, it is exposed to extinction due to overuse. In order to solve this problem and optimize secondary metabolite production in this plant, the first step is in-vitro optimization of callus induction and shoot regeneration. To achieve this goal, leaf, hypocotyl and embryo axis were cultured on MS medium containing different concentrations of 2,4-D (0, 0.25 and 0.5 mg L-1) in combination with BA (0, 0.5, 1 and 2 mg L-1). The results of analysis of variance showed that the main effects of hormones and explants and some interactions were significant on callus induction and shoot regeneration. Maximum callus induction and shoot regeneration was obtained in the medium supplemented with 0.5 mg L-1 2,4-D and 1 mg L-1 BA for the leaf and hypocotyl explants, respectively.


Article Title [فارسی]

بهینه سازی کشت بافت گیاه دارویی اسپند

Author [فارسی]

  • محمود ولیزاده
گروه کشاورزی، دانشگاه پیام نور
Abstract [فارسی]

گونه اسپند با نام علمی Peganum harmala گیاهی علفی و چند ساله از تیره Zigophyllaceae می‌باشد. این گونه در طب سنتی برای درمان پارکینگسون مورد استفاده قرار گرفته و دارای اثرات ضد تومور و فعالیت آنتی‌اکسیدانی است. اسپند به طور معمول به وسیله بذر تکثیر می‌شود، ولی قدرت زنده‌مانی بذر کوتاه است. از سوی دیگر، از آن جایی‌که این گیاه به صورت وحشی می‌روید و کشت نمی‌شود، به دلیل استفاده بی‌رویه در معرض خطر انقراض قرار دارد. برای رفع این مشکل و تولید بهینه متابولیت‌های ثانویه این گیاه، اولین گام بهینه‌سازی القای کالوس و باززایی اندام‌های هوایی در محیط درون شیشه می‌باشد. به این منظور ریزنمونه‌های برگ، هیپوکوتیل و محور جنینی در محیط کشت MS حاوی غلظت‌های مختلف از هورمون‌های -D2,4 (0، 25/0 و 5/0 میلی‌گرم در لیتر) و BA (0، 5/0، 1 و 2 میلی‌گرم در لیتر) کشت داده شدند. نتایج نشان داد که بیشترین مقدار القای کالوس و باززایی به طور هم­زمان، در تیمار هورمونی 5/0 میلی‌گرم در لیتر -D2,4 و 1 میلی‌گرم در لیتر BA در ریزنمونه برگ و بعد از آن هیپوکوتیل رخ داده است.

Keywords [فارسی]

  • اسپند؛ باززایی؛ کالوس؛ محیط کشتMS ؛-D 2
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