Comparative study of two pear cultivars to PEG-induced osmotic stress

Document Type : Research Paper

Authors

1 Department of Horticultural Science, University of Zanjan, Zanjan, Iran

2 Temperate Fruits Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

3 Research Institute of Modern Biological Techniques, University of Zanjan, Zanjan, Iran

Abstract

We studied the effect of polyethylene glycol (PEG)-induced osmotic stress on pear cultivars (Harrow Sweet and Bartlett) under in-vitro culture conditions. Explants were cultured in QL medium containing 4% and 8% PEG6000 using a factorial experiment based on completely randomized design. The medium without PEG was considered as control. Drought injury index, which was calculated based on morphological disorders, increased at 8% PEG nearly to 2.00 and 1.50 units in CVs. Bartlett and Harrow Sweet, respectively. Owing to osmotic stress, the increase of malondialdehyde was accompanied with the reduction of cell membrane stability index in both cultivars. Total phenolic components and antioxidant activity in leaves increased significantly in response to application of 4% and 8% PEG. However, the severity of increase was higher in CV. Harrow Sweet. It was revealed that CV. Harrow Sweet had higher tolerance to osmotic stress than Bartlett. Moreover, the parameters related to oxidative damages and ROS scavenging capacity were more descriminant against osmotic stress under in vitro system.
 

Keywords


Article Title [Persian]

مقایسه پاسخ ریزنمونه‌های دو رقم گلابی به تنش اسمزی ناشی از پلی‌اتیلن‌گلیکول

Authors [Persian]

  • وحید بیگدلو 1
  • علی سلیمانی 1
  • حمید عبدالهی 2
  • عباس بهاری 3
1 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان
2 مرکز تحقیقات درختان میوه معتدله، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج
3 پژوهشکده فناوری‌های نوین زیستی، دانشگاه زنجان، زنجان
Abstract [Persian]

در این تحقیق تاثیر تنش اسمزی ناشی از کاربرد پلی­اتیلن­گلیکول (PEG) روی ریزنمونه­های گلابی ارقام هروسوئیت و بارتلت در شرایط کشت درون­شیشه­ای مورد مطالعه قرار گرفت. ریزنمونه­ها در محیط کشت QL حاوی 4 و 8 درصد PEG6000 در قالب آزمایش فاکتوریل بر پایه طرح کاملا تصادفی کشت شدند. محیط کشت فاقد پلی­اتیلن­گلیکول به­ عنوان شاهد در نظر گرفته شد. شاخص آسیب خشکی، که بر اساس آسیب­های فیزیولوژیکی ظاهری تعیین شد، در تیمار 8 درصد PEG حدود 2 و 5/1 واحد به­ترتیب در ارقام بارتلت و هروسوئیت افزایش نشان داد. تنش اسمزی سبب کاهش پایداری غشاء سلولی و متعاقباٌ افزایش ترکیب مالون­د-آلدهید بافت برگ در هر دو رقم شد. مقدار فنول کل و ظرفیت آنتی­اکسیدانی بافت برگ ریزنمونه­ها به طور معنی­داری در مواجهه با کاربرد 4 و 8 درصد PEG افزایش یافت. لیکن شدت این افزایش در رقم هروسوئیت بیشتر بود. بر اساس نتایج این آزمایش، ریزنمونه­های گلابی رقم هروسوئیت تحمل بیشتری به تنش اسمزی نشان دادند. ­علاوه بر این، متغیرهای مرتبط با آسیب­های اکسیداتیو و ظرفیت جاروبی رادیکال­های آزاد اکسیژنی برای مطالعات مقایسه­ای تحمل به تنش اسمزی در شرایط کشت درون­شیشه­ای کارآمدی بیشتری نشان دادند.
 

Keywords [Persian]

  • پرولین
  • تنش خشکی
  • سوپراکسید دیسموتاز
  • کشت درون شیشه­ای
  • Pyrus communis
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