Effects of drought stress on some physiological variables and grain yield of different wheat varieties

Document Type : Research Paper


1 PhD student of Agronomy and Plant Breeding, University of Birjand, Birjand, Iran and former MSc student, Shahid Bahounar University, Kerman, Iran

2 Agronomy and Plant Breeding, Shahid Bahounar University of Kerman, Kerman, Iran

3 Agronomy and Plant Breeding, University of Birjand, Birjand, Iran


This study was conducted to determine the effect of drought stress on yield and some physiological characteristics of wheat cultivars. Six cultivars were grown under normal and drought stress in greenhouses and field conditions. Leaf samples were taken for physiological measurement including relative water content, transpiration rate, membrane stability, chlorophyll content and chlorophyll fluorescence parameters and stomatal frequency and length. Grain yield was determined for plants grown under field condition. Results showed that treatments have a significant impact on plant traits. Drought stress decreased leaf chlorophyll content and photochemical efficiency of PSII due to increasing F0 and decreasing Fm and increased ion leakage. Drought stress also decreased grain yield and the highest yield was obtained in plots with normal condition. Cultivars Alvand and Chamran showed the highest level of photochemical efficiency of PSII, membrane stability and grain yield under drought stress and were considered as the more tolerant cultivars to drought stress than other cultivars under conditions of this investigation.


Article Title [فارسی]

بررسی اثرات تنش خشکی بر ارقام مختلف گندم توسط برخی متغیرهای فیزیولوژیکی و عملکرد دانه

Authors [فارسی]

  • مهدیه عسکری 1
  • علی اکبر مقصودی مود 2
  • وحیدرضا صفاری 2
  • افسون عسگری 3
1 دانشجوی دکتری زراعت دانشگاه بیرجند، بیرجند و فارغ التحصیل کارشناسی ارشد از دانشگاه شهید باهنر کرمان، کرمان
2 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان،
3 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه بیرجند، بیرجند
Abstract [فارسی]

این تحقیق به منظور بررسی اثر تنش خشکی بر عملکرد و برخی خصوصیات فیزیولوژیک ارقام گندم انجام پذیرفت. شش رقم مورد مطالعه در شرایط گلخانه و مزرعه تحت تنش خشکی رشد یافتند. نمونه‌های برگی برای اندازه‌گیری خصوصیات فیزیولوژیک از قبیل طول روزنه سطوح بالایی و زیرین برگ، محتوی نسبی آب، سرعت تعرق، نشت یون، پایداری غشای کلروفیل و فلورسانس کلروفیل از گیاهان رشد یافته در شرایط گلخانه گرفته شدند. عملکرد دانه از گیاهان رشد یافته در شرایط مزرعه اندازه‌گیری گردید. نتایج نشان داد که تنش خشکی اثرات معنی­داری بر صفات اندازه‌گیری شده داشت. بین ارقام گندم نیز از نظر اکثر صفات مورد نظر اختلاف معنی­دار به دست آمد. تنش خشکی باعث افزایش F0 و کاهش Fm شد. همچنین تنش خشکی سبب کاهش عملکرد دانه گردید به طوری‌که بالاترین عملکرد از شرایط بدون تنش خشکی به‌دست آمد. ارقام الوند و چمران بالاترین شاخص کلروفیل، راندمان کوانتومی و عملکرد دانه را تحت شرایط تنش خشکی دارا بودند و به عنوان ارقام متحمل به تنش در این مطالعه شناسایی شدند.

Keywords [فارسی]

  • تنش خشکی
  • عملکرد
  • فلورسانس کلروفیل
  • کارایی فتوسیستمII
  • گندم
Baker NR and Rosenqvist E, 2004. Applications of chlorophyll fluorescence can improve crop production strategies: an examination of future possibilities. Journal of Experimental Botany 55: 1607-1621.
Baker NR, 2008. Chlorophyll fluorescence: a probe of photosynthesis in vivo. Annual Review of Plant Biology 59: 89-113.
Belkhodja R, Morales F, Abadia A, Go´mez-Aparisi J and Abadia J, 1994. Chlorophyll fluorescence as a possible tool for salinity tolerance screening in barley (Hordeum vulgare L.). Plant Physiology 104: 667-673.
Chaves MM, 1991. Effects of water deficits on carbon assimilation. Environmental and Experimental Botany 94: 33-45.
Cornic G, 2000. Drought stress inhibits photosynthesis by decreasing stomatal aperture-not by affecting ATP synthesis. Trends in Plant Science 5(5): 187-188.
Dai F, Zhou M and Zhang G, 2007. The change of chlorophyll fluorescence parameters in winter barley during recovery after freezing shock and as affected by cold acclimation and irradiance. Plant Physiology and Biochemistry 45: 915-921.
Demmig-Adams B and Adams III WW, 1992. Photo-protection in plants: a role for xanthophyll zeaxanthin. Annual Review of Plant Physiology and Plant Molecular Biology 1020: 1-24.
Everad JD, Gucci R, Kang SC, Flore JA and Leoscher WH, 1994. Gas exchange and carbon partitioning in the leaves of celery (Apium graviolens L.) at various levels of root zone salinity. Plant Physiology 106: 281-292.
Faraloni C, Cutinob I, Petruccelli R, Leva AR, Lazzeri S and Torzillo G, 2011. Chlorophyll fluorescence technique as a rapid tool for in vitro screening of olive cultivars (Olea europaea L.) tolerant to drought stress. Environmental and Experimental Botany 73: 49-56.
Fischer RA, Byerlee D and Edmeades GO, 2009. Can technology deliver on the yield challenge to 2050? In: FAO Expert Meeting on How to Feed the World in 2050, June 24-26, Rome.
Flexas J, Escalona JM, Evain S, Gul´ıas J, Moyam I, Osmond CB and Medrano H, 2002. Steady-state chlorophyll fluorescence (Fs) measurements as a tool to follow variation of net CO2 assimilation and stomatal conductance during water-stress in C3 plants. Physiologia Plantarum 114: 231-240.
Food and Agricultural Organization (FAO), 2012. FAOSTAT. Production; Crops. http://faostat.fao.org/site/567/default.aspx#ancor.
Galmés J, Medrano H and Flexa J, 2007. Photosynthetic limitation in response to water stress and recovery in Mediterranean plants with different growth forms. New Phytologist 175: 81-93.
Genty B, Briantais JM and Baker NR, 1989. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochimica et Biophysica Acta (BBA)  990: 87-92.
Gonzalez L and Gonzalez-Vilar M, 2003. Determination of relative water content. Handbook of Plant Ecophysiology Techniques. Kluwer Academic Publishers, London.
Govindjee A, 1995. Sixty-three years since Kautsky: chlorophyll a fluorescence. Australian Journal of Plant Physiology 22: 131-160.
Hoekstra FA, Golovina EA and Buitink J, 2001. Mechanisms of plant desiccation tolerance. Trends in Plant Science 6(9): 431-438.
Hu L, Wang Z, Du H and Huang B, 2009. Differential accumulation of dehydrins in response to water stress for hybrid and common Bermuda grass genotypes differing in drought tolerance. Journal of Plant Physiology 167: 103-109.
Krause GH and Weis E, 1991. Chlorophyll fluorescence and photosynthesis: the basics. Annual Review of Plant Physiology and Plant Molecular Biology 42: 313-349.
Lawlor DW and Cornic G, 2002. Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant, Cell and Environment. 25: 275-294.
Levitt J, 1980. Responses of Plants to Environmental Stresses. Water, Radiation, Salt and Other Stresses. Vol. II. Academic Press, New York.
Lichthenthaler HK, 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. In: Colowick SP and Kaplan NO (Eds.). Methods in Enzimology. Vol. 148. Pp. 350-382. Academic Press, San Diego, USA.
Lin M and Huybers P, 2012. Reckoning wheat yield trends. Environmental Research Letters 7(2): 024016. doi:10.1088/1748-9326/7/2/024016.
Maghsoudi-Mud AA, 2008. Physiological, morphological and anatomical basis of drought tolerance in wheat. Shahid Bahounar University Publication, Kerman, Iran.
Maxwell K and Johnson GN, 2000. Chlorophyll fluorescence a practical guide. Journal of Experiment Botany 51: 659-668.
Naumann JC, Young DR and Anderson JE, 2008. Leaf chlorophyll fluorescence, reflectance and physiological response to freshwater and saltwater flooding in the evergreen shrub, Myrica cerifera. Environmental and Experimental Botany 63: 402-409.
Nautiyal PC, Ravindra V and Joshi YC, 1995. Gas exchange and leaf water relations in two peanut cultivars of different drought tolerance. Biologia Plantarum 37: 371-374.
Nautiyal PC, NageswaraRao RC and Joshi YC, 2002. Moisture-deficit induced changes in leaf water content, leaf carbon exchange rate and biomass production in groundnut cultivators differing in specific leaf area. Field Crops Research 74: 67-79.
Ouzounidou G, 1993. Changes in variable chlorophyll fluorescence as a result of Cu-treatment: dose–response relations in Silene and Thlaspi. Photosynthetics 29: 455-462.
Percival GC and Sheriffs CN, 2002. Identification of drought-tolerant woody perennials using chlorophyll fluorescence. Journal of Arboriculture 28(5): 215-223.
Powles SB, 1984. Photoinhibition of photosynthesis induced by visible light. Annual Review of Plant Physiology 35: 15-44.
Quisenberry KS and Reitz LP, 1987. Wheat and Wheat Improvement. American Society of Agronomy Incorporation, Madison, WI, USA.
Richards RA, 2000. Selectable traits to increase crop photosynthesis and yield of grain crops. Journal of Experimental Botany 51: 447-458.
Rizza F, Pagani D, Stanca AM and Cattivelli L, 2001. Use of chlorophyll fluorescence to evaluate the cold acclimation and freezing tolerance of winter and spring oats. Plant Breeding 120: 389-396.
Sowinski P, Rudzin´ska-Langwald A, Adamczyk J, Kubica I and Fronk J, 2005. Recovery of maize seedling growth, development and photosynthetic efficiency after initial growth at low temperature. Journal of Plant Physiology 162: 67-80.
United Nations Population Division, 2000. World population prospects the 2000 revision highlights. Population Division, Department of Economic and Social Affairs, United Nations, NY, USA.
Woo NS, Badger MR and Pogson BJ, 2008. A rapid, non-invasive procedure for quantitative assessment of drought survival using chlorophyll fluorescence. Plant Methods 4: 27. doi: 10.1186/1746-4811-4-27.
Zarco-Tejada PJ, Miller JR, Mohammed GH, Noland TL and Sampson PH, 2002. Vegetation stress detection through chlorophyll a + b estimation and fluorescence effects on hyper spectral imagery. Journal of Environmental Quality 31(5): 1433-1441.