Assessment of some chlorophyll a fluorescence parameters of different corn cultivars in response to clodinafop-propagrgyl herbicide and salicylic acid

Document Type : Research Paper


1 Department of Plant Eco-Physiology, University of Tabriz, Tabriz, Iran

2 Department of Agronomy and Plant Breading, University of Tehran, Tehran, Iran


Nowadays, the use of chlorophyll a fluorescence has become a simple and nondestructive method for detecting plant’s photosynthetic response to different kinds of stress. The aim of this research was to evaluate the effects of different concentrations of clodinafop-propagrgyl (TOPIK®) herbicide on some chlorophyll a fluorescence parameters of different corn cultivars (SC260, SC400, SC704) in response to salicylic acid (SA) application (control, seed priming, spraying on plants three days before herbicide treatment, concurrent with herbicide application) in greenhouse of University of Tabriz in 2017, using a factorial arrangement of treatments based on randomized complete block design with three replicates. Results showed that by increasing of TOPIK levels in the absence of SA, the maximum fluorescence (Fm), variable fluorescence (Fv), efficiency and/or activity of water-splitting complex at donor side of photosystem II (Fv/F0) and maximum photochemical efficiency of photosystem II (Fv/Fm) decreased but minimum fluorescence (F0) increased. However, application of SA improved several parameters of chlorophyll a fluorescence in some corn cultivars. Application of SA as seed priming and spraying on plants three days before TOPIK treatment had higher effect on the studied cultivars than SA spraying concurrent with herbicide application. Correlation between the F0 and Fv/F0 was significant and negative. Also shoot dry weight (SDW) taken at 28 days after herbicide application had significant and positive relationship with Fv/Fm taken at 7 days after herbicide application. The findings of this research demonstrates that chlorophyll a fluorescence parameters and especially Fv/Fm are fast and reliable criteria for determining the effects of herbicides, such as TOPIK, and/or plants growth regulators, such as salicylic acid, shortly after treatment as compared to classical methods.


Article Title [فارسی]

ارزیابی برخی از پارامترهای فلورسانس کلروفیل a ارقام مختلف ذرت در پاسخ به علفکش کلودینافوپ- پروپارژیل و سالیسیلیک اسید

Authors [فارسی]

  • سیروس حسن نژاد 1
  • سهیلا پورحیدر غفاربی 2
1 گروه اکوفیزیولوژی گیاهی، دانشکده کشاورزی، دانشگاه تبریز
2 گروه زراعت و اصلاح نباتات، دانشکده علوم مهندسی زراعی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران
Abstract [فارسی]

امروزه استفاده از فلورسانس کلروفیل a به عنوان روشی ساده و غیرتخریبی برای تشخیص پاسخ‌ فتوسنتزی گیاهان به انواع تنش‌ها مطرح است. هدف این آزمایش ارزیابی اثر غلظت‌های مختلف علفکش کلودینافوپ-پروپارژیل (تاپیک) روی برخی پارامترهای فلورسانس کلروفیل a در ارقام مختلف ذرت (SC260، SC400، SC704) در پاسخ به کاربرد اسید سالیسیلیک (شاهد، پرایمینگ بذر، محلول‌پاشی گیاهان سه روز قبل از تیمار علفکش، محلول‌پاشی هم­زمان با علفکش) بود که در قالب یک آزمایش فاکتوریل بر پایه طرح بلوک­های کامل تصادفی در سه تکرار در گلخانه دانشگاه تبریز در سال 1396 انجام شد. نتایج نشان داد که با افزایش سطوح تاپیک، در غیاب اسید سالیسیلیک ، حداکثر فلورسانس (Fm)، فلورسانس متغیر (Fv)، کارایی و یا فعالیت کمپلکس تجزیه کننده آب در بخش دهنده الکترون فتوسیستم دو (Fv/F0) و حداکثر کارایی فتوشیمیایی فتوسیستم دو (Fv/Fm) کاهش یافت، ولی حداقل فلورسانس (F0) افزایش نشان داد. از طرف دیگر کاربرد اسید سالیسیلیک سبب بهبود تعدادی از پارامترهای فلورسانس کلروفیل a در برخی از ارقام ذرت شد. کاربرد اسید سالیسیلیک ، به صورت آماده‌سازی بذر و محلول‌ پاشی روی گیاهان، سه روز قبل از سمپاشی تاپیک، تأثیر بیشتری نسبت به محلول‌پاشی هم­زمان اسید سالیسیلیک با علفکش روی ارقام مورد مطالعه داشت. همبستگی بین F0 و Fv/F0 معنی‌دار و منفی بود. همچنین بین وزن خشک اندام هوایی در 28 روز بعد از سمپاشی و Fv/Fm در 7 روز بعد از سمپاشی، رابطه مثبت و معنی­داری وجود داشت. یافته­های آزمایش نشان داد که از پارامترهای مرتبط با فلورسانس کلروفیل a به ویژه Fv/Fm می‌توان به عنوان معیار­های سریع و قابل اطمینان به جای روش­های کلاسیک، برای ارزیابی اثرعلفکش­هایی مانند تاپیک و تنظیم‌کننده‌های رشد گیاهی مانند اسید سالیسیلیک، مدتی بعد از اعمال تیمار استفاده کرد.

Keywords [فارسی]

  • ذرت
  • اسید سالیسیلیک
  • فلورسانس کلروفیل a
  • کلودینافوپ - پروپارژیل
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