Impact of seed priming with different UV rays on morphological and physio-biochemical attributes of pea (Pisum sativum L.)

Document Type : Research Paper


1 Department of Plant Production and Genetics, Faculty of Agricultural Sciences and Engineering, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran.

2 Sararood Branch, Dryland Agricultural Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Kermanshah, Iran.

3 Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Maragheh, Iran.


In order to investigate the effect of priming and UV stress on pea (Pisum sativum L. cv. Dorian), a pot experiment was conducted as factorial based on completely randomized design with 22 treatments (11 type of seed priming and 2 UV stress conditions) and four replications at Razi University during 2014. The results showed that the impact of seed pre-treatment was significant on all traits studied. Also, effect of UV stress was significant on relative water content (RWC), maximum quantum yield of PSII, total chlorophyll (Chl total) content and hydrogen peroxide concentration (H2O2). Interaction between seed priming and UV stress was also significant (p≤ 0.01) for RWC, Chl total content and H2O2 concentration. Generally, the results indicated that UV stress has harmful effect on the pea plants. On the other hand, hydro-priming (HP) had a better effect on the morphological characteristics (stem length and fresh weight) and RWC, especially, under non-UV stress condition. But, HP for 12 h + UV-AB for 2 h and also HP for 11 h + UV-AB for 3 h showed the lowest Chl total content, maximum quantum yield of PSII, stem length and fresh weight of plant and also had the highest concentration of H2O2. Therefore, these two pre-treatments have a negative impact on the pea plant and their use is not recommended for the pre-treatment of seeds in pea.


Article Title [فارسی]

اثر پرایمینگ بذر با اشعه‌های مختلف فرابنفش بر پارامترهای مورفولوژیکی و فیزیولوژیکی-بیوشیمیایی نخود فرنگی (Pisum sativum L.)

Authors [فارسی]

  • سعید جلالی هنرمند 1
  • بهنوش رسایی 1
  • علی رسایی 2
  • مجید عبدلی 3
1 گروه مهندسی تولید و ژنتیک گیاهی، دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه رازی، کرمانشاه.
2 معاونت سرارود، مؤسسه تحقیقات کشاورزی دیم کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرمانشاه.
3 گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه مراغه، مراغه.
Abstract [فارسی]

به منظور ارزیابی تاثیر پرایمینگ و تنش فرابنفش بر رقم دورین نخود فرنگی (Pisum sativum L.)، یک آزمایش گلدانی به صورت فاکتوریل بر پایه طرح کاملا تصادفی با 22 تیمار (ترکیب 11 نوع پرایمینگ بذر و 2 شرایط تنش فرابنفش) در چهار تکرار در دانشگاه رازی طی سال 1394 به اجرا در آمد. نتایج نشان داد که تاثیر پیش تیمارهای بذر بر تمام صفات مورد مطالعه معنی­دار است. همچنین اثر تنش فرابنفش بر محتوای آب نسبی، حداکثر عملکرد کوانتومی فتوسیستم II، کلروفیل کل و غلظت پراکسید هیدروژن معنی­دار بود. اثر متقابل پرایمینگ بذر با تنش فرابنفش بر محتوای آب نسبی، کلروفیل کل و غلظت پراکسید هیدروژن در سطح احتمال یک درصد معنی­دار بود. به طور کلی نتایج نشان داد که تنش فرابنفش اثر مخربی بر روی گیاه نخود فرنگی می­گذارد. از سوی دیگر، هیدروپرایمینگ تاثیر بهتری بر صفات مورفولوژیکی (ارتفاع ساقه، وزن تر بوته) و فیزیولوژیکی (محتوای آب نسبی برگ) این گیاه، به ویژه در شرایط بدون تنش فرابنفش، داشت. اما ترکیب­های تیماری هیدروپرایمینگ به مدت 12 ساعت + UV-AB به مدت 2 ساعت و نیز هیدروپرایمینگ به مدت 11 ساعت + UV-AB به مدت 3 ساعت از کمترین میزان کلروفیل کل، حداکثر عملکرد کوانتومی فتوسیستم II، ارتفاع ساقه و وزن تر بوته و بیشترین غلظت پراکسید هیدروژن برخوردار شدند. در نتیجه، این پیش تیمارها تاثیر منفی بر گیاه نخود فرنگی دارند و استفاده از آن­ها برای پیش تیمار بذر نخود فرنگی توصیه نمی­شود. 

Keywords [فارسی]

  • اشعه­ های فرابنفش
  • پیش تیمار بذر
  • حداکثر عملکرد کوانتومی فتوسیستم II
  • نخود فرنگی (Pisum sativum L.)
  • محتوای آب نسبی
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