In Vitro Propagation of Lisianthus (Eustomagr andiflurom)

Document Type : Research Paper


1 MSc, Department of Agricultural Biotechnology, Tarbiat Modares University, Tehran, Iran

2 Department of Plant Breeding, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

3 Department of Agronomy and Plant Breeding, Faculty of Agriculture, Malayer University, Malayer, Iran


Nowadays, the most rapid method for producing healthy and disease-free Lisiantus is micropropagation. With respect to high economic value of this plant which is regarded among the 10 top cutting flowers in the world, this research was carried out to suggest a suitable protocol for its in vitro propagation, using nodal sections as an explant. To carry out this object, the effects of the pH (5.5, 5.6, 5.7, 5.8), culture vessel (small glass bottle, large glass bottle, polypropylene container), the concentration of macro elements, including NH4NO3 (1.45, 1.65, 1.85 g L-1), KNO3 (1.7, 1.9, 2.1 g L-1), CaCl2.2H2O (0.66, 0.44, 0.24 g L-1), MgSO4.7H2O (0.43, 0.37, 0.31 g L-1), KH2PO4 (0.13, 0.17, 0.21 g L-1), and the concentration of sucrose (25, 30, 35, 40 g L-1) were investigated in four independent experiments. The effects of the different studied factors were significant on the shoot regeneration. Results showed that pH 5.7 and the use of 35 g L-1 sucrose in MS medium were the best treatments for improving the number of shoots per explants (2.25 and 2 shoots, respectively). Moreover, increasing KH2PO4 concentration in MS medium produced the highest number of shoots per explant (3.5 shoots). The polypropylene container was also the best culture container for the lisianthus micropropagation (7.5 shoots per explant).


Article Title [فارسی]

بررسی ریزازدیادی گیاه لیسانتوس (Eustoma grandiflurom)

Authors [فارسی]

  • روح اله جعفری 1
  • احمد معینی 2
  • قاسم کریم زاده 2
  • زهرا موحدی 3
Abstract [فارسی]

لیسیانتوس (Eustoma grandiflorum) گیاه گلداری از خانواده Gentianaceae است که در بین ده گل شاخه بریده برتر دنیا قرار دارد. در حال حاضر، سریع‌ترین روش تکثیر لیسیانتوس برای تولید گیاهان سالم و عاری از بیماری، روش ریزازدیادی است. با توجه به ارزش بالای اقتصادی این گیاه، تحقیق حاضر جهت ارایه پروتکل مناسبی برای کشت درون شیشه‌ای آن، از ریز نمونه­های گرهی انجام شد. در این تحقیق، اثرات pH (5/5، 6/5، 7/5، 8/5)، ظرف کشت (ظرف شیشه‌ای کوچک، ظرف شیشه‌ای بزرگ و ظرف مکعبی از جنس پلی پروپیلن)، غلظت‌های مختلف نمک‌های پرمصرف شامل NH4NO3  (45/1، 65/1 و 85/1 گرم در لیتر)، KNO3 (7/1، 9/1 و 1/2 گرم در لیتر)، CaCl2.2H2O ، (24/0، 44/0 و 66/0 گرم در لیتر)،MgSO4.7H2O  (31/0، 37/0 و 43/0 گرم در لیتر)، KH2PO4  (13/0، 17/0 و 21/0 گرم در لیتر) و اثر غلظت‌های مختلف ساکارز (25، 30، 35 و 45 گرم در لیتر) در چهار آزمایش جداگانه مورد بررسی قرار گرفت. اثرات عوامل مختلف روی باززایی نوساقه معنی­دار بود. بیشترین تعداد نوساقه در هر گیاهچه از محیط کشت MS با pH  7/5 و  35 گرم در لیتر ساکارز به دست آمد. همچنین، افزایش غلظت NH4NO3 در محیط کشت بیشترین تعداد نوساقه به ازای هر ریزنمونه (5/3 نوساقه) را تولید کرد. ظروف مکعبی از جنس پلی پروپیلن نیز برای ریزازدیادی لیسیانتوس با تولید 5/7 نوساقه بهتر از سایر ظروف بودند.

Keywords [فارسی]

  • ظرف کشت
  • عناصر پرمصرف
  • pH
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