Effects of Drought Stress on Some Anatomical Characteristics of Barley Leaves

Document Type : Research Paper

Author

Assistant Professor, Department of Agriculture, Miandoab Branch, Islamic Azad University, Miandoab, Iran

Abstract


In this research, effect of drought stress on leaf characteristics was investigated in the experimental field of Miandoab Azad University, Iran using four facultative barley cultivars. Two separate experiments were conducted in pot and field conditions. In both experiments a factorial arrangement was used and the treatments were completely randomized in four replications. In each experiment half of the experimental units were drought stressed and the other half were irrigated normally. For the pot experiment, plants were sampled at fourth leaf stage in order to obtain winter leaves. For the field experiment, when plants reached at late stem elongation stage, penultimate leaves were sampled and used for preparing microscopic slides.
Xylem and phloem diameter and mesophyll, bundle sheath and epidermal cells area were measured in the transverse sections prepared from middle parts of the leaves. Significant differences were observed among genotypes under drought stress in terms of leaf characteristics. Results also showed that drought stress changes the diameter and the surface area of the cells. However, the changes were not the same in the winter and spring leaves. The changes in some cases such as diameter of xylem vessels were considerable. For example, in the cultivars Sahra and Jonob, the winter leaves had bigger xylem vessels under drought stress as compared to the normal condition while in the spring leaves the xylem diameter was smaller under the same condition.
 

Keywords

Article Title [Persian]

اثر تنش خشکی بر برخی خصوصیات آناتومیکی برگ های جو

Author [Persian]

  • فرشاد سرخی لله لو
گروه کشاورزی، واحد میاندوآب، دانشگاه آزاد اسلامی، میاندوآب
Abstract [Persian]

این تحقیق به منظور بررسی تاثیر تنش خشکی بر خصوصیات آناتومیکی برگ­های جو در دانشگاه آزاد اسلامی میاندوآب و با استفاده از چهار رقم جو صورت گرفت. دو آزمایش جداگانه در شرایط گلدانی و مزرعه­ای انجام شدند. در هر دو شرایط، آزمایش به صورت فاکتوریل پیاده شد و تیمارها به صورت کاملا تصادفی در چهار تکرار توزیع شدند. در هر آزمایش، نیمی از واحد­های آزمایشی تحت تأثیر تنش خشکی قرار گرفتند و نیمی دیگر به طور عادی آبیاری شدند. برای آزمایش گلدانی، گیاهان در مرحله چهار برگی برای به دست آوردن برگ­های زمستانی نمونه برداری شدند. برای آزمایش مزرعه­ای، زمانی که گیاهان در مرحله آخر افزایش طول ساقه بودند، برگ­های ماقبل آخر برای تهیه اسلاید­های میکروسکوپی مورد نمونه­برداری قرار گرفتند. قطر آوندهای چوبی و آبکشی و سطح سلول­های مزوفیلی، غلاف آوندی و اپیدرمی از قسمت میانی برگ­های ارقام مورد آزمایش اندازه­گیری شدند. نتایج نشان داد که اختلاف معنی­داری بین ژنوتیپ­ها در شرایط تنش خشکی از نظر ویژگی­های برگی وجود دارد. بر اساس نتایج حاصل، تنش خشکی قطر آوندها و سطح سلول­ها­ را تغییر داد و این تغییرات در برگ­های زمستانه و بهاره یکسان نبودند. میزان تغییرات در برخی از خصوصیات اندازه­گیری شده مانند قطر آوندی قابل توجه بود. برای نمونه در ارقام صحرا و جنوب، برگ­های زمستانه تحت تنش خشکی نسبت به برگ­های شرایط عدم تنش خشکی دارای قطر آوند چوبی بیشتری بودند در حالی که برگ­های بهاره از قطر آوندی کمتری تحت شرایط مشابه برخوردار بودند.
 

 
 

Keywords [Persian]

  • برگ
  • جو
  • خشکی
  • خصوصیات آناتومیکی

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Journal of Plant Physiology and Breeding
Volume 7, Issue 2
December 2017
Pages 11-21

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