Physio-biochemical and Enzymatic Responses of Sunflower to Drought Stress

Document Type : Research Paper

Authors

1 Department of Biology, Faculty of Science, Urmia University, Urmia, Iran

2 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran

3 Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran

Abstract

Drought stress is a serious adverse factor that limits sunflower growth and productivity. The stress induces a range of physiological and biochemical responses in sunflower. So, evaluation of defense systems is important for producing resistant cultivars. In this study, physio-biochemical changes and antioxidant enzymes activitiesof six sunflower lines were evaluated under normal and irrigation at 60 and 40% of the field capacity using a randomized complete block design with three replications. Different characteristics such as relative water content (RWC), chlorophyll a and b contents, carotenoid and proline contents, lipids peroxidation and accumulation of malon dialdehyde (MDA), as well as activity of antioxidative enzymes like guaiacol peroxidase (GPX), ascorbate peroxidase (APX), catalase (CAT) and glutathione reductase (GR) were studied. The results showed significant differences among sunflower lines for physio-biochemical and enzymes activity under drought stress. According to the results, the lines C104 and RHA266 showed the better tolerance to drought stress. The founding of this study can be useful in sunflower breeding programs for producing resistant cultivars to drought stress.
 

Keywords


Article Title [فارسی]

پاسخ‌های فیزیوبیوشیمیایی و آنزیمی آفتابگردان به تنش خشکی

Abstract [فارسی]

تنش خشکی یک عامل مخرب جدی به شمار می­رود و باعث کاهش رشد و عملکرد آفتابگردان می­شود. خشکی سبب ایجاد پاسخ­های فیزیولوژیک و بیوشیمیایی در آفتابگردان می­گردد. از این رو، بررسی سیستم دفاعی این گیاه جهت تولید ارقام مقاوم حائز اهمیت است. در پژوهش حاضر تغییرات فیزیوبیوشیمیایی و فعالیت آنزیم­های آنتی اکسیدانی در شش لاین آفتابگردان تحت شرایط نرمال و آبیاری 60 و 40 درصد ظرفیت زراعی در قالب طرح بلوک­های کامل تصادفی در سه تکرار ارزیابی شد. به این منظور خصوصیاتی از قبیل محتوای آب نسبی، میزان کلروفیل­های a,b، میزان کاروتنوئید و پرولین، پراکسیداسیون لیپیدها و تجمع مالون دی آلدئید، فعالیت آنزیم­های آنتی اکسیدانی مانند گایاکول پراکسیداز، آسکوربات پراکسیداز، کاتالاز و گلوتاتیون ردوکتاز اندازه­گیری شد. نتایج نشان داد که تفاوت معنی­داری در بین لاین­های مورد مطالعه برای خصوصیات فیزیوبیوشیمیایی و فعالیت آنزیمی تحت شرایط تنش خشکی وجود دارد . لاین­های C104 و RHA266 مقاومت خوبی به تنش خشکی نشان دادند. نتایج حاصل از این پژوهش می­تواند در برنامه­های به نژادی آفتابگردان جهت تولید ارقام مقاوم به خشکی مفید باشد.
 

Keywords [فارسی]

  • آفتابگردان
  • پراکسیداسیون چربی­ها
  • تحمل به خشکی
  • خصوصیات فیزیولوژیک
  • فعالیت آنزیمی
Acar O, Turkan I and Zdemir FO, 2001.Superoxide dismutase and peroxidase activities in drought sensitive and resistant barley (Hordeum vulgare L.) varieties. Acta Physiologiae Plantarum3: 351-356.
Alaei Y, 2011. The effect of amino acids on leaf chlorophyll content in bread wheat genotypes under drought stress conditions. Middle-East Journal of Scientific Research 10: 99-101.
Andrade A, Vigliocco A, Alemano S, Llanes A and Abdala G, 2013. Comparative morpho-biochemical responses of sunflower lines sensitive and tolerant to water stress. American Journal of Plant Sciences 4: 156-167.
Anjum SH, Xie XY, Wang LC, Saleem MF, Man C and Lei W, 2011. Morphological, physiological and biochemical responses of plants to drought stress. African Journal of Agricultural Research 6: 2026-2032.
Antolin MC and Sanchez-Diaz M, 1993. Effect of temporary drought on photosynthesis of alfalfa plants. Journal of Experimental Botany 44: 1341-1349.
Asada RD, 1992. Ascorbate peroxidas-a hydrogen peroxide scavenging enzyme in plants. Plant Physiology 85: 235-241.
Baldini M and Vannozzi GP, 1998. Agronomic and physiological assessment of genotypic variation for drought tolerance in sunflower genotypes obtained from a cross between H. annuus and H. argophyllus. Agricultural Medicine 128: 232-240.
Baldini M, Vannozzi GP, Gomez Sanchez D and Turi M, 1997. Results of a physiological selection approach to improve drought resistance in sunflower. Genetika-a-Slechteni 33: 181-195.
Barraclough PB and Kyte J, 2001. Effect of water stress on chlorophyll meter reading in wheat. Plant Nutrition 92: 722-23.
Bartoli CG, Simontacchi M, Tambussi E, Beltrano J, Montaldi E and Puntarulo S, 1999. Drought and watering-dependent oxidative stress. Effect on antioxidant content in Triticm aestivum L. leaves. Journal of Experimental Botany 332: 375-383.
Bastide B, Deborah S, Janet H and Lrwin T, 1933. Effects of severe water stress on aspects of crassulacean acid metabolism in Xerosicyos dangui. Plant Physiology 103: 1089-1096.
Bates LS, Walderon RP and Teare ID, 1973. Rapid determination of free proline for water stress studies. Plant and Soil 39: 205-208.
Beard JB, 1973. Turf Grass: Science and Culture. Prentice-Hall, Englewood Cliffs, NJ, USA.
Bor M, Ozdemir F and Turkan I, 2003. The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Betamaritima L. Plant Science 164: 77-84.
Cao HX, Sun CX, Shao HB and Lei XT, 2011. Effects of low temperature and drought on the physiological and growth changes in oil palm seedlings. African Journal of Biotechnology 10: 2630–2637.
Cellier F, Conejero G, Breitler JC and Casse F, 1998. Molecular and physiological responses to water deficit in drought-tolerant and drought-sensitive lines of sunflower: accumulation of dehydrin transcripts correlates with tolerance. Plant Physiology 116: 319-328.
Chachar MH, Chachar NA, Chachar Q, Mujtaba SM, Chachar S and Chachar Z, 2016. Physiological characterization of six wheat genotypes for drought tolerance. International Journal of Research-Granthaalayah 4: 184-196.
Chang CJ and Kao CH, 1998. H2O2 metabolism during senescence of rice leaves: change in enzyme activities in light and darkness. Plant Growth Regulation 25: 11-15.
Chavan PD and Karadge BA, 1986. Growth, mineral nutrition, organic constituents and rate of photosynthesis in Sesbania grandiflora L. grown under saline conditions. Plant and Soil 93: 395-404.
Chen C and DickmanMB, 2005. Proline suppresses apoptosis in the fungal pathogen Colletotrichum trifolli. Proceedings of the National Academy of Sciences 102: 3459-3464.
Chen THH and Murata N, 2000. Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes. Current Opinion in Plant Biology 5: 250-257.
Darvishzadeh R, Pirzad A, Hatami Maleki H, Poormohammad Kiani S and Sarrafi A, 2010. Evaluation of the reaction of sunflower inbred lines and their F1 hybrids to drought conditions using various stress tolerance indices. Spanish Journal of Agricultural Research 8: 1037-1046.
Davar R, Darvishzadeh R and Majd A, 2013. Changes in antioxidant systems in sunflower partial resistant and susceptible lines as affected by Sclerotinia sclerotiorum (Lib.) de Bary. Biologia 68: 821-829.
Duan B, Yang Y, Lu Y, Korpelainen H, Berninger F and Li C, 2007. Interactions between drought stress, ABA and genotypes in Picea asperata. Journal of Experimental Botany 58: 3025-3036.
Errabii T, Gandonou CB, Essalmani H, Abrini J, Idaomar M and Skalisenhaji N, 2006. Growth, proline and ion accumulation in sugarcane callus cultures under drought induced osmotic stress and its subsequent relief. African Journal of Biotechnology 5: 1488-1493.
Farooq M, Wahid A, Kobayashi N, Fujita D and Basra SMA, 2009. Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development 29: 185-212.
Ghobadi M, Shayesteh Taherabadi SH, Ghobadi ME, Mohammadi GR and Honarman SJ, 2013. Antioxidant capacity, photosynthetic characteristics and water relations of sunflower (Helianthus annuus L.) cultivars in response to drought stress. Industrial Crops and Products 50: 29-38.
Gill SS and Tuteja N, 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48: 909-930.
Groden D and Beck E, 1979. H2O2 destruction by ascorbate-dependent system from chloroplast. Biochimica et Biophysica Acta 546: 426-435.
Guo Z, Ou W, Lu S and Zhong Q, 2006. Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiology and Biochemistry44: 828-836.
Hare PD and CressWA, 1997. Metabolic implications of stress induced proline accumulation in plants. Plant Growth Regulation 21: 79-102.
Heath RL and Packer L, 1968. Photoperoxidation in isolated chloroplasts. Archives of Biochemistry and Biophysics 125: 850-857.
Hussain S, Saleem MF, Iqbal J, Ibrahim M, Ahmad M, Nadeem SM, Ali A and Atta S, 2015. Abscisic acid mediated biochemical changes in sunflower (Helianthus annuus L.) grown under drought and well-watered field conditions. The Journal of Animal and Plant Sciences 25: 406-416.
Hussain S, Saleem MF, Iqbal J, Ibrahim M, Atta S, Ahmed T and Rehmani MIA, 2014. Exogenous application of abscisic acid may improve the growth and yield of sunflower hybrids under drought. Pakistan Journal of Agricultural Sciences 51: 49-58.
Jabari F, Ahmadi A, Poustini K, Alizadeh H, Sharifzadeh F and Ranjbar M, 2009. Evaluation of relationship between relative water content and gas exchanges parameters with drought resistance in 7 wheat cultivars. Iranian Journal of Field Crop Science 40: 198-207 (In Persian with the English abstract).
Jiang Y and Huang N, 2001. Drought and heat stress injury to two cool-season turf grass in relation to antioxidant metabolism and lipid peroxidation. Crop Science 41: 436-442.
Jung S, 2004. Variation in antioxidant metabolism of young and mature leaves of Arabidopsis thaliana subjected to drought plant. Plant Sciences 166: 459-466.
Karuppanapandian T, Moon JH, Kim C, Manoharan, K and Kim W, 2011. Reactive oxygen species in plants: their generation, signal transduction and scavenging mechanisms. Australian Journal of Crop Science 5: 709-725.
Khanna Chopra R and Selote DS, 2007. Acclimation to drought stress generates oxidative stress tolerance in drought resistant than susceptible wheat cultivar under field conditions. Environmental and Experimental Botany 60: 276-283.
Khayatnezhad M, Gholamin RS, Jamaatie Somarin H and Zabihie Mahmoodabad R, 2011. The leaf chlorophyll content and stress resistance relationship considering in corn cultivars (Zea mays L.). Advances in Environmental Biology 5: 118-122.
Kim KS, Beard JB and Sifers SI, 1989. Drought resistance comparisons among major warm-season turf grasses. USGA Green Section Record 26(5): 12-15.
Levitt J, 1980. Response of Plants to Environmental Stresses: Water, Radiation, Salt and Other Stresses. Academic Press, New York.
Lichtenthaler HK and WellburnAR, 1985. Determination of total carotenoids and chlorophylls a and b of leaf in different solvents. Biochemical Society Transactions 11: 591-592.
Lopez-Berenguer C, Martinez Ballesta MC, Moreno DA, Carvajal M and Garcia Viguera C, 2009. Growing hardier crops for better health: salinity tolerance and the nutritional value of broccoli. Journal of Agricultural and Food Chemistry 57: 572-578.
Maehly AC and Chance B, 1959. The assay of catalase and peroxidase. In: Click D (Eds). Method of Biochemical Analysis. Pp. 357-425. Inderscience Publishers, New York.
Manivannan P, Jaleel CA, Sankar B, Kishorekumar A, Somasundaram R, Alagu Lakshmanan GM and Panneerselvam R, 2007. Growth, biochemical modifications and proline metabolism in Helianthus annuus L. as induced by drought stress. Colloids and Surfaces B: Biointerfaces 59: 141-149.
Maury P, Berger M, Mojayad F and Planchon C, 2000. Leaf water characteristics and drought acclimation in sunflower genotypes. Plant and Soil 223: 153-160.
Monakhova OF and Chernyadev II, 2002. Protective role of kartolin-4 in wheat plants exposed to soil drought. Applied and Environmental Microbiology 38: 373-380.
Moustafa MA, Boersma L and Kronstad WE, 1996. Response of four spring wheat cultivars to drought stress. Crop Science 36: 982-986.
Munne Bosch S and Alegre L, 2000. Changes in carotenoids, tocopherols and diterpenes during drought and recovery, and the biological significance of chlorophyll loss in Rosmarinus officinalis plants. Planta 210: 925-931.
Naik CR and Joshi CL, 1983. Ineffectual role of proline metabolism in salt stressed sugarcane leaves. Proceedings of the IndianAcademy of Science 92: 265-269.
Nayyar H and Gupta D, 2006. Differential sensitivity of C3 and C4 plant to water deficit stress: association with oxidative stress and antioxidants. Environmental and Experimental Botany 58: 106-113.
Noctor G and Foyer C, 1998. Ascorbate and glutathione: keeping active oxygen under control. Plant Physiology 49: 249-279.
Pasda G and Diepenbrock W, 1990. The physiological yield analysis of sunflower. Part II. Climate factors. Wissenschfat Technologie 93: 155-168.
Poormohammad Kiani S, Grieu P, Maury P, Hewezi T, Gentzbittel L and Sarrafi A, 2007. Genetic variability for physiological traits under drought conditions and differential expression of water stress-associated genes in sunflower (Helianthus annuus L.). Theoretical and Applied Genetics 114: 193-207.
Poormohammad Kiani S, Maury P, Sarrafi A and Grieu P, 2008. QTL analysis of chlorophyll fluorescence parameters in sunflower (Helianthus annuus L.) under well-watered and water-stressed conditions. Plant Science 175: 565-573.
Poormohammad Kiani S, Nouri L, Maury P, Darvishzadeh R, Grieu P and Sarrafi A, 2009. Genetic variation and identification of molecular markers associated with osmotic adjustment-related traits in gamma irradiation-induced mutants of sunflower (Helianthus annuus L.). Journal of Genetics and Breeding 62: 67-74.
Rampino P, Pataleo S, Gerardi C, Mita G and Perrotta C, 2006. Drought stress response in wheat: physiological and molecular analysis of resistant and sensitive genotypes. Plant Cell and Environment 29: 2143-2152.
Rao KVM, 2006. Introduction. In: Rao KVM, RaghavendraAS and Reddy KJ (Eds). Physiology and Molecular Biology of Stress Tolerance in Plants. Pp.1-14. Springer, The Netherlands.
Rauf S and Sadaqat HA, 2008. Identification of physiological traits and genotypes combined to high achene yield in sunflower (Helianthus annuus L.) under contrasting water regimes. Australian Journal of Crop Science 1: 23-30.
Reddy AR, Chaitanya KV and Vivekanandan M, 2004. Drought induced responses of photosynthesis and antioxidant metabolism in higher plants. Journal of Plant Physiology 161: 1189-1202.
Safavi SM, SafaviAS and Safavi SA, 2015. Evaluation of drought tolerance in sunflower (Helianthus annuus L.) inbred lines and synthetic varieties under non Stress and drought stress conditions. Biological Forum l 7: 1849-1854.
Sairam R, Rao K and Srivastava C, 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science163: 1037-1046.
Sairam RK and Srivastava C, 2001. Water stress tolerance of wheat (Triticum aestivum L): variations in hydrogen peroxide accumulation and antioxidant activity in tolerant and susceptible genotypes. Journal of Agronomy and Crop Science 186: 63-70.
Schurr U, Heckenberger U, Herdel K, Walter A and Feil R, 2000. Leaf development in Ricinus communis during drought stress: dynamics of growth processes of cellular structure and of sink-source transition. Journal of Experimental Botany 51: 1515-1529.
Sharma P and Dubaey RS, 2005. Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedling. Plant Growth Regulation 46: 209-221.
Slama I, Messedi D, Ghnaya T, Savoure A and Abdelly C, 2006. Effects of water deficit on growth and proline metabolism in Sesuvium portulacastrum. Environmental and Experimental Botany 56: 231-238.
Smirnoff N, 1993. The role of active oxygen in the response of plant to water deficit and desiccation. New Phytologist 125: 27-58.
Tahir MHN, Muhammad I and Hussain MK, 2002. Evaluation of sunflower (Helianthus annuus L.) inbred lines for drought tolerance. International Journal of Agriculture and Biology 4: 398-400.
Terbea M, Vranceanu AV, Petcu E, Craiciu DS and Micut G, 1995. Physiological response of sunflower plants to drought. Romanian Agricultural Research 3: 61-67.
Vinocur B and Altman A, 2005. Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations. Current Opinion in Biotechnology 16: 123-132.
Wang JR, Li SX and Li KI, 2001. Effect of water limited deficit stress during different growth stages on leaf enzymes of winter wheat. Acta Botanica Boreali-Occidentalia Sinica 21: 47-52.
Wang L, Liu D, Ahmed T, Chung FL, Conaway C and Chiao JW, 2004. Targeting cell cycle machinery as a molecular mechanism of sulforaphane in prostate cancer prevention. International Journal of Oncology 24: 187-192.
Zhang J, Cui SLIJ and KirkhamMB, 1995. Protoplasmic factors, antioxidant responses, and chilling resistance in maize. Plant Physiology and Biochemistry 33: 567-575.