Physio-biochemical and Enzymatic Responses of Sunflower to Drought Stress

Document Type : Research Paper


1 Department of Biology, Faculty of Science, Urmia University, Urmia, Iran

2 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran

3 Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran


Drought stress is a serious adverse factor that limits sunflower growth and productivity. The stress induces a range of physiological and biochemical responses in sunflower. So, evaluation of defense systems is important for producing resistant cultivars. In this study, physio-biochemical changes and antioxidant enzymes activitiesof six sunflower lines were evaluated under normal and irrigation at 60 and 40% of the field capacity using a randomized complete block design with three replications. Different characteristics such as relative water content (RWC), chlorophyll a and b contents, carotenoid and proline contents, lipids peroxidation and accumulation of malon dialdehyde (MDA), as well as activity of antioxidative enzymes like guaiacol peroxidase (GPX), ascorbate peroxidase (APX), catalase (CAT) and glutathione reductase (GR) were studied. The results showed significant differences among sunflower lines for physio-biochemical and enzymes activity under drought stress. According to the results, the lines C104 and RHA266 showed the better tolerance to drought stress. The founding of this study can be useful in sunflower breeding programs for producing resistant cultivars to drought stress.


Article Title [Persian]

پاسخ‌های فیزیوبیوشیمیایی و آنزیمی آفتابگردان به تنش خشکی

Abstract [Persian]

تنش خشکی یک عامل مخرب جدی به شمار می­رود و باعث کاهش رشد و عملکرد آفتابگردان می­شود. خشکی سبب ایجاد پاسخ­های فیزیولوژیک و بیوشیمیایی در آفتابگردان می­گردد. از این رو، بررسی سیستم دفاعی این گیاه جهت تولید ارقام مقاوم حائز اهمیت است. در پژوهش حاضر تغییرات فیزیوبیوشیمیایی و فعالیت آنزیم­های آنتی اکسیدانی در شش لاین آفتابگردان تحت شرایط نرمال و آبیاری 60 و 40 درصد ظرفیت زراعی در قالب طرح بلوک­های کامل تصادفی در سه تکرار ارزیابی شد. به این منظور خصوصیاتی از قبیل محتوای آب نسبی، میزان کلروفیل­های a,b، میزان کاروتنوئید و پرولین، پراکسیداسیون لیپیدها و تجمع مالون دی آلدئید، فعالیت آنزیم­های آنتی اکسیدانی مانند گایاکول پراکسیداز، آسکوربات پراکسیداز، کاتالاز و گلوتاتیون ردوکتاز اندازه­گیری شد. نتایج نشان داد که تفاوت معنی­داری در بین لاین­های مورد مطالعه برای خصوصیات فیزیوبیوشیمیایی و فعالیت آنزیمی تحت شرایط تنش خشکی وجود دارد . لاین­های C104 و RHA266 مقاومت خوبی به تنش خشکی نشان دادند. نتایج حاصل از این پژوهش می­تواند در برنامه­های به نژادی آفتابگردان جهت تولید ارقام مقاوم به خشکی مفید باشد.

Keywords [Persian]

  • آفتابگردان
  • پراکسیداسیون چربی­ها
  • تحمل به خشکی
  • خصوصیات فیزیولوژیک
  • فعالیت آنزیمی
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