Trichoderma-Induced Enhancement of Soybean Seedling Performance in Response to Salt Stress

Document Type : Research Paper


1 Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran

2 Department of Plant Protection, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran


In this experiment soybean seeds were pre-treated with salt tolerant isolate of Trichoderma harzianum to evaluate the different aspects of seedling growth and metabolism in response to different concentrations of NaCl. Trichoderma isolate was more effective in improving dry weight and root volume of seedlings during mild salt stress. Seedlings obtained from bioprimed seeds had significantly higher leaf greenness, chlorophyll fluorescence, net photosynthesis and stomatal conductance than the control at all stress levels. NaCl-induced membrane damage was alleviated by Trichoderma, especially at 3 dS/m. The biopriming treatment showed lower accumulation of malondialdehyde (MDA) content under saline condition. Highest MDA content was recorded in the control (unprimed) seeds at salinity level of 9 dS/m. A common factor that adversely affects plants under saline conditions is generation of reactive oxygen species (ROS) and we tested the hypothesis that seed biopriming alleviated damages resulting from ROS attack in the stressed plants. Greatest catalase activity was detected in the bioprimed seeds at the salt stress level of 9 dS/m. The activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX) were significantly increased in response to NaCl stress. Seed biopriming enhanced SOD and APX activity averaged over all salinity levels. It could be concluded that seed biopriming with Trichoderma harzianum certainly ameliorated harmful impacts of mild salinity mainly through promotion of early seedling development and antioxidative defence system.


Article Title [Persian]

افزایش رشد ناشی از تریکودرما در گیاهچه سویا در پاسخ به تنش شوری

Abstract [Persian]

به منظور ارزیابی جنبه­های مختلف رشد و متابولیسم گیاهچه در پاسخ به غلظت­های مختلف NaCl، بذور سویا طی یک آزمایش گلدانی با جدایه متحمل به شوری Trichoderma harzianum پیش تیمار شدند. جدایه تریکودرما در بهبود وزن خشک و حجم ریشه گیاهچه­ها در شرایط شوری ملایم سودمندتر بود. گیاهچه­های حاصل از توده بذری بیو­پرایم شده به طور معنی­داری از سبزینگی برگ، فلورسانس کلروفیل، فتوسنتز خالص و هدایت روزنه­ای بالاتری در همه سطوح تنش شوری در مقایسه با شاهد برخوردار بود. آسیب ناشی از نمک به غشای سلولی به ویژه در شوری 3 dS/m  از طریق کاربرد تریکودرما جبران شد. تیمار بیوپرایمینگ تجمع مالون دی­آلدهید پایین­تری در شرایط شوری نشان داد. بالاترین مقدار مالون دی­آلدهید در بذور پرایم نشده در سطح شوری 9 dS/m  ثبت شد. عامل شایعی که به طور منفی گیاهان را تحت شرایط شور متاثر می­سازد تولید انواع اکسیژن واکنشی (ROS) است. در این پژوهش فرضیه­ای مبنی بر اثر ترمیمی بیوپرایمینگ بذر بر هجوم ROS در گیاهان تحت تنش مورد آزمون قرار گرفت. بالاترین فعالیت آنزیم کاتالاز در توده بذری بیوپرایم شده در سطح شوری 9 dS/m شناسایی شد. فعالیت سوپراکسید دیسموتاز (SOD) و آسکوربات پراکسیداز (APX) به طور معنی­داری در پاسخ به تنش شوری افزایش یافت. بیوپرایمینگ بذر فعالیت آنزیم­های SOD و APX را در متوسط سطوح شوری افزایش داد. به طور کلی می­توان نتیجه گرفت که به احتمال زیاد بیوپرایمینگ بذر با Trichoderma harzianum اثرات زیان بار تنش ملایم شوری را عمدتا به واسطه تحریک نمو اولیه و سیستم دفاعی آنتی­اکسیدان گیاهچه­ها مرتفع می­سازد.

Keywords [Persian]

  • آنزیم­های آنتی اکسیدان
  • فتوسنتز
  • قدرت گیاهچه
  • Glycine max
  • NaCl
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