Effects of Priming with Salicylic Acid on Safflower Seedlings Photosynthesis and Related Physiological Parameters

Document Type : Research Paper


Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Zanjan, Iran


Generally, primed seeds produce larger and heavier plants than non-primed seeds. This may be simply due to rapid emergence and extension of leaf growth, or the influence of other physiological processes. The effect of seed pretreatment with salicylic acid (SA) on some physiological and photosynthetic characteristics of the safflower seedlings, cv. Goldasht, was examined under field condition. The treatments were different levels of SA (including zero or hydropriming, 400, 800, 1200, 1600, 2000 and 2400 µM and a treatment of the non-primed seeds as the control). Priming enhanced photosynthesis rate (PN) chlorophyll content index (CCI), relative water content (RWC) and seedling dry weight. Also, primed seeds had higher stomatal conductance (gs) and transpiration rate (E) than the control and the hydroprimed treatments. The lowest intercellular CO2 (Ci) and highest cell membrane stability was obtained in 2400 µM SA. But values of the control treatment were opposite. In addition, highest carboxylation efficiency (CE) and photosynthetic water use efficiency (WUEb) were found in 2000 and 2400 µM of SA. It seems seed priming with SA, increased gs and hence PN by improving RWC status. This was associated with enhanced WUE and CE at higher levels of SA. A positive relationship was found between WUEb and PN, CE and RWC but negative relationship with Ci. It seems that increase in plants dry weight by priming not only was the result of rapid growth rate, but also the enhancement of PN, RWC and chlorophyll content.


Article Title [Persian]

اثر پرایمینگ بذر با اسید سالیسلیک بر فتوسنتز و خصوصیات فیزیولوژیکی مرتبط در گیاهچه‌های گلرنگ

Abstract [Persian]

 به طور معمول، بذرهای پرایم شده گیاهان درشت‏تر و سنگین‏تری را در مقایسه با بذرهای پرایم نشده تولید می‏کنند. این عمل ممکن است به سادگی به دلیل افزایش سرعت سبز کردن و توسعه سطح برگ و یا به دلیل سایر فرایندهای فیزیولوژیک صورت گیرد. در این پژوهش اثر پیش‏تیمار بذر با اسید سالیسیلیک بر برخی خصوصیات فیزیولوژیک و فتوسنتزی گیاهچه‏های گلرنگ، رقم گلدشت، تحت شرایط مزرعه مورد ارزیابی قرار گرفت. تیمارها سطوح مختلف اسید سالیسیلیک (شامل صفر یا هیدروپرایمینگ، 400، 800، 1200، 1600، 2000 و 2400 میکرو مولار و یک تیمار از بذرهای تیمار نشده به عنوان شاهد) بودند. در بین تیمارها حداکثر سرعت فتوسنتز، شاخص محتوای کلروفیل، محتوای نسبی آب و حداکثر ماده خشک در تیمار 2400 میکرو مولار مشاهده شد. در تیمار پرایمینگ گیاهان دارای هدایت روزنه‏ای و سرعت تعرق بالاتری نسبت به گیاهان شاهد بودند. کمترین غلظت دی اکسید کربن زیر اتاقک روزنه در تیمار 2400 و بیشترین در تیمار شاهد مشاهده گردید. کمترین نشت الکترولیت، بیشترین کارایی کربوکسیلاسیون و کارایی مصرف آب فتوسنتزی در تیمارهای 2000 و 2400 میکرو مولار به دست آمد. به نظر می‏رسد پرایمینگ بذر با اسید سالیسیلیک از طریق افزایش محتوای نسبی آب موجب افزایش هدایت روزنه و در نتیجه سرعت فتوسنتز شده است. این عمل در سطوح بالای اسید سالیسیلیک موجب افزایش کارایی مصرف آب و کارایی کربوکسیلاسیون شد. کارایی مصرف آب فتوسنتزی رابطه مثبتی با سرعت فتوسنتز، کارایی کربوکسیلاسیون و محتوای نسبی آب داشت، ولی رابطه آن با غلظت دی کربن اکسید زیر اتاقک روزنه منفی بود. به طور کلی، اسید سالیسیلیک در گیاهان تیمار شده ماده خشک را از طریق بهبود محتوای کلروفیل، محتوای نسبی آب و سرعت فتوسنتز افزایش داد.

Keywords [Persian]

  • اسید سالیسیلیک
  • فتوسنتز
  • کارایی کربوکسیلاسیون
  • کارایی مصرف آب
  • محتوای نسبی آب
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