Vascular Architecture Alterations in Expanding Durum Wheat Leaf Under Salinity

Document Type : Research Paper


1 1Department of Agroecology, Agriculture College and Natural Resources of Darab, Shiraz University, Iran

2 Department of Crop Production and Plant Breeding, College of Agriculture, Shiraz University, Shiraz, Iran


The leaf growth and cross-sectional area of durum wheat during its development may be reduced under salt stress due to vascular architecture alterations of leaves. A hydroponic experiment was conducted to compare growth rate and vascular architecture changes of two durum wheat cultivars including Shabrang and Yavaroos under 0 and 100 mM NaCl. Plants were sampled at the three-leaf stage growth. Results showed that under salt stress at 15 DAG, Shabrang with 0.69 mm/h had a greater elongation rate than Yavaroos (0.27 mm/hr). Likewise, under salt stress at 25 mm above the leaf base, 32 and 37% reductions in cross sectional area were observed in Shabrang and Yavaroos, respectively. In all treatments, maximum leaf width was obtained at the growth zone (25 mm above the leaf base). In both cultivars, the leaf cross-section of the control consisted of one midrib, 5 large veins and 11-21 small veins, while it composed of one midrib, 4 large veins and 3-12 small veins under salt stress. Overall, in both cultivars, comparison of control and salt stress treatments showed that the reduction in protoxylem area at 5 mm was greater than 100 mm above the leaf base. It can be concluded that the reduction in the cross-section of durum wheat is mainly correlated with a decreased number of small veins, and Shabrang cultivar with greater number and area of small veins along the leaf base had higher leaf growth and expansion rate than Yavaroos, when plants exposed to salt stress. This probably can explain why Shabrang cultivar might be more tolerant to salt stress than Yavaroos.


Alavi Matin SM, Rahnama A and Meskarbashi M, 2015. Effects of type and rate of potassium fertilizer on agronomic and physiological traits of two durum wheat varieties under salt stress. Cereal Research 5: 177-187.
Baum SF, Tran PN and Silk WK, 2000. Effects of salinity on xylem structure and water use in growing leaves of sorghum. New Phytology 146: 119–127.
Bernstein N, Silk WK and Lauch A, 1993. Growth and development of sorghum leaves under conditions of NaCl stress: spatial and temporal aspects of leaf growth inhibition. Planta 191: 433–439.
Bijanzadeh E, and Emam Y, 2015. Effect of salt stress on root anatomy and hydraulic conductivity of barley cultivars. Iran Agricultural Research 34: 71-79.
Bijanzadeh E and Kazemeini SA, 2014. Tissue architecture changes of expanding barley (Hordeum vulgare L.) leaf under salt stress. Australian Journal of Crop Science 8: 1373-1379.
Brundrett MC, Enstone DE and Peterson CA, 1988. A berberine aniline blue staining procedure for suberin, lignin and callus in plant tissue. Protoplasma 146: 133–142.
Cavusoglu K, Kilic S and Kabar K, 2007. Some morphological and anatomical observations during alleviation of salinity (NaCl) stress on seed germination and seedling growth of barley by polyamines. Acta Physiology Plantarum 29: 551-557.
Cavusoglu K, Kilic S and Kabar K, 2008. Effects of some plant growth regulators on leaf anatomy of radish seedlings grown under saline conditions. Journal of Applied Biology Science 2: 47-50.
Fricke W, Bijanzadeh E, Emam Y and Knipfer T, 2014. Root hydraulics in salt-stressed wheat. Functional Plant Biology 41: 366-378.
Fricke W and Peters WS, 2002. The biophysics of leaf growth in salt-stressed barley: A study at the cell level. Plant Physiology 129: 374–388.
Hachez C, Moshelion M, Zelazny E, Cavez D and Chaumont F, 2006. Localization and quantification of plasma membrane aquaporin expression in maize primary roots: a clue to understand their role as cellular plumbers. Plant Molecular Biology 62: 305–323.
Hu Y, Camp KH and Schmidhalter U, 2000a. Kinetics and spatial distribution of leaf elongation of wheat (Triticum aestivum L.) under saline soil conditions. International Journal of Plant Science 161: 575–582.
Hu Y, Fromm J and Schmidhalter U, 2005. Effect of salinity on tissue architecture in expanding wheat leaves. Planta 220: 838-848.
Hu Y and Schmidhalter U, 2000. Reduced cellular cross-sectional area in the leaf elongation zone of wheat causes a decrease in dry weight deposition under saline conditions. Australian Journal of Plant Physiology 28: 165-170.
Hu Y, Schnyder H and Schmidhalter U, 2000b. Carbohydrate accumulation and partitioning in elongating leaves of wheat in response to saline soil conditions. Australian Journal of Plant Physiology 27: 363–370.
Hwang YH and Chen SC, 1995. Anatomical responses in Kandelia candel (L.) Druce seedlings growing in the presence of different concentrations of NaCl. Botanical Bulletin Acta Sinica 36: 181-188.
Kuo J, O’Brien TP and Canny MJ, 2004. Pit-field distribution, plasmodesmatal frequency and assimilate flux in the mestome sheath cells of wheat. Planta 121: 97–118.
Lazof DB, Bernstein N and Lauchli A, 1991. Growth and development of the Lactuca sativa shoot as affected by NaCl stress: consideration of leaf developmental stages. Botanical Gazette 152: 72-76.
Martre PJ, Cochard L and Durand H, 2000. Changes in axial hydraulic conductivity along elongating leaf blade sin relation to xylem maturation in tall fescue. New Phytology 146: 235–247.
Munns R, 2005. Genes and salt tolerance: bringing them together. New Phytology 167: 645-663.
Munns R, Richard A, Lauchli J and Lauchli A, 2006. Approaches to increasing the salt tolerance of wheat and other cereals. Journal of Experimental Botany 57: 1025–1043.
Munns R, Schachtman DP and Condon AG, 1995. The significance of a two phase growth response to salinity in wheat and barley. Australian Journal of Plant Physiology 22: 561–569.
Ola H, Elbar A, Reham E, Farag S, Eisa S and Habib SA, 2012. Morpho-anatomical changes in salt stressed kallar grass (Leptochloa fusca L. Kunth). Research Journal of Agriculture Biology Science 8: 158-166.
Smith LG and Hake S, 1992. The initiation and determination of leaves. Plant Cell 4: 1017-1027.
Taleisnik E, Rodriguez AA, Bustos D, Erdei L, Ortega L and Senn ME, 2009. Leaf expansion in grasses under salt stress. Journal of Plant Physiology 166: 1123-1140.
Tang P and Boyer AC, 2002. Growth induced water potentials and the growth of maize leaves. Journal of Experimental Botany 53: 489–503.
Trivett CL and Evert RF, 1998. Ontogeny of the vascular bundles and contiguous tissues in the barley leaf blade. International Journal of Plant Science 159: 716–723.
Vysotskaya L, Hedley PE, Sharipova G, Veselov D, Kudoyarova G, Morris J and Jones G, 2010. Effect of salinity on water relations of wild barley plants differing in salt tolerance. AOB Plants 24: 401-408.
Zadoks JC, Chang TT and Konzak, CF, 1974. A decimal code for the growth stages of cereals. Weed Research 14: 415-421.