Role of Exogenous Application of Auxin on Antioxidant Enzyme Activities in Rice Under Salt Stress

Document Type : Research Paper

Author

Department of Agronomy, College of Agriculture, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran

Abstract

Phytohormones such as auxin are known to be involved in alleviating the detrimental effects of salinity by modulating the activity of enzymatic antioxidants and improving antioxidant system, which help in sustaining plant growth. The present study envisaged revealing the role of exogenous application of indole-3-acetic acid (IAA) in improving defense mechanisms in two genotypes (FL485 and IR29, salt tolerant and salt susceptible, respectively) of rice against NaCl stress. The results showed that salt stress led to an increase in the ascorbic acid (AsA) content of the tolerant cultivar suggesting that oxidative defense system was very operative in this cultivar. Exogenous application of IAA further increased the activities of AsA and ascorbate oxidase in the salt stressed FL485, whereas such effect was not observed in the susceptible cultivar (IR29). IAA was found to be more effective in enhancing the activities of α-tocopherol in salt stressed rice plants with more efficiently in the salt tolerant cultivar. In addition, IAA markedly increased hydrogen peroxide (H2O2) concentration in both cultivars as compared to untreated plants. Lipid peroxidation levels of both cultivars under salt treatment showed no change with foliar applications of IAA.
 

Keywords

Article Title [Persian]

نقش کاربرد اکسین بر فعالیت‏های آنزیمی آنتی‏اکسیدانی در برنج تحت تنش شوری

Abstract [Persian]

مشخص شده است که فیتو هورمون­ها، از جمله اکسین در کاهش اثرات نامطلوب تنش شوری از طریق بهبود سیستم آنتی‏اکسیدانی و فعالیت آنزیمی به حفظ رشد گیاهان کمک می‏کنند. در این پژوهش نقش کاربرد هورمون اکسین در بهبود سازوکارهای دفاعی دو رقم برنج متحمل به شوری، FL485، و حساس به شوری، IR29، بررسی شد. تنش شوری موجب افزایش محتوای اسید آسکوربیک در رقم متحمل شد. این وضعیت نشان داد که سیستم دفاعی علیه تنش اکسیداتیو در این رقم بسیار فعال عمل می‏کند. کاربرد خارجی اکسین موجب افزایش فعالیت اسید آسکوربیک و آسکوربات اکسیداز در رقم متحمل به شوری شد، در حالی که چنین اثری در رقم حساس مشاهده نشد. اکسین در افزایش فعالیت آلفاتوکوفرول در گیاهان تنش دیده برنج به ویژه در رقم متحمل بسیار موثر عمل کرد. علاوه براین، اکسین موجب افزایش غلظت پراکسید هیدروژن در هر دو رقم در مقایسه با شرایط فاقد اکسین شد. با کاربرد اکسین روی برگ‏ها، غلظت لیپیدهای اکسید شده در هر دو رقم تحت شرایط تنش شوری تغییر نیافت.
 

Keywords [Persian]

  • آسکوربات اکسیداز (AAO)
  • اسید آسکوربیک (AsA)
  • اکسین (IAA)
  • برنج
  • شوری

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Journal of Plant Physiology and Breeding
Volume 6, Issue 2
December 2016
Pages 49-61

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