Increasing Salt Tolerance and Antioxidant Activity in Artemisia aucheri by H2O2-Priming

Document Type : Research Paper

Authors

Biology Department, Faculty of Sciences, Shahrekord University, Shahrekord, Iran

Abstract

Recent studies have introduced seed priming with H2O2 as an effective technique to alleviate abiotic stresses in plants. In the current study, accomplished at Faculty of Sciences, Shahrekord University, seeds of medicinal plant Artemisia aucheri were primed with H2O2 (0, 10, 50, 90 and 140 µM) and grown under salt stress (0 and 150 mM NaCl) for one month. Results showed a decrease in H2O2 and malonyldialdehyde concentrations by H2O2 priming leading to diminish lipid peroxidation at the cellular level. Moreover, seed priming with H2O2 (particularly at 90 µM) increased biomass, total water content, chlorophyll (a+b) and carotenoids concentrations, total phenolic content and antioxidant capacity in the plants from primed seeds under both normal and saline conditions. Higher activities of superoxide dismutase and catalase were observed in the primed A. aucheri with 90 µM H2O2, while the activity of ascorbate peroxidase was at the maximum level at 140 µM H2O2 priming condition. Additionally, hydroxyl and super oxide radicals scavenging activities were at the maximum level in the plants from primed seeds with 90 µM H2O2. Data revealed that H2O2 priming can induce salt tolerance in A. aucheri plants by adjusting physiological and metabolic processes such as photosynthesis, ROS scavenging and detoxification and brings about an improved growth and development in this species. Furthermore, H2O2 priming at 90 µM augmented antioxidant activity and reducing power in A. aucheri suggesting an increase in its medicinal properties.
 

Keywords


Article Title [فارسی]

افزایش فعالیت آنتی اکسیدانی و مقاومت به شوری در درمنه کوهی (Artemisia aucheri) باH2O2 –پرایمینگ

Abstract [فارسی]

 مطالعات اخیر، پرایمینگ بذر با H2O2 را به­عنوان روشی موثر برای تخفیف تنش­های غیرزیستی در گیاهان معرفی کرده­اند. در تحقیق حاضر که در دانشکده علوم داشگاه شهرکرد انجام گرفت، بذرهای گیاه دارویی درمنه کوهی(Artemisia aucheri)  با H2O2(صفر، 10، 50، 90 و 140 میکرومولار) پرایم شدند و به مدت یک ماه تحت تنش شوری ( 0 و 150 میلی مولار کلرید سدیم) کشت شدند. نتایج نشان گر کاهش غلظت H2O2 و مالون دی آلدهید در اثر این پرایمینگ بود که منجر به کاهش پراکسیداسیون لیپیدی در سطح سلول شد. علاوه بر این، پرایمینگ بذر با H2O2(به ویژه در 90 میکرومولار) باعث افزایش بیوماس، محتوای آب کل، غلظت کلروفیل کل و کاروتنوییدها، محتوای فنل کل و ظرفیت آنتی اکسیدانی در این گونه تحت هر دو شرایط عادی و تنش شوری گردید. بیشترین فعالیت سوپراکسید دیسموتاز، کاتالاز، فعالیت پاکروبی رادیکال­های هیدروکسیل و سوپراکسید در گیاهان درمنه کوهی که بذر آن­ها با 90 میکرومولار پراکسید هیدروژن پرایم شده بود، مشاهده شد. در حالی که فعالیت آسکوربات پراکسیداز در گیاهانی که بذر آن­ها با 140 میکرومولار پرایم شده بودند، به حداکثر رسید. نتایج نشان دادند که پرایمینگ با H2O2 می­تواند با تنظیم فرآیندهای فیزیولوژیک و متابولیسمی مانند فتوسنتز، پاکروبی انواع اکسیژن فعال و سمیت­زدایی باعث افزایش مقاومت به شوری در گیاهان درمنه کوهی و در نتیجه بهبود رشد و نمو در این گونه شود. علاوه براین، پرایمینگ با H2O2 (90 میکرومولار) فعالیت آنتی اکسیدانی و قدرت احیاکنندگی در درمنه کوهی را افزایش داد که می­تواند نشان­گر افزایش خواص دارویی این گیاه باشد.
 

Keywords [فارسی]

  • پیش تیمار با H2O2
  • درمنه کوهی
  • ظرفیت آنتی اکسیدانی
  • فعالیت پاکروبی رادیکال­ها
  • مقاومت به شوری
Aebi H, 1984. Catalase in vitro. Methods Enzymoogy 105: 121-126.
See comment in PubMed Commons belowApel K and Hirt H, 2004. Reactive oxygen species: metabolism, oxidative stress and signal transduction. Annual Review of Plant Biology 55: 373-399.
Arbona V, Flors V, Jacas J, García-Agustín P and Gómez-Cadenas A, 2003.  Enzymatic and Non-enzymatic antioxidant responses of Carrizo citrange, a salt-sensitive citrus rootstock, to different levels of salinity. Plant Cell Physiology 44 (4): 388–394.
Asghar G, Jalili M and Sadooghi E, 2012. Antimicrobial activity and chemical composition of essential oil from the seed of Artemisia aucheri Boiss. Jundishapur Journal of Natural Pharmaceutical Products (1): 11-15.
 Azevedo-Neto AD, Prisco JT, Enéas-Filho J, Medeiros JV and Gomes-Filho E, 2005. Hydrogen peroxide pre-treatment induces salt-stress acclimation in maize plants. Journal of Plant Physiology 162 (10): 1114-1122.
Azimian F and Roshandel P, 2015. Magnetic field effects on total phenolic content and antioxidant activity in Artemisia sieberi under salinity. Indian Journal of Plant Physiology 20 (3): 264-270.
Babaahmadi M, Amjad L and Roozbehani SH, 2013. The effect of allergenicity of Artemisia aucheri flowering taps in guinea pigs. International Journal of Agriculture and Crop Sciences 5 (18): 2079-2083.
Bahrami Karcondi M, Moshtaghyan SJ, Madani SH, Manzoni P, Adibi SH and Kazemi S, 2010. Effect of extract hydro-alcholi Artemisia aucheri on fibrosis induced pulmonary by bleomycin in rats. Journal of Shahrekord University Medical Science 3(4): 33-40 (In Persian with English abstract).
Bradford M, 1976. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein–dye binding. Annals of Biochemistry 72: 248-254.
Buchanan BB and Balmer Y, 2005. Redox regulation: a broadening horizon. Annual Review of PlantBiology 56: 187–220.
Chao YY and Kao CH, 2010. Heat shock-induced ascorbic acid accumulation in leaves increases cadmium tolerance of rice (Oryza sativa L.) seedlings. Plant and Soil 336: 39-48.
Chen DH, Ye HC and Li GF, 2000. Expression of a chimeric farnesyldiphosphate synthase gene in Artemisia annua L. transgenic plants via Agrobacterium tumefaciens-mediated transformation. Plant Science155: 179-185.
Dinani NJ, Asgary A, Madani H, Naderi G and Mahzoni P, 2010. Hypocholesterolemic and antiatherosclerotic effect of Artemisia aucheri in hypercholesterolemic rabbits. Pakistan Journal of Pharmaceutical Science 23 (3): 321-325.
Gao Y, Guo YK, Lin SH, Fang YY and Bai JG, 2010. Hydrogen peroxide pretreatment alters the activity of antioxidant enzymes and protects chloroplast ultrastructure in heat stressed cucumber leaves. Scientia Horticulturae 126: 20–26.
Gill SS and Tuteja N, 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48: 809-930.
Gondim FA, Gomes-Filho E, Costa JH, Mendes Alencar NL and Prisco JT, 2012. Catalase plays a key role in salt stress acclimation induced by hydrogen peroxide pretreatment in maize. Plant Physiology and Biochemistry 56: 62-71.
He L, Gao Zh and Li R, 2009. Pretreatment of seed with H2O2 enhances drought tolerance of wheat (Triticum aestivum L.) seedlings. African Journal of Biotechnology 8 (22): 6151-6157.
Hossain MA, Bhattacharjee S, Armin SM, Qian P, Xin W, Li H, Burritt DJ, Fujita M and Tran LP, 2015. Hydrogen peroxide priming modulates abiotic oxidative stress tolerance: insights from ROS detoxification and scavenging. Frontiers in Plant Science 6: 1-19.
Kato M and Shimizu S, 1985. Chlorophyll metabolism in higher plants. Plant Cell Physiology 26: 1291-1301.
Karuppanapandian T, Moon JC, Kim C, Manoharan K and Kim W, 2011. Reactive oxygen species in plants: their generation, signal transduction and scavenging mechanisms. Australian Journal of Crop Science 5 (6): 709-725.
Khajehzadeh MH, Zare-maivan H, Ghanati F and Sharifi M, 2014. Changes of enzymes activity and production of secondary metabolites of Artemisia aucheri in different altitudes and its relation to adaptation. Journal of Chemical Health Risks 4 (3): 57–66.
Krishnaiah D, Sarbatly R and Nithyanandam D, 2010. A review of the antioxidant potential of medicinal plant species. Food and Bioproducts Processing 24 (4-5): 506-515.
Ksouri R, Megdiche W, Debez A, Falleh H, Grignon C and Abdelly C, 2007. Salinity effects on polyphenol content and antioxidant activities in leaves of halophyte Cakile maritime. Plant Physiology and Biochemistry 45: 244-249.
Kukreja S, Nandwal AS, Sharma SK, Unvi V and Sharma  PK, 2005. Plant water status, H2O2 scavenging enzymes, ethylene evolution and membrane integrity of Cicer arietinum roots as affected by salinity. Biologia Plantarum 49 (2): 305-308.
Kumar M, Sirhindi G, Bhardwaj R, Kumar S and Jain G, 2010. Effect of exogenous H2O2 on antioxidant enzymes of Brassica juncea L. seedlings in relation to 24-epibrassinolide under chilling stress. Indian Journal of Biochemistry and Biophysics 47 (6): 378-382.
Kumaran A and Joel Karunakaran R, 2006. Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chemistry 97: 109-114.
Lin WC and Block GS, 2010. Can H2O2 application reduce chilling injury of horticultural crops? Acta Horticulture 875: 33-36.
Maisuthisakul P, Pasuk S and Ritthiruangdej P, 2008. Relationship of antioxidant properties and chemical composition of some Thai plants. Journal of Food Composition and Analysis 21: 229-240.
Mousaei Sanjerehei M, Jafari M, Mataji A, Baghestani Meybodi M and Bihamta MR, 2013. Influence of environmental factors on distribution of plant species in Nodushan rangelands of Yazd Province (Iran). Desert 18: 19-26 (In Persian with English abstract).
Nemat-Ala MM and Hassan NM, 2006. Changes of antioxidants levels in two maize lines following atrazine treatments. Plant Physiology and Biochemistry 44: 202–210.
Nag S, Saha K and Choudhuri MA, 2000. A rapid and sensitive assay method for measuring amine oxidase based on hydrogen peroxide-titanium complex formation. Plant Science 157: 157-163.
Ozgur R, Uzilday B, Sekmen AH and Turkan I, 2013. Reactive oxygen species regulation and antioxidant defence in halophytes. Functional Plant Biology 40: 832-847.
Pellicer J, Garnatje T and Valles J, 2011. Artemisia (Asteraceae): understanding its evolution using cytogenetic and molecular systematic tools, with emphasis on subgenus Dracunculus. Records in Advanced Pharmaceutical Sciences 2: 199-222.
Quan LJ, Zhang B, Shi WW and Li HY, 2008. Hydrogen peroxide in plants: a versatile molecule of the reactive oxygen species network. Journal of Integrative Plant Biology 50 (1): 2-18.
Roshandel P and Azimian F, 2015. Effects of magnetic field on growth and antioxidant capacity of Artemisia aucheri in normal or saline conditions. Biological Forum7 (2): 1095-1103.
Sairam RK, Veerabhadra Rao K and Srivastava GC, 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science 163: 1037-1046.
Shigeoka S, Ishikawa T, Tamoi M, Miyagawa Y, Takeda T, Yabuta Y and Yoshimura K, 2002. Regulation and function of ascorbate peroxidase isoenzymes. Journal of Experimental of Botany 53: 1205-1219.
Siahpoosh A and Amraee F, 2011. Antioxidant capacity of various extract of Asteragallus murinus Boiss. Journal of Shahid Sadoughi University of Medical Sciences 19 (4): 437-444 (In Persian with English abstract).
Strain HH and Svec WA, 1966. Extraction, separation, estimation and isolation of chlorophylls. In: Vernon LP and Seely GR (Eds). The Chlorophylls. Pp. 21–66. Academic Press, New York.
Tanou G, Filippou P, Belghazi M, Job D, Diamantidis G, Fotopoulos V and Molassiotis A, 2010. Oxidative and nitrosative-based signaling and associated post-translational modifications orchestrate the acclimation of citrus plants to salinity stress. Plant Journal 72: 585–599.
Tanou G, Job C, Rajjou L, Arc E, Belghazi M, Diamantidis G, Molassiotis A and Job D, 2009. Proteomics reveals the overlapping roles of hydrogen peroxide and nitric oxide in the acclimation of citrus plants to salinity. Plant Journal 60: 795–804.
Uchida A, Jagendorf AT, Hibino T, Takabe T and Takabe T, 2002. Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice. Plant Science 163: 515–523.
Wahid A, Perveen M, Gelani S and Basra SM, 2007. Pretreatment of seed with H2O2 improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins. Journal of Plant Physiology164 (3): 283-294.
Xu FJ, Jin CW, Liu WJ, Zhang YS and Lin XY, 2011. Pretreatment with H2O2 alleviates aluminum induced oxidative stress in wheat seedlings. Journal of Integrative Plant Biology 53: 44-53.
Yu CW, Murphy TM and Lin CH 2003. Hydrogen peroxide- induces chilling tolerance in mung beans mediated through ABA-independent glutathione accumulation. Functional Plant Biology 30: 955–963.