In Vitro Propagation of Damask Rose Using the Temporary Immersion System

Document Type : Research Paper


1 MSc, Department of Agricultural Biotechnology, Tarbiat Modares University, Tehran, Iran

2 Associate Prof., Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

3 Assistant Prof., Department of Agronomy and Plant Breeding, Faculty of Agriculture, Malayer University, Malayer, Iran


For centuries, Damask rose has been one of the most popular species of the rose family. Damask rose can be propagated using vegetative methods such as micropropagation. In this research, the use of temporary immersion system was reported for the first time for Damask rose micropropagation. For this reason, a traditional temporary immersion system was designed to facilitate its micropropagation. To optimize this system, the effects of the BAP concentration, immersion time, explant size, number of explants per temporary immersion unit were investigated in the independent experiments using completely randomized design. Temporary immersion system was also compared with the solid and liquid media. The highest shoot number per explant was obtained from a temporary immersion system using an immersion time of 3 min every 150 min (11.8 shoots), a modified MS medium with 6 mg l-1 BAP (12.33 shoots) and explants with shoot length of 4 cm (12.45 shoots). Results indicated that the temporary immersion system was significantly better than solid and liquid media for the shoot number per explant (10.66, 2.56 and 5.66, respectively).


Article Title [Persian]

ریزازدیادی گیاه گل محمدی با استفاده از سیستم غرقاب موقت

Abstract [Persian]

گیاه گل محمدی از قدیمی‌ترین گیاهان خانواده رز می‌باشد که استفاده از آن به گذشته‌ای دور باز می‌گردد. گیاه گل محمدی می‌تواند با روش‌های مختلفی از جمله ریزازدیادی تکثیر شود. در این تحقیق استفاده از سیستم غرقاب موقتی برای ریزازدیادی گل محمدی برای اولین بار گزارش شده است. برای این منظور سیستم غرقاب موقتی جهت ریزازدیادی این گیاه طراحی شد. به منظور بهینه‌سازی این سیستم، اثرات غلظت BAP، زمان غرقاب، ریزنمونه‌های باززایی شده با اندازه‌های مختلف، تعداد ریزنمونه در هر واحد سیتم غرقاب موقتی در آزمایش­های جداگانه و در قالب طرح کاملاً تصادفی مورد بررسی قرار گرفت. همچنین سیستم غرقاب موقت با محیط کشت جامد و مایع مقایسه شد. بر اساس نتایج حاصل بیشترین میانگین تعداد نوساقه در هر گیاهچه از سیستم غرقاب موقت با زمان غرقاب 3 دقیقه هر 150 دقیقه یکبار (8/11 نوساقه)، محیط کشت MS تغییر شکل یافته با 6 میلی­گرم در لیتر BAP (33/12 نوساقه) و ریزنمونه‌هایی با طول 4 سانتی­متر (45/12 نوساقه) به دست آمد. همچنین نتایج نشان داد که سیستم غرقاب موقتی به طور معنی‌داری بهتر از محیط کشت جامد و مایع از نظر میانگین تعداد نوساقه در هر گیاهچه بوده است (به ترتیب 66/10، 56/2 و 66/5).

Keywords [Persian]

  • ریزازدیادی
  • ریزنمونه
  • زمان غرقاب
  • گل محمدی
  • BAP
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