In Vitro Production of Bulblet in Galanthus transcaucasicus Fomin, an Endangered Medicinal Plant

Document Type : Research Paper


1 Department of Horticultural Science, Maragheh Branch, Islamic Azad University, Maragheh, Iran

2 Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

3 Department of Biology, Ahar Branch, Islamic Azad University, Ahar, Iran


To optimize conditions for micropropagating Galanthus transcaucasicus Fomin, the effect of explant type, different concentrations of sucrose, type and different concentrations of auxin were examined on bulblet production. For the induction of bulblets, three types of bulb scales were usesd as explants. A two-step sterilization procedure was applied. Assessments were made after incubation for 20 weeks at 23±1ºC with 16h photoperiods. Highest number of bulblets were proliferated culturing bulb scales on  modified MS medium supplemented with 2.0 mg.l-1 BA and 2.0 mg.l-1 IBA. About  27% of bulblets were bigger than 5 mm in diameter, suitable for acclimatization. With the increase of sucrose concentration, number of bulblets decreased, but their size increased. This protocol provided a basis for future study on large-scale multiplication system for commercial nurseries of Galanthus transcaucasicus.


Article Title [Persian]

تولید درون شیشه‌ای پیازچه‌های گیاه در حال انقراض Galanthus transcaucasicus Fomin

Abstract [Persian]

جنس گالانتوس متعلق به تیره آماریلیداسه بوده که پراکنش آن در اروپا، آسیای صغیر و منطقه قفقاز است. آلکالوئیدهای خاصی در گیاهان این تیره وجود دارد که به دلیل داشتن خواص دارویی توجه پژوهشگران را به خود جلب نموده است. در پژوهش حاضر اثر غلظت‌های مختلف ساکاروز و چند نوع اکسین بر روی تولید پیازچه درون شیشه‌ای مورد آزمایش قرار گرفت. پیازهای گالانتوس با روش دو مرحله‌ای ضدعفونی شدند. پیازها بعد از تیمار با اتانول 70 درصد به مدت یک دقیقه، به مدت 20 دقیقه در هیپوکلریت سدیم 5 درصد قرار گرفته و سپس پیازهای نصف شده به مدت 10 دقیقه در هیپوکلریت سدیم 5/2 درصد قرار داده شدند. بر اساس نتایج حاصل، بیشترین پیازچه در محیط کشت MS تغییر یافته حاوی 2 میلی‌گرم در لیتر بنزیل آمینوپورین (BAP) و 2 میلی‌گرم در لیتر ایندول بوتیریک اسید (IBA) به دست آمد که در این حالت 27 درصد پیازچه ­ها بزرگتر از 5 میلی‌متر بوده و مناسب سازش به شرایط برون‌شیشه‌ای بودند. همچنین با افزایش غلظت ساکاروز، تعداد پیازچه‌ها کمتر شد ولی به اندازه آن­ها افزوده شد. این تحقیق دستورالعملی را جهت تولید گونه خزری جنس گالانتوس در خزانه در مقیاس بزرگ فراهم می‌نماید.

Keywords [Persian]

  • ریزازدیادی
  • کشت بافت
  • گل برفی
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