Drought Stress in Iranian Endemic Savory (Satureja rechingeri): In vivo and In vitro Studies

Document Type : Research Paper


1 Department of Plant Breeding, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

2 Research Institute of Forests and Rangelands of Iran, Tehran, Iran


The importance of different species of savory is due to having phenolic compounds such as carvacrol and thymol in the essential oil and rosmarinic acid in the extract, having strong antioxidant and antimicrobial effects. This study was conducted on five ecotypes of Satureja rechingeri in in vitro and in vivo experiments. After determining the best shooting and rooting medium, micropropagation was done and clones were prepared. Water stress treatments were prepared by increasing agar up to 100% followed by selecting the most tolerant and sensitive clones. In the in vivo experiment, effect of water stress was studied in a greenhouse with irrigation withhold and sampling at five stages. Ten morphological and physiological characters were recorded from both experiments. The tolerant clone had superior tolerance to water deficit. Increasing the agar concentration up to 66% had no effects on rooting and the growth rates of shoots but more increase led to a sharp reduction in the growth rate and root differentiation. In the greenhouse, the tolerant clone was tolerated the stress up to nine days without showing any symptoms, but the continuation of stress led to a sharp increase in proline and soluble sugars and the reduction of plant pigments and leaf osmotic potential. This research was a kind of drought simulation at in vitro condition, performed for the first time for identification and screening of drought tolerant clones of savory.


Article Title [Persian]

اثر تنش خشکی در شرایط درون شیشه‌ای و گلخانه‌ای بر مرزه بومی ایرانی Satureja rechingeri

Abstract [Persian]

اهمیت گونه‌های مختلف مرزه به علت دارا بودن ترکیبات فنلی کارواکرول و تیمول در اسانس و اسیدهای فنلی نظیر اسید رزمارینیک در عصاره است که دارای اثرات آنتی اکسیدانی و آنتی میکروبی قوی می‌باشند. این تحقیق روی پنج اکوتیپ  Satureja rechingeri در دو مرحله آزمایشگاهی و گلخانه‌ای انجام گرفت. بعد از تعیین بهترین محیط شاخه‌زایی و ریشه‌زایی، ریزازدیادی انجام و کلون‏ها تهیه شدند. تیمارهای تنش کم آبی با افزایش میزان غلظت آگار تا 100 درصد تهیه و متحمل‌ترین و حساس‌ترین کلون انتخاب و به گلخانه منتقل گردیدند. در شرایط گلخانه‌ای نیز با قطع آبیاری و نمونه‌برداری در پنج مرحله، تنش خشکی مورد بررسی قرار گرفت. در این تحقیق 10 صفت مورفولوژیک و فیزیولوژیک در محیط درون شیشه‌ای و گلخانه‌ای اندازه­گیری شد. کلون متحمل مقاومت بالایی نسبت به تنش کم آبی داشت به طوری که افزایش غلظت آگار تا 66% هیچ تاثیری بر میزان ریشه‌زایی و رشد نوشاخه‌ها نداشت. در عین حال افزایش بیشتر آگار منجربه کاهش شدید رشد و ناتوانی در ریشه‌زایی شد. در مرحله گلخانه نیز کلون متحمل تا نه روز تنش را بدون نشان دادن علائم ظاهری تحمل نمود ولی ادامه تنش منجر به افزایش شدید پرولین و قندهای محلول و کاهش رنگیزه‌های گیاهی و پتانسیل اسمزی برگ گردید. این تحقیق نوعی شبیه‌سازی خشکی در شرایط آزمایشگاهی است که به منظور تولید و غربال کلون‌های مقاوم به خشکی برای اولین بار در گیاه مرزه اجرا شد.

Keywords [Persian]

  • آگار
  • تنش آبی
  • ریزازدیادی
  • صفات مورفولوژیک و فیزیولوژیک
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