Response of Two Indica Rice Varieties to Salt Stress

Document Type : Research Paper


Department of Agronomy, College of Agriculture, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran.


Salinity is one of the most challenging problems that adversely affects growth and development of plants. Therefore, understanding of the mechanisms that enable plants to adapt to salinity stress will ultimately help in the selection of stress tolerant cultivars for exploiting saline soils. The main objective of this study was to examine the effects of NaCl on some physiological and biochemical characteristics of two rice varieties, IR29 (salt sensitive) and FL485 (salt tolerant), exhibiting different sensitivities to NaCl. NaCl induced a progressive increment in Na+ concentration of both cultivars, however, it was more marked in the sensitive cultivar IR29. A higher level of sugar and a delay in chlorophyll degradation together with less chlorophyll degradation were observed in the salt tolerant rice. Salt stress may promote sugar accumulation, thus preventing the degradation of chlorophyll. Salinity stress induced an accumulation of starch in cv. FL485. It is possible that adjusted carbon partitioning could have an important implication on salinity tolerance. It is suggested that allocation of sugars into starch may involve in salinity tolerance by avoiding metabolic alteration.


Article Title [Persian]

تجمع قند، جذب سدیم و پتاسیم و محتوای کلروفیل دو رقم برنج در عکس العمل به شوری

Abstract [Persian]

شوری یکی از مشکلات چالش برانگیزی است که تاثیر نامطلوبی بر رشد و توسعه گیاهان دارد. از این رو، درک مکانیسم‏هایی که گیاهان را قادر به انطباق با تنش شوری می‏کند در انتخاب ارقام متحمل جهت بهره برداری از خاک­های شور کمک خواهد کرد. هدف اصلی از این مطالعه بررسی اثرات شوری (NaCl)  بر برخی از خصوصیات فیزیولوژیکی و بیوشیمیایی دو رقم برنج حساس (IR29) و متحمل (FL485) بود. شوری موجب افزایش تدریجی غلظت سدیم در هر دو رقم حساس و متحمل شد. البته این افزایش در رقم حساس  (IR29) بیشتر بود. رقم متحمل به شوری غلظت بالایی از قندهای محلول و تجزیه دیرتر کلروفیل را نشان داد. علاوه براین، تجزیه کلروفیل نیز در رقم متحمل کمتر بود. احتمالا تحریک تجمع قند ناشی از تنش شوری مانع از تجزیه کلروفیل شده است. شوری همچنین موجب تجمع نشاسته در رقم FL485 شد. ممکن است که تنظیم تسهیم کربن فتوسنتزی در ایجاد تحمل به شوری نقش داشته باشد. از این رو به نظر می‏رسد که اختصاص قندها در مسیر ساخت نشاسته بجای قرار گرفتن در سایر مسیرهای متابولیکی در ایجاد تحمل به شوری نقش دارد.

Keywords [Persian]

  • برنج
  • تجزیه کلروفیل
  • شوری
  • نشاسته
Ashraf M, and Fatima H. 1995. Responses of some salt tolerant and salt sensitive lines of safflower (Carthamus tinctorius L.). Acta Physiol Plant 17: 61–71.
Azadi A, Hervan EM, Mohammadi SA, Moradi F, Nakhoda B, Vahabzade M, andMardi M, 2011. Screening of recombinant inbred lines for salinity tolerance in bread wheat (Triticum aestivum L.). African Journal of Biotechnology 10: 12875–12881.
Bagheri A, and Sadeghipour O, 2009. Effects of salt stress on yield, yield components and carbohydrates content in four hullless barley (Hordeum vulgare L.) cultivars. Journal of Biological Science 9(8): 909-912.
Balibrea ME, Amico JD, Bolarin MC, and Perez-Alfocea F, 2000. Carbon partitioning and sucrose metabolism  in tomato plants growing under salinity. Physiology Plant 110: 503-512.
Basu PS, and Minhas JS, 1991. Heat tolerance and assimilate transport in different potato genotype. Journal of Experimental of Botany 42: 861-866.
Cram WJ, 1976. Negative feedback regulation of transport in cells. The maintenance of turgor, volume and nutrient supply. Encyclopedia Plant Physiology 2: 284-316.
Dasgan HY, Koc S. 2009. Evaluation of salt tolerance in common bean genotypes by ion regulation and searching for screening parameters Journal of Food Agriculture and Environment 7: 363–372.
Dkhil BB, and Denden M, 2010. Salt stress induced changes in germination, sugars, starch and enzyme of carbohydrate metabolism in Abelmoschus esculentus (L.) moench seeds. African Journal of Agriculture Research 5(6), 408-415.
Dubey RS, and Singh AK, 1999. Salinity induces accumulation of soluble sugars and alters the activity of sugar metabolizing enzymes in rice plants. Biological plant 42: 233-241.
Fang Z, Bouwkamp J, and Solomos T, 1998. Chlorophyllase activities and chlorophyll degradation during leaf senescence in nonyellowing mutant and wild type of Phaseolus vulgaris L. Journal of  Experimental Botany 49: 503 – 510.
Gadallah MAA, 1999. Effect of kinetin on growth, grain yield and some mineral elements in wheat plants growing under excess salinity and oxygen deficiency. Plant Growth Regulation 27: 63–74.
Garg AK, Kim JK, Owens TG, Ranwala AP, Do Choi Y, Kochain LV, and Wu RJ, 2002. Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses. Proceeding National of Academic Science USA 99: 15898-15903.
Gibson SI, 2000.Plant sugar-response pathways: part of a complex regulatory web. Plant Physiology 124: 1532–1539.
Heidari M, 2012. Effects of salinity stress on growth, chlorophyll content and osmotic components of two basil (Ocimum basilicum L.) genotypes. African Journal of Biotechnology 11: 379–384.
Ismail AM, Heuer S, Thomson MJ, and Wissuwa M, 2007. Genetic and genomic approaches to develop rice germplasm for problem soils. Plant Molecular Biology 65: 547-570.
Khelil A, Menu T, and Ricard B, 2007. Adaptive response to salt involving carbohydrate metabolism in leaves of a salt-sensitive tomato cultivars. Plant Physiology and Biochemistry 45: 551–559. 
Krapp A, and Stitt M, 1995. An evaluation of direct and in direct mechanisms for the sink-regulation of photosynthesis in spinach: changes in gas exchange, carbohydrates, metabolites, enzymes activities and steady-state transcript levels after cold-girdling source leaves. Planata 186: 58-65.
Kronzucker HJ, Szcerba MW, Schulze LM, and Britto DT, 2008. Non-reciprocal interactions between K and Na ions in barley (Hordeum vugare L.). Journal of Experimental Botany 59: 1-9.
Loggini B, Scartazza A, Brugnoli E, Navari-Izzo F, 1999. Antioxidant defense system, pigment composition, and photosynthetic efficiency in two wheat cultivars subjected to drought. Plant Physiology 119: 1091–1099.
Lorenzen JH, Ewing E, 1992. Starch accumulation in leaves of potato (Solanum tuberosum L.) during the first 18 days of photoperiod treatment. Annul Botany69: 481-485.
Marcar NE, 1987. Salt tolerance in the genus Lolium (ryegrass) during germination and growth. Australian Journal of Agricultural Research. 38: 297–307.
Moradi F, Ismail AM, Gregorio GB, Egdane JA, 2003. Salinity tolerance of rice during reproductive development and association with tolerance at the seedling stage. Indian Journal of Plant Physiology 8: 276–287.
Morsy MR, Jouve L, Hausman JF, Hoffmann L, Stewart JM, 2007. Alteration of oxidative and carbohydrate metabolism under abiotic stress in two rice (Oryza sativa L.) genotypes contrasting in chilling tolerance. Journal of Plant Physiology 164: 157–167.
Munns R, and Tester M, 2008. Mechanisms of salinity tolerance.Annual Review of Plant Physiology 59: 651-681.
Munns R, Hare RA, James RA, and Rebetzke GJ, 2000. Genetic variation for improving the salt tolerance of durum wheat. Australian Journal of Agriculture Research 51: 69–74.
Munns R, and James RA, 2003. Screening methods for salinity tolerance: a case study with tetraploid wheat. Plant Soil 253: 201–218.
Naureen G, and Naqvi FN, 2010. Salt tolerance classification in wheat genotypes using reducing sugar accumulation and growth characteristic. Emir Journal Food Agriculture 22(4): 308-317.
Navarro JM, Martinez V, and Carvajal M, 2000. Ammonium, bicarbonate and calcium effects on tomato plants gown under saline conditions. Plant Science 157: 89–96.
Nemati I, Moradi F, Gholizadeh S, Esmaeili MA, and Bihamta MR, 2011. The effect of salinity stress on ions and soluble sugars distribution in leaves, leaf sheaths and roots of rice (Oryza sativa L.) seedlings. Plant Soil and Environment 57: 26–33.
Praxedes SC, De Lacerda CF, Ferreira TM, Prisco JT, DaMatta FM, and Gomes- Filho E, 2011. Salt tolerance is unrelated to carbohydrate metabolism in cowpea cultivars. Acta Physiology of Plant 33: 887–896.
Price J, Laxmi A, Martin SK, and Kang JC, 2004. Global transcription profiling reveals multiple sugar signal transduction mechanisms in Arabidopsis. Plant Cell 16:2128–2150.
Rosa M, Hilal M, Gonza´ lez JA, and Prado FE, 2004. Changes in soluble carbohydrates and related enzymes induced by low temperature during early developmental stages of quinoa (Chenopodium quinoa) seedlings. Journal of  Plant Physiology 161:683–689.
Saeedipour S, 2014. The Combined Effects of Salinity and Foliar Spray of Different Hormones on Some Biological Aspects, Dry Matter Accumulation and Yield in Two Varieties of Indica Rice Differing in Their Level of Salt Tolerance. Proceeding National Academic of  Science India B 84(3): 721–733.
Santos HP, and Buckeridge MS, 2004. The role of the storage carbon of cotyledons in the establishment of seedlings of Hymenaea courbaril under different light conditions. Annual Botany 94: 819–830.
Smeekens S, 2000. Sugar-induced signal transduction in plants. Annual Review of Plant Physiology and Plant Molecular Biology 51: 49–81.
Thakur M, and Sharma AD, 2005. Salt stress and phytohormone (ABA)- induced changes in germination, sugar and enzymes of carbohydrate metabolism in sorghum bicolor (L.) moenvh seeds. Journal of Agriculture and Social Sciences 18: 1813-2235.
Thomson MJ, de Ocampo M, Egdane J, Rahman MR, Sajise A G, Adorada DL, Tumimbang-Raiz E, Blumwald E, Seraj Z I, and Singh RK, 2010. Characterizing the Saltolquantitative trait locus for salinity tolerance in rice. Rice 3: 148-160.
Tirol-Padre A, and Ladha JK, 2004. Assessing the reliability of permanganate-oxidizable carbon as an index of soil labile carbon. Soil Science Society of American Journal 68: 969–978.
Yancey PH, Clark ME, Hand SC, Bowlus RD, and Somero GN, 1982. Living with water stress: evolution of osmolyte systems. Science 217:1214-1222.
Zhang ZH, Qiang L, Song HX, Rong XM, and Ismail AM, 2012. Responses of different rice (Oryza sativa L.) genotypes to salt stress and relation to carbohydrate metabolism and chlorophyll content. African Journal of Agriculture Research 7: 19–27.