Genetic Variability and QTL Mapping for Seed Germination Characters under Water Stress in Sunflower



The objective of this research was to study the genetic control of germination variables under normal and water deficit conditions in sunflower. A population of 100 recombinant inbred lines obtained from a cross between PAC2 and RHA266 was used in the present study. The experiment was performed as a randomized complete block design with three replications. Germination variables were time to starting germination (TSG), time to maximum germination (TMG), time to 50% germination (T50%G) and percentage of seed germination (PSG). The putative causes of variation in germination, oil content (OC) and 1000 grain weight (1000GW) were also measured. Positive correlations were obtained between different times to germination for normal and stress conditions. Positive correlations were observed between PSG and OC, as well as between 1000 GW and the different times to germination. Several non-specific QTLs to water deficit were detected for germination parameters. “HA1837”, “SSL27“and “ORS671_1” were the SSR markers associated with QTLs detected for germination characters independent of germination environments. The most important specific QTL was associated with “ORS677” SSR marker in the water stress condition, explaining 24% of the phenotypic variance for PSG.    


Article Title [Persian]

تنوع ژنتیکی و مکان‌یابی QTL برای صفات جوانه زنی در آفتابگردان تحت شرایط تنش کم‌آبی

Abstract [Persian]

این تحقیق روی کنترل ژنتیکی صفات جوانه زنی در شرایط نرمال و تنش کم­آبی در آفتابگردان انجام گرفت. جمعیتی شامل 100 لاین اینبرد حاصل از تلاقی RHA266 ×PAC2  مورد استفاده واقع شد. آزمایش به صورت طرح بلوک کاملا تصادفی در سه تکرار در هر یک از شرایط انجام شد. متغیر­های مورد بررسی شامل زمان­های تا جوانه زنی و درصد جوانه زنی بودند. میزان روغن بذر و وزن 1000 دانه نیز اندازه گیری شد. همبستگی مثبت بین تمامی زمان­های تا جوانه زنی دیده شد. همچنین همبستگی مثبت بین درصد جوانه زنی و میزان روغن بذر و وزن 1000 دانه وجود داشت. تعدادی QTL غیراختصاصی برای شرایط محیطی مختلف در مورد صفات جوانه زنی به دست آمدند. نشانگر­های “ORS671_1”، “HA1837” و “SSL27“ برای صفات جوانه زنی، که مستقل از شرایط محیطی بودند، شناسایی شدند. QTL مهمی نیز برای درصد جوانه زنی در شرایط تنش کم­آبی که با نشانگر “ORS 677” پیوسته بوده و 24% از تغییرات این صفت را کنترل می­کرد، شناسایی شد.

Keywords [Persian]

  • تنش خشکی
  • زمان­های تا جوانه زنی
  • نشانگر
  • QTL
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