Cold-induced Changes of Proline, Malondialdehyde and Chlorophyll in Spring Canola Cultivars



Low temperature (LT) is an important environmental factor that limits the survival, productivity and geographical distribution of plants. Oil seeds are the second global food resources among which Brassica napus L. is the third annual oil seed in the world. In cold stress, some biochemical and physiological reactions occur in response to reactive oxygen species (ROS). Hence, the quantitative changes of proline, malondialdehyde (MDA) and chlorophyll (a, b, total) content were assessed in two spring canola (cv. Zarfam, cold tolerant and cv. Option 500, cold sensitive) seedlings exposed to early spring cold stress. They were first grown in a controlled growth room at 22/16 °C (day/night) and then at the 4th fully expanded leafy stage seedlings weretransferred to a cold environment (10/3 °C) for 7 d, or they were maintained continuously at 22/16 °C (Control). Leaf samples were harvested at days 0 (transferred day), 2, 4 and 7 of cold exposure period. Analysis of variance showed significant differences between the temperature treatments and also cultivars for all physiological traits. The cold tolerant cultivar showed remarkable less LT-induced decrease in chlorophylls (a, b, total) and increase in MDA and accumulation of proline compared to the cold sensitive cultivar. This assay verified the superior response of cold tolerant Zarfam canola to cold temperature.


Article Title [Persian]

تغییرات سرما-القایی پرولین، مالون دی‌آلدهید و کلروفیل در ارقام بهاره کلزا

Abstract [Persian]

دمای پایین یکی از فاکتورهای محیطی مهمی است که بقا، تولید و توزیع جغرافیایی گیاهان را محدود می‌کند. دانه‌های روغنی دومین منابع غذایی جهان هستند که از میان آن‌ها کلزا(Brassica napus L.)  سومین دانه روغنی یکساله در جهان می‌باشد. در تنش سرما، برخی واکنش‌های بیوشیمایی و فیزیولوژیکی در پاسخ به گونه‌های اکسیژن فعال اتفاق می‌افتد. از این رو، تغییرات کمی مقدار پرولین، مالون دی‌آلدهید و کلروفیل (کل، a، (b در گیاهچه‌های دو رقم کلزای بهاره (زرفام، رقم متحمل و Option 500، رقم حساس به سرما) که در معرض سرمای اولیه بهار قرار گرفتند، ارزیابی شدند. بذور ابتدا در یک اتاق رشد کنترل شده در دمای °C 16/22 (شب/ روز) رشد داده‌شدند و سپس در مرحله چهار برگی کامل، نیمی از گیاهچه‌ها در شرایط سرما (°C 3/10) (شب/ روز) برای هفت روز و نیمی دیگر در همان شرایط°C  16/22 (شب/ روز) باقی ماندند (شاهد). نمونه‌های برگی در روز‌های صفر (روز انتقال)، دوم، چهارم و هفتم دوره‌ی انتقال به سرما برداشت شدند. تجزیه واریانس تفاوت معنی‌داری در تیمار دما و رقم در همه صفات فیزیولوژیکی نشان داد. رقم متحمل در مقایسه با رقم حساس به سرما کاهش سرما-القایی کمتری در مقدار کلروفیل‌ها (a، b، کل) و افزایش در مقدار مالون دی‌آلدهید و انباشت مقدار پرولین در پاسخ به سرما نشان داد. این بررسی پاسخ برتر رقم متحمل زرفام را به تنش سرما تأیید کرد

Keywords [Persian]

  • پرولین
  • تنش سرما
  • کلروفیل
  • کلزا
  • لیپید پراکسیداسیون
  • Brassica napus
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