Antioxidant Isoenzymes Activities in Seedling Roots of Wheat Exposed to Drought Stress

Document Type : Research Paper

Abstract

Abstract
This study was conducted to evaluate the role of oxidative stress in roots of eight wheat genotypes with different drought tolerance, subjected to drought stress. For analyzing the changes of antioxidant enzymes, native PAGE analyses of protein extract were performed. The roots of wheat seedlings showed one unambiguous isoform of superoxide dismutase (SOD) and catalase (CAT). Eight isoforms of peroxidase (POX) were identified in the roots of wheat. The activities of SOD and POX isoforms decreased and the total activities of POX and CAT remained unchanged under the stress condition. The response of enzyme isoforms to drought were not the same for all isoforms of the antioxidant enzymes in the wheat genotypes, as POX isoforms showed the significant changes in the different drought tolerant genotypes. Significant interaction was observed between wheat group and stress treatments for total POX activity. POX total activity in the sensitive group of wheat genotypes was significantly higher than the tolerant group in the stress condition.
 

Keywords


Article Title [فارسی]

فعالیت ایزوآنزیم‌های آنتی اکسیدان در ریشه گیاهچه‌های گندم تحت تنش خشکی

Abstract [فارسی]

این مطالعه برای ارزیابی نقش تنش اکسیداتیودر ریشه گیاهچه­های هشت ژنوتیپ گندم پائیزه با درجه تحمل خشکی متفاوت تحت تنش خشکی (اسمزی) انجام شد.  برای ارزیابی تغییرفعالیت آنزیم­های آنتی­اکسیدان از سیستم الکتروفورز ناپیوسته در ژل اکریلامید افقی استفاده شد. در ریشه­­ گیاهچه­های گندم برای آنزیم کاتالاز (CAT) و سوپر اکسیددیسموتاز (SOD) یک ایزوفرم و برای پراکسیداز (POX) هفت ایزوفرم مشاهده شد. تحت شرایط تنش، فعالیت ­SOD و ایزوفرم­های POX کاهش یافت و فعالیت POX کل و CAT بدون تغییر باقی ماند. پاسخ تمامی ایزوفرم­های آنزیم­های آنتی­اکسیدان به خشکی در ژنوتیپ­های گندم یکسان نبود؛ به طوری که ایزوفرم­های POX تغییر معنی­داری در ژنوتیپ­های با درجه تحمل خشکی متفاوت نشان دادند. اثر متقابل معنی­داری بین گروه­های ژنوتیپ­های گندم و شرایط تنش برای فعالیت POX کل مشاهده شد. در شرایط تنش، فعالیت POX کل در گروه ژنوتیپ­های حساس گندم به طور معنی­داری بیشتر از گروه متحمل بود.
 

Keywords [فارسی]

  • یزوزیم
  • پراکسیداز
  • تنش خشکی
  • سوپر اکسیددیسموتاز
  • کاتالاز
  • گندم
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