Cold-induced Changes of Antioxidant Enzymes Activity and Lipid Peroxidation in Two Canola (Brassica napus L.) Cultivars

Authors

1 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

2 Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

Abstract

This study was conducted on two canola (Brassica napus) cultivars, Okapi a winter type and cold tolerant and Rgs003 a spring type and cold sensitive. Seedlings were grown in an environmentally controlled growth room with 16 h d-1 photoperiod at 22/16 °C (day/night, control). At the 4-leaf stage, half of pots were transferred to a cold growth room for 7 d at 10/3°C (day/night, cold treatment) and their leaves were harvested as required on 0, 2, 4 and 7 d as four sampling times. Superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) activity and the amount of malondialdehyde (MDA) in samples were assessed. Analysis of variance showed that winter canola had more SOD, CAT and APX activity, lower amount of either MDA or POD activities compared with the spring canola. This study indicated that antioxidant mechanisms caused more cold tolerance in the winter cultivar of canola compared with the spring cultivar.

Keywords

Article Title [Persian]

تغییرات سرما - القایی فعالیت آنزیم‌های آنتی‌اکسیدان و پراکسید شدن لیپید در دو رقم کلزا (Brassica napus L.)

Abstract [Persian]

این پژوهش روی دو رقم کلزا (Brassica napus)، اکاپی یک رقم زمستانه متحمل به سرما و Rgs003 یک رقم بهاره حساس به سرما، انجام گردید. گیاهچه‌ها در یک اتاق رشد کنترل شده محیطی با فتوپریود 16 ساعت در روز در دمای °C 16/22 (شب/روز، شاهد) رشد یافتند. در مرحله چهار برگی، نیمی از گلدان‌ها به اتاق رشد با دمای °C 3/10 (شب/روز، تیمار سرمایی) به مدت 7 روز منتقل شدند و به مقدار مورد نیاز از برگ‌ها در روزهای 0، 2، 4 و 7 به عنوان چهار زمان نمونه‌برداری برداشت شد. فعالیت آنزیم‌های سوپراکسید دیسموتاز (SOD)، پراکسیداز (POD)، کاتالاز (CAT) و آسکوربات پراکسیداز (APX) و مقدار مالوندآلدهید (MDA) در نمونه‌ها ارزیابی شد. نتایج تجزیه واریانس و مقایسه میانگین­ها، فعالیت بیشتر SOD، CAT و APX و فعالیت کمتر MDA و POD را در کلزای زمستانه در مقایسه با کلزای بهاره نشان داد. این پژوهش خاطر نشان ساخت که سازوکارهای آنتی‌اکسیدانی باعث مقاومت بیشتر سرما در رقم زمستانه کلزا در مقایسه با رقم بهاره گردید.
 

 

Keywords [Persian]

  • آنزیم‌های آنتی‌اکسیدان (APX
  • Cat
  • POD
  • SOD)؛ پراکسید شدن لیپید؛ تنش سرما؛ کلزا

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