Factor analysis of genetic diversity in some Iranian purslane accessions using agro-morphological traits

Articles in Press

Document Type : Research Paper

Authors

1 Department of Horticultural Science, Faculty of Agricultural Science and Natural Resources, University of Mohaghegh Ardabili, 7218759612, Ardabil, Iran.

2 Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, 5518779842, Maragheh, Iran.

Abstract

Objective: As a highly nutritious vegetable crop, purslane (Portulaca oleracea L.) is recognized for its dual role as both a leafy vegetable and a medicinal herb. This study aimed to assess the genetic diversity of 20 Iranian purslane accessions using selected agro-morphological traits.
Methods: Seeds from local purslane accessions were collected from different regions of Iran. The experiment was conducted under controlled greenhouse conditions, using a randomized complete block design with four replications. A total of 16 agro-morphological traits were measured. Phenotypic coefficient of variation was calculated for all traits to verify the existence of variability among the collected accessions. To group the accessions, factor analysis was performed to uncover a pattern of variability among the studied accessions. Only factors with eigenvalues greater than unity were retained and used.
Results: The results revealed considerable genetic variation among the genotypes, with the phenotypic coefficient of variation ranging from 11.66% (main stem length) to 51.00% (number of capsules per branch). High variability in traits such as total soluble solids and dry/fresh weight ratio, and relatively high variability for number of flowers per plant, dry shoot weight, number of lateral branches, number of branches per main stem, leaf width, leaf area, and leaf length, was observed, highlighting their potential value for future breeding efforts. Factor analysis identified five main factors accounting for 90.1% of the observed variability, with the first factor describing 48.6% of the variability and associated with plant biomass potential. The next factors emphasized the number of flowers and branches, leaf properties, the number of lateral branches, and total soluble solids, respectively. The accessions were categorized into three distinct categories, and Group A contained accessions with better biomass and some yield-related traits.
Conclusion: This research provided an understanding of genetic diversity in purslane, offering information for selecting favorable genotypes for breeding programs aimed at enhancing biomass and yield performance. Accessions from Group A, such as those from Bushehr, Isfahan, and Maragheh areas, Iran, were recommended for further evaluation and potential cultivar release for rainfed, semiarid conditions.

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References

Alam A, Juraimi AS, Yusop MR, Hamid AA, Hakim A. 2014. Morpho-physiological and mineral nutrient characterization of 45 collected purslane (Portulaca oleracea L.) accessions. Bragantia 73(4): 426-437. https://doi.org/10.1590/1678-4499.253
Amirul Alam M, Juraimi AS, Rafii MY, Hamid AA, Kamal Uddin M, Alam MZ, Latif MA. 2014. Genetic improvement of purslane (Portulaca oleracea L.) and its future prospects. Mol Biol Rep. 41: 7395-7411. https://doi.org/10.1007/s11033-014-3628-1
Borsai O, Al Hassan M, Boscaiu M, Sestras RE, Vicente O. 2018. The genus Portulaca as a suitable model to study the mechanisms of plant tolerance to drought and salinity. EuroBiotech J. 2(2): 104-113. https://doi.org/10.2478/ebtj-2018-0014
Carrascosa A, Pascual JA, Ros M, Petropoulos SA, Alguacil MDM. 2023. Agronomical practices and management for commercial cultivation of Portulaca oleracea as a crop: a review. Plants. 12(6): 1246. https://doi.org/10.3390/plants12061246
Coulibaly M, Bodjrenou G, Fassinou Hotègni NV, Akohoue F, Agossou CA, Azon CF, Matro X, Bello S, Adjé COA, Sanou J, et al. 2024. Genotype by environment interaction and stability analysis of three agronomic traits in Kersting's groundnut (Macrotyloma geocarpum) using factor analytic modeling and environmental covariates. Crop Sci. 64(4): 2095-2115. https://doi.org/10.1002/csc2.21249
Egea-Gilabert C, Ruiz-Hernández MV, Parra MÁ, Fernández JA. 2014. Characterization of purslane (Portulaca oleracea L.) accessions: suitability as ready-to-eat product. Sci Hortic. 172: 73-81. https://doi.org/10.1016/j.scienta.2014.03.051
El-Bakatoushi R, Alframawy AM, Samer M, El-Sadek L, Botros W. 2013. Evolution of the Portulaca oleracea L. aggregate in Egypt on molecular and phenotypic levels revealed by morphology, inter-simple sequence repeat (ISSR) and 18S rDNA gene sequence markers. Flora: Morphol Distrib Funct Ecol Plants. 208(7): 464-477. https://doi.org/10.1016/j.flora.2013.07.008
Fathi S, Kharazmi M, Najafian S. 2019. Effects of salicylic acid foliar application on morpho-physiological traits of purslane (Portulaca olaracea L.) under salinity stress conditions. J Plant Physiol Breed. 9(2): 1-9. https://doi.org/10.22034/jppb.2019.10439
He J, Gan JH, S, Qin L. 2023. Productivity, photosynthetic light-use efficiency, nitrogen metabolism and nutritional quality of C4 halophyte Portulaca oleracea L. grown indoors under different light intensities and durations. Front Plant Sci. 14: 1106394. https://doi.org/10.3389/fpls.2023.1106394
Kumar A, Sreedharan S, Kashyap AK, Singh P, Ramchiary N. 2022. A review on bioactive phytochemicals and ethnopharmacological potential of purslane (Portulaca oleracea L.). Heliyon 8(1): 1e08669. https://doi.org/10.1016/j.heliyon.2021.e08669
Mohebodini M, Khalili-Baseri I, Farmanpour-Kalalagh K, Sabaghnia N. 2024. Morphological characterization of some collected native purslane (Portulaca oleracea L.) genotypes. Contrib Bot. 59: 7-20. https://doi.org/10.24193/Contrib.Bot.59.1
Mulry KR, Hanson BA, Dudle DA. 2015. Alternative strategies in response to saline stress in two varieties of Portulaca oleracea (purslane). PloS One 10(9): e0138723. https://doi.org/10.1371/journal.pone.0138723
Naeem MY, Jabran K, Özden M, Bakhsh A. 2022. Assessment of morphological and biochemical characteristics of common purslane (Portulaca oleracea L.) accessions. Pak J Agric Sci. 59(6): 1017-1028. https://doi.org/10.21162/PAKJAS/22.101
Petropoulos SA, Fernandes Â, Dias MI, Vasilakoglou IB, Petrotos K, Barros L, Ferreira ICFR. 2019. Nutritional value, chemical composition and cytotoxic properties of common purslane (Portulaca oleracea L.) in relation to harvesting stage and plant part. Antioxidants. 8(8): 293. https://doi.org/10.3390/antiox8080293
Sabaghnia N, Janmohammdi M. 2025. Genetic diversity in rye (Secale cereale L.) landraces based on agro-morphological traits under the semi-arid conditions of Iran. Genet Res Crop Evol. 72(5): 5127-5138. https://doi.org/10.1007/s10722-024-02188-9
Sabaghnia N, Ahadnezhad A, Janmohammadi M. 2024. Genetic diversity in safflower (Carthamus tinctorius L.) germplasm for vegetative and agronomic characteristics in semi-arid regions. Plant Genet Res. 22(4): 241-250. https://doi.org/10.1017/S1479262124000200
Salgotra RK, Chauhan BS. 2023. Genetic diversity, conservation, and utilization of plant genetic resources. Genes. 14(1): 174. https://doi.org/10.3390/genes14010174
Sdouga D, Branca F, Kabtni S, Di Bella MC, Trifi-Farah N, Marghali S. 2020. Morphological traits and phenolic compounds in Tunisian wild populations and cultivated varieties of Portulaca oleracea L. Agronomy. 10: 948. https://doi.org/10.3390/agronomy10070948
Singh RK, Prasad A, Muthamilarasan M, Parida SK, Prasad M. 2020. Breeding and biotechnological interventions for trait improvement: status and prospects. Planta. 252: 54. https://doi.org/10.1007/s00425-020-03465-4
Srivastava R, Srivastava V, Singh A. 2023. Multipurpose benefits of an underexplored species purslane (Portulaca oleracea L.): a critical review. Environ Manage. 72(2): 309-320. https://doi.org/10.1007/s00267-021-01456-z
Yang X, Li J, Zhao T, Mo L, Zhang J, Ren H, Gao Y. 2019. Variation and heritability of morphological and physiological traits among Leymus chinensis genotypes under different environmental conditions. J Arid Land. 11: 66-74. https://doi.org/10.1007/s40333-018-0018-x
 
Journal of Plant Physiology and Breeding

Articles in Press, Accepted Manuscript
Available Online from 12 December 2025

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