Influence of Arbuscular mycorrhiza fungi and Bradyrhizobium japonicum on the fatty acids profile of soybean (Glycine max L.) under drought stress conditions

Document Type : Research Paper

Authors

1 Department of Agronomy, Gonbad Kavous University, Gonbad Kavous, Iran

2 Department of Agricultural Sciences, Payame Noor University, Tehran, Iran

Abstract

Objective: Water deficiency changes the composition of soybean fatty acids. Arbuscular mycorrhiza fungi and Bradyrhizobium play a vital role in the improvement of soybean protein and oil. Therefore, this study aimed to evaluate the effects of different drought levels and inoculation/non-inoculation with mycorrhiza fungi and Bradyrhizobium on the soybean oil, protein percentage, and fatty acid composition.
Methods: This experiment was conducted a split-plot factorial layout based on a randomized complete block design with three replications in 2017. The main factor was irrigation after 70, 110, and 150 mm of evaporation from a class A evaporation pan. Subplots included mycorrhiza fungal inoculations with Funneliformis mosseae, Rhizophagus intraradices, and without fungus, and bacterium inoculation at two levels (Bradyrhizobium japonicum and without bacteria).
Results: Drought stress significantly reduced the oil percentage. The highest oil content (21.94%) was observed in F. mosseae mycorrhiza inoculation. The highest amount of unsaturated fatty acid (74.75%) was observed after inoculation with R. intraradices and bacteria under full irrigation conditions. Inoculation with mycorrhiza fungi and bacteria increased the unsaturated fatty acids in some cases.
Conclusion: In conclusion, to improve the oil and protein percent, enhance unsaturated fatty acids, and reduce saturated fatty acids in the soybean, the application of mycorrhiza fungi and inoculation with B. japonicum may be beneficial.
 

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Main Subjects

References

Abhari A, Gholinezhad E. 2019. Effects of humic acid on grain yield and yield components in chickpea under different irrigation levels. J Plant Physiol Breed. 9(2): 19-29. https://doi.org/10.22034/jppb.2019.10441
Allison LE, Moodie CD. 1965. Carbonates.  In: Black (Ed.). Methods of soil Analysis. Part 2. WI, USA: American Society of Agronomy, https://doi.org/10.2134/agronmonogr9.2.c40
AOAC. 2000. AOAC Official Method 969.33. 1: Fatty acids in oils and fats. Preparation of methylesters. Boron trifluoride method. Official Methods of Analysis of the Association of Official Analytical Chemists. 17th ed. Arlington, VA: Association of Official Analytical Chemists.
Ashwin R, Bagyaraj DJ, Mohan Raju B. 2023. Ameliorating the drought stress tolerance of a susceptible soybean cultivar, MAUS 2 through dual inoculation with selected rhizobia and AM fungus. Fungal Biol Biotechnol. 10(1):10. https://doi.org/10.1186/s40694-023-00157-y
Barthet VJ, Chornick T, Daun JK. 2002. Comparison of methods to measure the oil contents in oilseeds. J Oleo Sci. 51(9): 589-597. https://doi.org/10.5650/jos.51.589
Bellaloui N, Mengistu A, Kassem MA. 2013. Effects of genetics and environment on fatty acid stability in soybean seed. Food Sci Nutr. 4(9): 165-175. https://doi.org/10.4236/fns.2013.49A1024
Boyer JS. 1982. Plant productivity in the presence of water deficits. Annu Rev Plant Physiol. 33(1): 251-274. https://doi.org/10.1126/science.218.4571.443
Bouyoucos GJ. 1962. Hydrometer method improved for making particle size analyses of soils. Agron J. 54(5): 464-465. https://doi.org/10.2134/agronj1962.00021962005400050028x
Bremner JM. 1996. Nitrogen‐total. Methods of soil analysis: Part 3 Chemical methods 5: 1085-1121. https://doi.org/10.2136/sssabookser5.3.c37
Divsalar M, Tahmasebi Sarvestani Z, Modaress Sanavi SAM, Hamidi I. 2017. Study the effect of drought stress on oil percent, protein percent and fatty acids composition of soybean grain. J Plant Ecophysiol. 8(27): 44-55 (In Persian with English bstract).
Ezzati Lotfabadi Z, Weisany W, Abdul-Razzak Tahir N, Mohammadi Torkashvand A. 2022. Arbuscular mycorrhiza fungi species improve the fatty acids profile and nutrients status of soybean cultivars grown under drought stress. J Appl Microbiol. 132: 2177–2188. https://doi.org/10.1111/jam.15326.
Fazlara A. 2009. Chemical control of food products. Agricultural Research, Education and Extension Organization publication, 260 pp. (In Persian).
Haluschak P. 2006. Laboratory methods of soil analysis. Canada-Manitoba Soil Survey: 3-133. https://doi.org/10.2136/sssabookser5.1.2ed
Hasanzadeh Ghorttapeh A, Javadi H. 2016. Study on the effects of inoculation with biofirtilizers (Azotobacter and Azospirillum) and nitrogen application on oil, yield and yield components of spring canola in West Azerbaijan. J Crop Prod Proc. 5(18): 39-50 (In Persian with English bstract). https://doi.org/10.18869/acadpub.jcpp.5.18.39
Heshmati S, Amini Dehaghi M, Fathi Amirkhiz K. 2007. Effects of biological and chemical phosphorous fertilizer on grain yield, oil seed and fatty acids of spring safflower in water deficit conditions. Iran J  Field Crop Sci. 48(1): 159-169 (In Persian with English abstract). https://doi.org/10.22059/ijfcs.2017.135975.653977
Igiehon NO, Babalola OO, Cheseto X, Torto B. 2021. Effects of rhizobia and arbuscular mycorrhiza fungi on yield, size distribution and fatty acid of soybean seeds grown under drought stress. Microbiol Res. 242: 126640. https://doi.org/10.1016/j.micres.2020.126640
Kieft TL, Wilch E, O'connor K, Ringelberg DB, White DC. 1997. Survival and phospholipid fatty acid profiles of surface and subsurface bacteria in natural sediment microcosms. Appl Environ Microbiol. 63(4): 1531-42. https://doi.org/10.1128/aem.63.4.1531-1542.1997
Le DT, Nishiyama R, Watanabe Y, Tanaka M, Seki M, Ham LH, Yamaguchi-Shinozaki, K, Shinozaki K, Tran LSP. 2012. Differential gene expression in soybean leaf tissues at late developmental stages under drought stress revealed by genome-wide transcriptome analysis. PloS One 7(11): e49522. https://doi.org/10.1371/journal.pone.0049522
Liu X, Jin J, Wang G, Herbert S. 2008. Soybean yield physiology and development of high-yielding practices in northeast China. Field Crops Res. 105(3): 157-171. https://doi.org/10.1016/j.fcr.2007.09.003
Lobell DB, Schlenker W, Costa-Roberts J. 2011. Climate trends and global crop production since 1980: a review of recent findings. Sci Adv. 333(6042): 616-620. https://doi.org/10.1126/science.1204531
Mesquita C, Hanna M, Costa N. 2007. Crop and harvesting operation characteristics affecting physiological qualities of soybeans-Part II. Appl Eng Agric. 23(4): 433-438. https://doi.org/10.13031/2013.23477
Navabpour S, Hezarjaribi E, Mazandarani A. 2017. Evaluation of drought stress effects on important agronomic traits, protein and oil content of soybean genotypes. Environ Stresses Crop Sci. 10(4): 491-503. (In Persian with Enghlish abstract). https://doi.org/10.22077/escs.2017.61.1021
Nosheen A, Bano A, Ullah F. 2013. The role of plant growth promoting rhizobacteria on oil yield and biodiesel production of canola (Brassica napus L.). Energy Sources, Part A: Recovery, Utilization, and Environmental Effects. 35(16): 1574-1581. https://doi.org/10.1080/15567036.2010.529561
Olsen SR, Cole CV, Watanabe FS. 1954. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. USDA Circular No. 939. Washington DC: US Government Printing Office.
Purdehgan M, Modarres-Sanavy SAM, Ghanaty F, Karami S. 2015. The effect of hegxaconazol on quantitative and qualitative characteristics of two soybean cultivars under water deficit stress. J Crop Prod Proc. 5(16): 109-121 (In Persian with Enghlish abstract).  https://doi.org/10.18869/acadpub.jcpp.5.16.109
Rostami Ajirloo AA, Amiri E. 2022. Effects of nano-potassium fertilizer on yield and water use efficiency of soybean under water deficit conditions (case study: Moghan plain, Iran). Commun Soil Sci Plant Anal. 53(12): 1542-51. https://doi.org/10.1080/00103624.2022.2060247
Sehgal A, Sita K, Siddique KHM, Kumar R, Bhogireddy S, Varshney RK, Hanumantha Rao, B, Nair RM, Prasad PV, Nayyar H. 2018. Drought or/and heat-stress effects on seed filling in food crops: impacts on functional biochemistry, seed yields, and nutritional quality. Front Plant Sci. 9(1705). https://doi.org/10.3389/fpls.2018.01705
Seyed Sharifi R. 2016. Oil Seeds. Ardabil Jahad Daneshgahi Press, Ardabil,Iran, 282 pp. (In Persian).
Sheteiwy MS, Ali DF, Xiong YC, Brestic M, Skalicky M, Hamoud YA, Ulhassan Z, Shaghaleh H, AbdElgawad H, Farooq M, et al. 2021. Physiological and biochemical responses of soybean plants inoculated with Arbuscular mycorrhiza fungi and Bradyrhizobium under drought stress. BMC Plant Biol. 21: 195. https://doi.org/10.1186/s12870-021-02949-z.
Smith SE, Read DJ. 2010. Mycorrhizal symbiosis. Third edition. Academic Press.
Sparks DL, Page A, Helmke P, Loeppert R, Soltanpour P, Tabatabai M, Johnston CT, Sumner ME (Eds.). 1996. Methods of soil analysis. Part 3- Chemical methods. Wisconsin:  Soil Science Society of America Inc. https://doi.org/10.2136/sssabookser5.3
Srivastava S, Yadav S. 2024. A positive role of polyunsaturated fatty acids on sustainable crop production against salt stress: an overview. Biologia 79: 1599-1610. https://doi.org/10.1007/s11756-024-01644-2
Thomas GW, Hargrove WL. 1984. The chemistry of soil acidity. In F. Adams (ed.). Soil acidity and liming. Madison, WI, USA: American Society of Agronomy, Crop Science Society of America, Soil Science Society of America, pp 3–56. https://doi.org/10.2134/agronmonogr12.2ed.c16
Tohidi-Moghaddam HR, Zahedi H, Ghooshchi F. 2011. Oil quality of canola cultivars in response to water stress and super absorbent polymer application. Pesq Agropec Trop Goiânia. 41: 579-586. https://doi.org/10.5216/pat.v41i4.13366
Vatan Doost H, Seyed Sharifi R, Farzaneh S, Hassan Panah D. 2018. Grain filling and some fatty acids composition of canola (Brassica napus L.) with application of bio-fertilizers and irrigation withholding. J Agric Sci Sustain Prod. 27(4): 23-37 (In Persian with Enghlish abstract). 
Vessey JK. 2003. Plant growth promoting rhizobacteria as biofertilizers: Advances and challenges. Plant Soil. 255: 571-586. https://doi.org/10.1023/A:1026037216893
Walkley A, Black IA. 1934. An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Sci. 37(1): 29-38. https://doi.org/10.1097/00010694-193401000-00003
Wang X, Wu Z, Zhou Q, Wang X, Song S, Dong S. 2022. Physiological response of soybean plants to water deficit. Front Plant Sci. 12: 809692.‏ https://doi.org/10.3389/fpls.2021.809692.
Zarei Gh, Shamsi H, Dehghani SM. 2010. The effect of drought stress on yield, yield components and seed oil content of three autumnal rapeseed cultivars (Brassica napus L.). J Res Agric Sci. 6(10): 29-36.  
 
 
Journal of Plant Physiology and Breeding
Volume 14, Issue 1
June 2024
Pages 125-146

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