In vitro propagation of Physalis alkekengi using axillary buds

Document Type : Research Paper

Authors

Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran

Abstract

Chinese lantern (Physalis alkekengi) is known as an important medical herb due to its physalin. This plant is widely used to treat various diseases. Therefore, developing a repeatable micropropagation method for this plant would be valuable. In this study, shoot tips and axillary buds of Chinese lantern were treated with different growth regulators (IAA, NAA, BAP, GA3, and IBA) with various concentrations. In another experiment, the effect of salt concentration of the MS medium was investigated. The results showed that using axillary bud as an explant in the full-strength MS supplemented with 2 mg/L BAP and 0.5 mg/L IAA gave the highest number of shoots per explant (3±1.22), number of nods (13±1.22), and stem length (8.04±1.92).   Using the ¼MS medium supplemented with 2 mg/L BAP and 0.5 mg/L IAA produced the highest number of shoots per explant (3±0.43), number of nodes (4.8±0.45), and stem length (4.97±0.77). A higher number of roots (5) were produced in 30 days on the MS basal medium supplemented with 1 and 2 mg/L NAA. The well-rooted plantlets were isolated and transplanted to the paper cup for hardening and the well-established plants were transferred to the field for acclimatization.
 

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Journal of Plant Physiology and Breeding

Articles in Press, Accepted Manuscript
Available Online from 01 May 2024

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