In vitro propagation of Physalis alkekengi L. using axillary buds

Document Type : Research Paper

Authors

Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran

Abstract

Objective: Chinese lantern (Physalis alkekengi L.) is an important medical herb due to its physalin. This plant is widely used to treat various diseases. Therefore, developing a repeatable micropropagation method for this plant would be valuable.
Methods: In this study, shoot tips and axillary buds of Chinese lantern were treated with different growth regulators (IAA, NAA, BAP, GA3, and IBA) with various concentrations. In another experiment, the effect of salt concentration on the MS medium was investigated.
Results: The results showed that using axillary buds as an explant in the full-strength MS, supplemented with 2 mg/L BAP and 0.5 mg/L IAA, resulted in a higher number of shoots per explant (3±1.22) and the highest number of nodes (13±1.22) and stem length (8.04±1.92). Compared with other strengths of MS salts, the full-strength MS medium, supplemented with 2 mg/L BAP and 0.5 mg/L IAA, or 0.5 mg/L BAP alone produced the highest number of shoots per explant, number of nodes, and stem length. However, the MS salt supplemented with a low concentration of BAP (0.5 mg/L) alone looks economically feasible. Decreasing the salt concentration of the basal medium decreased all micropropagation characteristics and in most cases, the decrease was statistically significant. The ddition of GA3 to the combination of 2 mg/L BAP and 0.5 mg/L IAA, significantly improved stem elongation at the concentration range of 0.5 – 1.5 mg/L The number of roots was higher (2±00) after 30 days on the MS basal medium when supplemented with 1 and 2 mg/L NAA or 2 mg/L IAA. The well-rooted plantlets were isolated and transplanted to paper cups for hardening and the well-established plants were transferred to the field for acclimatization.
Conclusion: In conclusion, our results showed that the full strength of MS medium, supplemented with 2 mg/L BAP plus 0.5 mg/L IAA and 0.5–1.5 mg/L GA3 improved micropropagation characteristics of P. alkekengi.
 

Keywords

Main Subjects

References

Afroz R, Hanaki K, Hasegawa-Kurisu K. 2009. Willingness to pay for waste management improvement in Daka city. Bangladesh. J Environ Manage. 90: 492-502. https://doi.org/10.1016/j.jenvman.2007.12.012
Ahmadi R, Shahhoseini R, Hakim L. 2022.  Micropropagation of Iranian native oregano (Origanum vulgare L.) using growth regulators. J Plant Physiol Breed. 12: 105-116. https://doi.org/10.22034/JPPB.2022.16376
Behrens J. 2004. Herbal harvest with future: Towards sustainable sources for medicinal plants. In: Vines G (ed.). UK: Plantlife. https://www.plantlife.org.uk/uk.
de Jesús Romo-Paz F, Folgado R, Delgado-Aceves L, Zamora-Natera J, Portillo L. 2021. Tissue culture of Physalis angulata L.(Solanaceae): techniques for micropropagation and germplasm long-term preservation. Plant Cell, Tissue Organ Cult. 144(1): 73-78. https://doi.org/10.1007/s11240-020-01970-8
Guney E, Menche J, Vidal M, Barab´asi A-L. 2016.  Network-based in silico drug efficacy screening. Nat Commun. 7: 10331. https://doi.org/10.1038/ncomms10331
Helvaci S, Kökdil M, Kawai M, Duran N, Duran A, Güvenç.  2010. Antimicrobial activity of the extracts and physalin D from Physalis alkekengi and evaluation of antioxidant potential of physalin D. Pharm Biol. 48(2): 142-150. https://doi.org/10.3109/13880200903062606
Intzaar S, Akram M, Afrasiab H. 2013. High frequency multiple shoot formation of pygmy groundcherry (Physalis minima L.): An endangered medicinal plant. Int J Agric Biol. 15: 755-760.
Jain N, Babbar S. 2003. Regeneration of 'juvenile' plants of black plum, Syzygium cuminii Skeels, from nodal explants of mature trees. Plant Cell Tissue Organ Cult. 73: 257-263. https://doi.org/10.1023/A:1023039510659
Kumar O, Ramesh S, Tata S. 2015. Establishment of a rapid plant regeneration system in Physalis angulata L. through axillary meristems. Not Sci Biol. 7(4): 471-474. https://doi.org/10.15835/nsb749707
Lima LGB, Montenegro J, Abreu JP, Santos MCB, Nascimento TP, Santos MS, Ferreira AG, Cameron LC, Ferreira MSL, Teodoro AJ. 2020. Metabolite profiling by UPLC-MSE, NMR, and antioxidant properties of Amazonian fruits: Mamey Apple (Mammea americana), Camapu (Physalis angulata), and Uxi (Endopleura uchi). Molecules. 25(2): 342. https://doi.org/10.3390/molecules25020342
Manzoor A, Ahmad T, Ajmal BM, Hafiz AI, Silvestri C. 2019. Studies on colchicine induced chromosome doubling for enhancement of quality traits in ornamental plants. Plants. 8: 194-202. doi: https://doi.org/10.3390/plants8070194
Mascarenhas LMS, de Santana JRF, Brito AL. 2019. Micropropagation of Physalis peruviana L. Pesqui Agropecu Trop. 49(4). https://doi.org/10.1590/1983-40632019v4955603
Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant. 15(3): 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Padmapriya H, Karthikeyan AVP, Jahir Hussain G, Karthi. C 2011. An efficient protocol for in vitro propagation of Solanum nigrum L. from nodal explants. J Agric Technol. 7(4): 1063-1073.
Raj RP, Glint VD, Nirmal Babu K. 2015. In vitro plant regeneration in Capsicum chinense Jacq (Naga Chili). J Appl Biol Biotechnol. 3(1): 30‐33. https://doi.org/10.7324/JABB.2015.3106
Ramar K, Ayyadurai V, Arulrakash T. 2014. In vitro shoot multiplication and plant regeneration of Physalis peruviana L. an important medicinal Plant. Int J Curr Microbiol Appl Sci. 3)3): 456-464.
Shariff N, Sudarshana MS, Umesha S, Hairprasad P. 2006. Antimicrobial activity of Rauvolfia tetraphylla and Physalis minima leaf and callus extracts. Afr J Biotechnol. 5(10): 946-950.
Sheeba E, Parvathi S, Palanivel S. 2010. Direct regeneration from leaves and node explants of Physalis minima Linn. Eur J Appl Sci. 2(2): 58-61.
Tarinejad A, Amiri S. 2019. Influence of plant growth regulators, carbohydrate source and concentration on micropropagation and other physiological traits of grape (Vitis vinifera L. cv. Shahroudi) under in vitro conditions. J Plant Physiol Breed. 9: 75-89. https://doi.org/10.22034/jppb.2019.10378
Velayutham P, Jahir Hussain G, Baskaran P. 2005. In vitro plantlet formation from nodal explants of Plumbago zeylanica L. – An important medicinal plant. J Swamy Bot Cl. 22: 117-120.
Velayutham P, Karthi C, Nalini P, Jahirhussain G. 2012. In vitro regeneration and mass propagation of Hybanthus enneaspermus (L.) F. Muell. from the stem explants through callus culture. J Agric Technol. 8(3): 1119-1128.
Vengadesan G, Ganapathi A, Amutha S, Selvaraj N. 2002. In vitro propagation of Acacia species: A review. Plant Sci. 163(4): 663-671. https://doi.org/10.1016/S0168-9452(02)00144-9
Vila S, Scocchi A, Mroginski L. 2002. Plant regeneration from shoot apical meristems of Melia azedarach L. (Meliaceae). Acta Physiol Plant. 24: 195–199. https://doi.org/10.1007/s11738-002-0011-y
Zhang K, Zhang Y, Walck J, Tao J. 2019. Non-deep simple morphophysiological dormancy in seeds of Angelica keiskei (Apiaceae). Sci Hortic. 255: 202-208. https://doi.org/10.1016/j.scienta.2019.05.039
Zhou LG, Wu JY. 2006. Development and application of medicinal plant tissue cultures for production of drugs and herbal medicinals in China. Nat Prod Rep. 23: 789-810. https://doi.org/10.1039/b610767b
Journal of Plant Physiology and Breeding
Volume 14, Issue 1
June 2024
Pages 67-76

Files

Share

How to cite

Statistics