Different response of GTP cyclohydrolase I gene from grape under abiotic stresses

Document Type : Research Paper


1 Department of Biotechnology Engineering, Faculty of Agricultural and Natural Resources, Imam Khomeini International University, Qazvin, Iran

2 Department of Biotechnology Engineering, Faculty of Agricultural and Natural Resources, Imam Khomeini International ‎University, Qazvin, Iran‎


Folates are a vital necessity for the retention of normal cellular activity. In contrast to humans, other organisms including plants gain folate coenzymes via de novo synthesis. GTP cyclohydrolase I (gtpch I, EC catalyzes the first step of the biosynthesis of tetrahydrofolate (FH4) in plants by the conversion of GTP to dihydroneopterin triphosphate and formic acid. In this research, the expression pattern of the Vvgtpch I gene was assayed in different organs of the grape by the semi-quantitative RT-PCR. The analyses demonstrated that the Vvgtpch I gene was expressed in all grape organs. The highest amounts of expression were obtained in berry and leaf, whereas the lowest amount of Vvgtpch I transcript was related to the cluster. The response of Vvgtpch I gene to abiotic stresses was also investigated under the alkali and cold stresses by the semi-quantitative RT-PCR. Under the alkali stress, the transcript level of Vvgtpch I gene decreased considerably. Similar to the alkali stress, the transcript level of Vvgtpch I gene decreased under cold stress as well. To analyze the Vvgtpch I gene expression under oxidative stress, different treatments were applied such as chemical inducers, heavy metals, and plant growth regulators to trigger the production of reactive oxygen species. The Vvgtpch I showed a strong increase and a moderate increase in the transcript amount with Cu2+ and H2O2, respectively. Whereas, its transcript level was relatively down-regulated by the heavy metals and hormonal treatments, and almost disappeared by diamide.


Main Subjects

Article Title [Persian]

پاسخ متفاوت ژن GTP سیکلوهیدرولاز I تحت تنش های غیرزنده در انگور

Authors [Persian]

  • نادیا اسلامی بجنوردی 1
  • رحیم حداد 1
  • قاسمعلی گروسی 1
  • رضا حیدری جاپلقی 2
1 گروه مهندسی بیوتکنولوژی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین‌المللی امام خمینی (ره)، قزوین
2 گروه مهندسی بیوتکنولوژی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین‌المللی امام خمینی (ره)، قزوین
Abstract [Persian]

فولات یک نیاز ضروری برای حفظ فعالیت طبیعی سلول به شمار می­ رود. برخلاف انسان، بسیاری از موجودات زنده مانند گیاهان توانایی ساخت این کوآنزیم را دارند. آنزیم GTP سیکلوهیدرولاز I (gtpch I, EC اولین مرحله بیوسنتز تتراهیدروفولات (FH4) در گیاهان را از طریق تبدیل GTP به دی ­هیدرونئوپترین تری­فسفات و فرم یک اسید کاتالیز می­ کند. در این پژوهش، الگوی بیان ژن Vvgtpch I در بافت ­های مختلف انگور با روش RT-PCR نیمه­ کمی مورد بررسی قرار گرفت. نتایج نشان داد که ژن Vvgtpch I در تمام بافت­ های مورد مطالعه بیان می­ شود. بالاترین سطح بیان در بافت­ های حبه و برگ مشاهده شد. در حالی که بافت خوشه کمترین میزان سطح رونوشت را نشان داد. پاسخ ژن Vvgtpch I به تنش­ های غیرزنده مانند تنش­ های قلیایی و سرما با استفاده از روش RT-PCR نیمه­ کمی نیز مورد بررسی قرار گرفت. تحت تنش قلیایی، سطح رونوشت ژن Vvgtpch I به طور چشمگیری کاهش یافت. مشابه با تنش قلیایی، میزان بیان ژن Vvgtpch I نیز تحت تنش سرما با کاهش مواجه شد. به منظور بررسی بیان ژن Vvgtpch I تحت تنش اکسیداتیو، تیمارهای مختلفی شامل القاءکننده­ های شیمیایی، فلزات سنگین و تنظیم­ کننده­ های رشد گیاهی جهت القاء تولید گونه ­های فعال اکسیژن مورد استفاده قرار گرفتند. سطح رونوشت ژن Vvgtpch I تحت تیمار با  +CU2 و H2O2 به ترتیب با افزایش شدید و نسبتاً شدید روبرو شد. با وجود این، سطح رونوشت ژن Vvgtpch I تحت تیمار با فلزات سنگین و هورمون­ های گیاهی نسبتاً کاهش یافت و تحت تیمار با Diamide با کاهش بسیار شدید مواجه شد.

Keywords [Persian]

  • بیان ژن
  • تنش اکسیداتیو
  • تنش غیرزنده
  • فولات
  • گونه‌های فعال اکسیژن
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