Different response of GTP cyclohydrolase I gene from grape under abiotic stresses

Document Type : Research Paper

Authors

1 Department of Biotechnology Engineering, Faculty of Agricultural and Natural Resources, Imam Khomeini International University, Qazvin, Iran

2 Department of Biotechnology Engineering, Faculty of Agricultural and Natural Resources, Imam Khomeini International ‎University, Qazvin, Iran‎

Abstract

Folates are a vital necessity for the retention of normal cellular activity. In contrast to humans, other organisms including plants gain folate coenzymes via de novo synthesis. GTP cyclohydrolase I (gtpch I, EC 3.5.4.16) catalyzes the first step of the biosynthesis of tetrahydrofolate (FH4) in plants by the conversion of GTP to dihydroneopterin triphosphate and formic acid. In this research, the expression pattern of the Vvgtpch I gene was assayed in different organs of the grape by the semi-quantitative RT-PCR. The analyses demonstrated that the Vvgtpch I gene was expressed in all grape organs. The highest amounts of expression were obtained in berry and leaf, whereas the lowest amount of Vvgtpch I transcript was related to the cluster. The response of Vvgtpch I gene to abiotic stresses was also investigated under the alkali and cold stresses by the semi-quantitative RT-PCR. Under the alkali stress, the transcript level of Vvgtpch I gene decreased considerably. Similar to the alkali stress, the transcript level of Vvgtpch I gene decreased under cold stress as well. To analyze the Vvgtpch I gene expression under oxidative stress, different treatments were applied such as chemical inducers, heavy metals, and plant growth regulators to trigger the production of reactive oxygen species. The Vvgtpch I showed a strong increase and a moderate increase in the transcript amount with Cu2+ and H2O2, respectively. Whereas, its transcript level was relatively down-regulated by the heavy metals and hormonal treatments, and almost disappeared by diamide.

Keywords

Main Subjects

Article Title [Persian]

پاسخ متفاوت ژن GTP سیکلوهیدرولاز I تحت تنش های غیرزنده در انگور

Authors [Persian]

  • نادیا اسلامی بجنوردی 1
  • رحیم حداد 1
  • قاسمعلی گروسی 1
  • رضا حیدری جاپلقی 2
1 گروه مهندسی بیوتکنولوژی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین‌المللی امام خمینی (ره)، قزوین
2 گروه مهندسی بیوتکنولوژی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین‌المللی امام خمینی (ره)، قزوین
Abstract [Persian]

فولات یک نیاز ضروری برای حفظ فعالیت طبیعی سلول به شمار می­ رود. برخلاف انسان، بسیاری از موجودات زنده مانند گیاهان توانایی ساخت این کوآنزیم را دارند. آنزیم GTP سیکلوهیدرولاز I (gtpch I, EC 3.5.4.16) اولین مرحله بیوسنتز تتراهیدروفولات (FH4) در گیاهان را از طریق تبدیل GTP به دی ­هیدرونئوپترین تری­فسفات و فرم یک اسید کاتالیز می­ کند. در این پژوهش، الگوی بیان ژن Vvgtpch I در بافت ­های مختلف انگور با روش RT-PCR نیمه­ کمی مورد بررسی قرار گرفت. نتایج نشان داد که ژن Vvgtpch I در تمام بافت­ های مورد مطالعه بیان می­ شود. بالاترین سطح بیان در بافت­ های حبه و برگ مشاهده شد. در حالی که بافت خوشه کمترین میزان سطح رونوشت را نشان داد. پاسخ ژن Vvgtpch I به تنش­ های غیرزنده مانند تنش­ های قلیایی و سرما با استفاده از روش RT-PCR نیمه­ کمی نیز مورد بررسی قرار گرفت. تحت تنش قلیایی، سطح رونوشت ژن Vvgtpch I به طور چشمگیری کاهش یافت. مشابه با تنش قلیایی، میزان بیان ژن Vvgtpch I نیز تحت تنش سرما با کاهش مواجه شد. به منظور بررسی بیان ژن Vvgtpch I تحت تنش اکسیداتیو، تیمارهای مختلفی شامل القاءکننده­ های شیمیایی، فلزات سنگین و تنظیم­ کننده­ های رشد گیاهی جهت القاء تولید گونه ­های فعال اکسیژن مورد استفاده قرار گرفتند. سطح رونوشت ژن Vvgtpch I تحت تیمار با  +CU2 و H2O2 به ترتیب با افزایش شدید و نسبتاً شدید روبرو شد. با وجود این، سطح رونوشت ژن Vvgtpch I تحت تیمار با فلزات سنگین و هورمون­ های گیاهی نسبتاً کاهش یافت و تحت تیمار با Diamide با کاهش بسیار شدید مواجه شد.

Keywords [Persian]

  • بیان ژن
  • تنش اکسیداتیو
  • تنش غیرزنده
  • فولات
  • گونه‌های فعال اکسیژن

References

Agyenim-Boateng KG, Zhang S, Shohag MJI, Shaibu AS, Li J, Li B, Sun J. 2023a. Folate biofortification in soybean: challenges and prospects. Agronomy. 13(1): 241.
Agyenim-Boateng KG, Zhang S, Zhang S, Khattak AN, Shaibu AS, Abdelghany AM, Qi J, Azam M, Ma C, Feng Y, et al. 2023b. The nutritional composition of the vegetable soybean (Maodou) and its potential in combatting malnutrition. Front Nutr. 9: 1034115.
Ashokkumar K, Govindaraj M, Karthikeyan A, Shobhana VG, Warkentin TD. 2020. Genomics-integrated breeding for carotenoids and folates in staple cereal grains to reduce malnutrition. Front Genet. 11: 414.
Ashokkumar K, Sivakumar P, Saradhadevi M. 2018. Identification and determination of naturally occurring folates in grains of rice (Oryza sativa L.) by UPLC-MS/MS analysis. Nat Prod Res. 32(14): 1733–1737.

Aune D, Deneo-Pellegrini H, Ronco AL, Boffetta P, Acosta G, Mendilaharsu M, De Stefani E. 2011. Dietary folate intake and the risk of 11 types of cancer: a case–control study in Uruguay. Ann Oncol. 22(2): 444–451.

Baena-Gonzalez E, Rolland F, Thevelein JM, Sheen J. 2007. A central integrator of transcription networks in plant stress and energy signalling. Nature. 448(7156): 938–942.
Basset G, Quinlivan EP, Ziemak MJ, Diaz De La Garza R, Fischer M, Schiffmann S, Bacher A, Gregory JF, Hanson AD. 2002. Folate synthesis in plants: the first step of the pterin branch is mediated by a unique bimodular GTP cyclohydrolase I. Proc Natl Acad Sci USA. 99(19): 12489–12494.
Blancquaert D, De Steur H, Gellynck X, Van Der Straeten D. 2014. Present and future of folate biofortification of crop plants. J Exp Bot. 65(4): 895–906.
Blau N, van Spronsen FJ. 2014. Disorders of phenylalanine and tetrahydrobiopterin metabolism. In: Blau N, Duran M, Gibson K, Dionisi Vici C (eds). Physician's guide to the diagnosis, treatment, and follow-up of inherited metabolic diseases. Springer: Berlin, pp. 3–21.
Blokhina O, Virolainen E, Fagerstedt KV. 2003. Antioxidants, oxidative damage and oxygen deprivation stress. Ann Bot. 91(2): 179–194.
Changsong Z, Diqiu YU. 2010. Analysis of the cold-responsive transcriptome in the mature pollen of Arabidopsis. J Plant Biol. 53: 400–416.
Chavan B, Beazley W, Wood JM, Rokos H, Ichinose H, Schallreuter KU. 2009. H2O2 increases de novo synthesis of (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin via GTP cyclohydrolase I and its feedback regulatory protein in vitiligo. J Inherit Metab Dis. 32: 86–94.
Chinnusamy V, Zhu J, Zhu JK. 2007. Cold stress regulation of gene expression in plants. Trends Plant Sci. 12(10): 444–451.
De Lepeleire J, Strobbe S, Verstraete J, Blancquaert D, Ambach L, Visser RGF, Stove C, van Der Straeten D. 2018. Folate biofortification of potato by tuber-specific expression of four folate biosynthesis genes. Mol Plant. 11(1): 175–188.
Diaz ML, Soresi DS, Basualdo J, Cuppari SJ, Carrera A. 2019. Transcriptomic response of durum wheat to cold stress at reproductive stage. Mol Biol Rep. 46: 2427–2445.
Diaz De la Garza RI, Gregory III JF, Hanson AD. 2007. Folate biofortification of tomato fruit. Proc Natl Acad Sci USA. 104(10): 4218–4222.
Eslami-Bojnourdi N, Haddad R, Garousi GA, Navabpour S, 2017. Expression analysis of gtp cyclohydrolase I gene under abiotic stresses in grape (Vitis vinifera L.). Mod Genet J. 11(4): 539–546 (In Persian with English abstract).
Gong B, Wen D, VandenLangenberg K, Wei M, Yang F, Shi Q, Wang X. 2013. Comparative effects of NaCl and NaHCO3 stress on photosynthetic parameters, nutrient metabolism, and the antioxidant system in tomato leaves. Sci Hortic. 157: 1–12.
Gupta S, Singh Y, Kumar H, Raj U, Rao AR, Varadwaj PK. 2016. Identification of novel abiotic stress proteins in Triticum aestivum through functional annotation of hypothetical proteins. Interdiscip Sci Comput Life Sci. 10: 205–220.
Heidari-Japelaghi R, Haddad R, Garoosi GA. 2011. Rapid and efficient isolation of high quality nucleic acids from plant tissues rich in polyphenols and polysaccharides. Mol Biotechnol. 49: 129–137.
Imbard A, Benoist JF, Blom HJ. 2013. Neural tube defects, folic acid and methylation. Int J Environ Res Public Health. 10(9): 4352–4389.
Kamal AHM, Kim KH, Shin KH, Choi JS, Baik BK, Tsujimoto H, Heo HY, Park C-S, Woo S-H. 2010. Abiotic stress responsive proteins of wheat grain determined using proteomics technique. Aust J Crop Sci. 4(3): 196–208.
Kim DH, Kang WH, Yeom SI, Kim BD. 2019. Isolation of putative pepper defense‑related genes against the pathogen Phytophthora capsiciusing suppression subtractive hybridization/macroarray and RNA-sequencing analyses. Hortic Environ Biotechnol. 60: 685–699.
Liang Q, Wang K, Shariful I, Ye X, Zhang C. 2020. Folate content and retention in wheat grains and wheat-based foods: effects of storage, processing, and cooking methods. Food Chem. 333: 127459.
Mahajan S, Tuteja N. 2005. Cold, salinity and drought stresses: an overview. Arch Biochem Biophys. 444(2): 139–158.
McIntosh SR, Brushett D, Henry RJ, 2008. GTP cyclohydrolase 1 expression and folate accumulation in the developing wheat seed. J Cereal Sci. 48(2): 503–512.
Navarrete O, van Daele J, Stove C, Lambert W, van Der Straeten D, Storozhenko S. 2012. A folate independent role for cytosolic HPPK/DHPS upon stress in Arabidopsis thaliana. Phytochem. 73: 23–33.
Nunes AC, Kalkmann DC, Aragao FJ. 2009. Folate biofortification of lettuce by expression of a codon optimized chicken GTP cyclohydrolase I gene. Transgenic Res. 18(5): 661–667.

Perez-Duenas B, Ormazabal A, Toma C, Torrico B, Cormand B, Serrano M, Sierra C, De Grandis E, Marfa MP, García-Cazorla A, et al. 2011. Cerebral folate deficiency syndromes in childhood clinical, analytical, and etiologic aspects. Arch Neurol. 68(5): 615–621.

Ramirez Rivera NG, Garcia-Salinas C, Aragao FJ, De la Garza RIl. 2016. Metabolic engineering of folate and its precursors in Mexican common bean (Phaseolus vulgaris L.). Plant Biotechnol J. 14(10): 2021–2032.

Schallreuter KU, Elwary S. 2007. Hydrogen peroxide regulates the cholinergic signal in a concentration dependent manner. Life Sci. 80(24-25): 2221–2226.

Shahid M, Lian T, Wan X, Jiang L, Han L, Zhang C, Liang Q. 2020. Folate monoglumate in cereal grains: evaluation of extraction techniques and determination by LC-MS/MS. J Food Compost Anal. 91: 103510.
Shi H, Ishitani M, Kim C, Zhu JK. 2000. The Arabidopsis thaliana salt tolerance gene SOS1 encodes a putative Na+/H+ antiporter. Proc Natl Acad Sci USA. 97(12): 6896–6901.

Storozhenko S, De Brouwer V, Volckaert M, Navarrete O, Blancquaert D, Zhang GF, Lambert W, van Der Straeten D. 2007. Folate fortification of rice by metabolic engineering. Nat Biotechnol. 25(11): 1277–1279.

Strobbe S, van Der Straeten D. 2017. Folate biofortification in food crops. Curr Opin Biotechnol. 44: 202–211.
Wakeel A, Arif S, Bashir MA, Ahmad Z, Rehman HU, Kiran A, Ibrahim S, Khan MR. 2018. Perspectives of folate biofortification of cereal grains. J Plant Nutr. 41(19): 2507-2524.
Waller JC, Akhtar TA, Lara-Nunez A, Gregory JF, McQuinn RP, Giovannoni JJ, Hanson AD. 2010. Developmental and feed forward control of the expression of folate biosynthesis genes in tomato Fruit. Mol Plant. 3(1): 66–77.
Journal of Plant Physiology and Breeding
Volume 13, Issue 2
December 2023
Pages 131-143

Files

Share

How to cite

Statistics