Biometrical analysis of resistance to stem rust (Puccinia graminis f. sp. tritici) in the winter wheat genotypes

Document Type : Research Paper

Authors

1 Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabil, Iran

2 Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran

3 Department of Cereal Research, Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran; Crop and Horticultural Science Research Department, East Azarbaijan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Tabriz, Iran

4 Department of Cereal Research, Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Abstract

Stem rust or black rust is one of the most important fungal diseases that widely affect wheat yield and quality in the world. Therefore, the selection of genetic materials resistant to stem rust in the breeding programs is necessary. In this study, 24 winter wheat genotypes including eight varieties and 16 elite lines were evaluated at the adult plant and seedling stages using a randomized complete block design under the influence of local stem rust race TKTTF. Disease indices including the type of infection, the severity of infection, the coefficient of infection, the area under the disease progress curve (AUDPC), the relative area under the disease progress curve (rAUDPC), and genotype reaction were recorded. Significant differences were observed among the genotypes for all disease indices. Based on all indices, MV-17 and C-98-17 were resistant and C-98-14, C-98-9, Bolany, and Morocco were susceptible. Pearson’s correlation coefficients revealed a significant positive correlation between field type of infection, the severity of infection, the coefficient of infection, AUDPC, and rAUDPC at the field, and between greenhouse type of infection and genotype reaction at the greenhouse. Based on cluster analysis by Ward’s method, all the genotypes were classified into four groups (R, MR, MS, and S) in the adult plant stage and into two groups (R and S) at the seedling stage. The resistant genotypes can be used in the breeding programs for improvement of the stem-rust-resistant genotypes.

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References

Benesty J, Chen J, Huang Y, Cohen I. 2009. Pearson correlation coefficient. In: Benesty J, Chen J, Huang Y, Cohen I, editors. Noise reduction in speech processing. Springer Topics in Signal Processing, vol. 2. Berlin, Heidelberg: Springer.
Burdon JJ. 1993. Genetic variation in pathogen populations and its implications for adaptation to host resistance. Springer. In: Jacobs T, Parlevliet JE, editors. Durability of disease resistance. Current Plant Science and Biotechnology in Agriculture, vol. 18. Dordrecht: Springer. P. 41-56.
Dadrezaei S, Afshari F, Patpour M. 2015. Evaluation of phenotypic resistance to rusts in some Iranian wheat genotypes in greenhouse and field conditions. Seed Plant J. 31)3(: 531-546 (In Persian with English abstract).
Degete AG, Chala A. 2019. Effects of stem rust (Puccinia graminis f. sp. tritici) on yield, physical and chemical quality of durum wheat varieties in East Shoa Zone, Ethiopia. Am J Agric For. 7: 78-83.
Denbel W, Badebo A, Alemu T. 2013. Evaluation of Ethiopian commercial wheat cultivars for resistance to stem rust of wheat race ‘UG99’. Int J Agron Plant Prod. 4: 15-24.
Ellis JG, Lagudah ES, Spielmeyer W, Dodds PN. 2014. The past, present and future of breeding rust resistant wheat. Front Plant Sci. 5: 641-650.
Ghazvini H, Hiebert CW, Zegeye T, Fetch T. 2012. Inheritance of stem rust resistance derived from Aegilops triuncialis in wheat line Tr129. Can J Plant Sci. 92: 1037-1041.
Hiebert CW, Fetch TG, Zegeye T. 2010. Genetics and mapping of stem rust resistance to Ug99 in the wheat cultivar. Theor App Genet. 121: 65-69.
Hiebert CW, Kassa MT, McCartney CA, You FM, Rouse MN, Fobert P, and Fetch TG. 2016. Genetics and mapping of seedling resistance to Ug99 stem rust in winter wheat cultivar Triumph 64 and differentiation of SrTmp, SrCad, and Sr42. Theor App Genet. 129: 2171-2177.
Hundie B, Yirga F, Kassa D, Hailu E, Negash T, Tesfaye T, Bacha N, Shewaye Y, Woldeab G, Zegaye H, et al. 2018. Evaluation of advanced bread wheat lines for field and seedling resistance to stem rust (Puccinia graminis f. sp. tritici). Am J Biol Environ Stat. 4: 74-82.
Jin Y, Singh R, Ward R, Wanyera R, Kinyua M, Njau P, Fetch T, Pretorius Z, Yahyaoui A. 2007. Characterization of seedling infection types and adult plant infection responses of monogenic Sr gene lines to race TTKS of Puccinia graminis f. sp. tritici. Plant Dis. 91: 1096-1099.
Jin Y, Szabo L, Pretorius Z, Singh R, Ward R, Fetch Jr T. 2008. Detection of virulence to resistance gene Sr24 within race TTKS of Puccinia graminis f. sp. tritici. Plant Dis. 92: 923-926.
Jin Y, Szabo L, Rouse M, Fetch Jr T, Pretorius Z, Wanyera R, Njau P. 2009. Detection of virulence to resistance gene Sr36 within the TTKS race lineage of Puccinia graminis f. sp. tritici. Plant Dis. 93: 367-370.
Johnson R, 1983. Genetic background of durable resistance. In: Lamberti F, Waller JM, Van der Graaff NA, editors. Durable resistance in crops. NATO Advanced Science Institutes Series, vol. 55. Boston: Springer. p. 5-26.
Knott DR. 2012. The wheat rusts - breeding for resistance. Springer-Verlag: Berlin.
Kolmer JA. 2005. Tracking wheat rust on a continental scale. Curr Opin Plant Biol. 8: 441-449.
Mapuranga J, Zhang N, Zhang L, Liu W, Chang J and Yang W, 2022. Harnessing genetic resistance to rusts in wheat and integrated rust management methods to develop more durable resistant cultivars. Frontiers in Plant Science 13:1-28.
Newcomb M, Olivera PD, Rouse MN, Szabo LJ, Johnson J, Gale S, Luster DG, Wanyera R, Macharia G, Bhavani S. 2016. Kenyan isolates of Puccinia graminis f. sp. tritici from 2008 to 2014: virulence to SrTmp in the Ug99 race group and implications for breeding programs. Phytopathology 106: 729-736.
Olivera P, Newcomb M, Szabo LJ, Rouse M, Johnson J, Gale S, Luster DG, Hodson D, Cox JA, Burgin L. 2015. Phenotypic and genotypic characterization of race TKTTF of Puccinia graminis f. sp. tritici that caused a wheat stem rust epidemic in southern Ethiopia in 2013–14. Phytopathology 105: 917-928.
Park R. 2016. Wheat: biotrophic pathogen resistance. Reference Module in Food Science. Amsterdam, The Netherlands: Elsevier.
Peterson RF, Campbell A, Hannah A. 1948. A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can J Res. 26: 496-500.
Poland J, Rutkoski J. 2016. Advances and challenges in genomic selection for disease resistance. Annu Rev Phytopathol. 54: 79-98.
Pretorius ZA, Szabo LJ, Boshoff WHP, Herselman L, Visser B. 2012. First report of a new TTKSF race of wheat stem rust (Puccinia graminis f. sp. tritici) in South Africa and Zimbabwe. Plant Dis. 96: 590-590.
Roelfs AP. 1985. Wheat and rye stem rust. IN: Roelfs AP, Bushnell WR, editor.  Diseases, distribution, epidemiology, and control. Cambridge, USA: Academic Press.
Roelfs AP, Singh RP, Saari EE. 1992. Rust diseases of wheat: concepts and methods of disease management. CIMMYT. https://repository.cimmyt.org/xmlui/handle/10883/1153?show=full.
Rouse MN, Wanyera R, Njau P, Jin Y. 2011. Sources of resistance to stem rust race Ug99 in spring wheat germplasm. Plant Dis. 95: 762-766.
Salcedo A, Rutter W, Wang S, Akhunova A, Bolus S, Chao S, Anderson N, De Soto MF, Rouse M, Szabo L. 2017. Variation in the AvrSr35 gene determines Sr35 resistance against wheat stem rust race Ug99. Science 358: 1604-1606.
Schwarz T. 2020. Climate-data.org. Available at http://es. climate-data. org/.
Semenov MA, Halford NG. 2009. Identifying target traits and molecular mechanisms for wheat breeding under a changing climate. J Exp Bot. 60(10): 2791-2804.
Singh RP, Hodson DP, Huerta-Espino J, Jin Y, Bhavani S, Njau P, Herrera-Foessel S, Singh PK, Singh S, Govindan V. 2011. The emergence of Ug99 races of the stem rust fungus is a threat to world wheat production. Annu Rev Phytopathol. 49: 465-481.
Soresa DN. 2018. Evaluation of bread wheat (Triticum aestivum L.) genotypes for resistance against stem rust (Puccinia graminis f. sp. tritici) diseases at seedling and adult stages. Afr J Agric Res. 13: 2904-2910.
Stakman EC, Stewart D, Loegering W. 1962. Identification of physiologic races of Puccinia graminis var. tritici. Washington, DC: US Department of Agricultural Publications E617, USDA.
Taye T, Fininsa C, Woldeab G. 2013. Importance of wheat stem rust (Puccinia graminis f. sp. tritici) in Guji zone, Southern Ethiopia. Plant. 2(1): 1-5.
Wanyera R, Kinyua M, Jin Y and Singh R, 2006. The spread of stem rust caused by Puccinia graminis f. sp. tritici, with virulence on Sr31 in wheat in Eastern Africa. Plant Disease 90: 113-113.
Wilcoxson RD, Skovmand B, Atif A. 1975. Evaluation of wheat cultivars for ability to retard development of stem rust. Ann Appl Biol. 80: 275-281.
Woldeab G, Hailu E, Bacha N. 2017. Protocols for race analysis of wheat stem rust (Puccinia graminis f. sp. tritici). Ethiopian Institute of Agricultural Research Ambo Plant Protection Research Center Ambo, Ethiopia. Available online at www. globalrust. org/race-manual/Ambo.
Zhang H, Wang Z, Ren J, Du Z, Quan W, Zhang Y, Zhang Z. 2017. A QTL with major effect on reducing stripe rust severity detected from a Chinese wheat landrace. Plant Dis. 101: 1533-1539.
Journal of Plant Physiology and Breeding
Volume 13, Issue 2
December 2023
Pages 113-130

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