The effect of tetraploidy on the expression of genes involved in alkaloid biosynthesis in Papaver fugax Poir.

Document Type : Research Paper

Authors

1 Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.

2 Meshginshahr Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran.

Abstract

Polyploidy, a common phenomenon in the evolution of plant species, plays an imperative role in plant diversification. Bride rose poppy (Papaver fugax Poir.) is a valuable medicinal plant due to its various alkaloids, such as fugapavin, mecambrin, romerine, salutaridine, protopine, and thebaine. In this study, we used quantitative polymerase chain reaction (qRT-PCR) to study the differential expression of four key genes, including CYPB80B1 ((S)-N-methylcoclaurine-3’-hydroxylase), MLP (major latex protein), 6OMT ((S)-norcoclaurine-6-O-methyltransferase), and salAT (7(S)-salutaridinol-7-O-acetyltransferase), involved in the biosynthesis of poppy alkaloids. The assessment of extracted RNA samples on 1% agarose gel electrophoresis and NanoDrop showed the proper quality and quantity of isolated RNA samples for cDNA synthesis. Gene expression analysis through qRT-PCR revealed increased expression of CYP80B1, MLP, and SalAT genes and decreased expression of 6OMT in tetraploid plants compared with diploids. Our results indicated that autotetraploidy induction could be applied, as a useful method, to increase morphine production in P.  fugax.

Keywords

Main Subjects

Article Title [Persian]

تأثیر تتراپلوئیدی بر بیان ژن‌های دخیل در بیوسنتز آلکالوئید در Papaver fugax Poir.

Authors [Persian]

  • گیتی ستوده اردبیلی 1
  • رسول اصغری ذکریا 1
  • ناصر زارع 1
  • لیلا غفارزاده نمازی 2
1 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل
2 دانشکده کشاورزی مشگین شهر، دانشگاه محقق اردبیلی، اردبیل
Abstract [Persian]

پلی‌پلوئیدی، پدیده‌ای رایج در تکامل گونه­ های گیاهی، نقش مهمی در تنوع گیاهی ایفا می‌کند. خشخاش برگ ریزان (Papaver fugax Poir.)  به دلیل داشتن آلکالوئیدهای مختلف مانند فوگاپاوین، مکامبرین، رومرین، سالوتاریدین، پروتوپین و تبائین، گیاه دارویی ارزشمندی است. در این مطالعه از واکنش زنجیره‌ای پلیمراز کمی (qRT-PCR) برای بررسی بیان افتراقی چهار ژن کلیدی در بیوسنتز آلکالوئیدهای خشخاش {CYPB80B1 ((S)-N-methylcoclaurine-3'-hydroxylase) ،MLP (Major latex protein)  ،OMT6  ((S)-norcoclaurine-6-O-methyltransferase)  و(7(S)-salutaridinol-7-O-acetyltransferase) salAT   استفاده شد. ارزیابی نمونه‌های RNA استخراج‌شده روی الکتروفورز ژل آگارز 1 درصد و نانودراپ، کیفیت و کمیت مناسب نمونه‌های RNA جدا شده برای سنتز cDNA  را نشان داد. تجزیه و تحلیل بیان ژن‌ از طریق qRT-PCR بیانگر افزایش بیان ژن‌های CYP80B1 ،MLP  و SalAT و کاهش بیان ژن  OMT6 در گیاهان تتراپلوئید در مقایسه با دیپلوئیدها بود. این نتایج نشان داد که القای اتوتتراپلوئیدی می‌تواند به عنوان یک روش مفید برای افزایش تولید مورفین در P. fugax استفاده شود.

Keywords [Persian]

  • آلکالوئیدها
  • بیان ژن
  • تتراپلوئیدی
  • Papaver fugax

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Journal of Plant Physiology and Breeding
Volume 13, Issue 2
December 2023
Pages 99-111

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