Assessment of genetic diversity in different types of tobacco using molecular markers

Document Type : Research Paper


1 Researcher, Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.

2 Associate Professor, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.

3 Professor, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.


Information about the amount of genetic diversity in the germplasm and genetic relations of genotypes is essential for analyzing and designing breeding programs and could be used for assisting in genetic identification and improvement of the germplasm. In this research, the genetic diversity of 48 genotypes of the flue-cured, burley, and oriental types of tobacco was investigated by 12 ISSR, 10 SSR, and five IRAP primers. The maximum number of bands was observed in the UBC825, RTR-10, and mixed primer UBC817 + UBC826 with 17 bands, UBC817 with 16 bands, and RTR-8 with 15 bands. UBC824 with 10 and UBC823 with 11 bands showed the minimum number of bands among ISSR and IRAP primers, and PT30044 and PT30046 primers with two alleles showed the minimum number of alleles among SSR markers. In ISSR and IRAP, the observed percentage of polymorphism ranged from 76.92% for RTR-1 and RTR-7 to 94.11% for RTR-10, and in SSR markers, it was 100%. The average polymorphism percentage was 90.7%, which indicated suitable genetic diversity among the tobacco genotypes. The polymorphism information content ranged from 0.31 to 0.5 with an average of 0.42. The average diversity of Nei and Shannon indices were 0.39 and 0.58, respectively. Cluster analysis by the UPGMA method classified the 48 tobacco genotypes into five groups, containing 5, 12, 10, 6, and 15 genotypes, respectively. Principal coordinate analysis showed that the 14 first components could explain 51.49% of the total variance. The primers used in this study had high efficiency, which can be used to study the genetic diversity in tobacco.


Main Subjects

Article Title [Persian]

بررسی تنوع ژنتیکی انواع مختلف توتون از طریق نشانگرهای مولکولی

Authors [Persian]

  • مصطفی مهدی زاده 1
  • محمد محسن زاده گلفزانی 2
  • حبیب ا... سمیع زاده 3
1 محقق اصلاح نباتات، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت
2 دانشیار گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت
3 استاد گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت
Abstract [Persian]

اطلاعات مربوط به میزان تنوع ژنتیکی در ژرم­ پلاسم و روابط ژنتیکی بین ژنوتیپ ­ها برای بررسی و طراحی برنامه­ های به‌نژادی مهم می­ باشد و می‌تواند برای کمک به شناسایی و بهبود ژنتیکی ژرم­ پلاسم به ­کار رود. در این تحقیق، تنوع ژنتیکی 48 ژنوتیپ توتون از تیپ‌های گرمخانه‌ای، بارلی و شرقی توسط 12 نشانگر ISSR، 10 نشانگر SSR و 5 نشانگر IRAP بررسی شد. آغازگرهای UBC825، RTR-10 و آغازگر ترکیبی UBC817 + UBC826 با تعداد 17 نوار و بعد از آن، آغازگر UBC817 با 16 نوار و RTR-8 با 15 نوار بیشترین تعداد نوار و آغازگر UBC824 با 10 و UBC823 با 11 نوار کمترین تعداد نوار در بین آغازگرهای ISSR و IRAP را داشتند. همچنین آغازگرهای PT30044 و PT30046 با 2 نوار از کمترین تعداد نوار در بین آغازگرهای SSR برخوردار بودند. درصد چندشکلی به دست آمده از 76.92 درصد برای RTR-1 و RTR-7 تا 94.11 درصد برای RTR-10 در آغازگرهای ISSR و IRAP متغیر بود و چندشکلی در آغازگرهای SSR صد درصد به دست آمد. میانگین چندشکلی 90.7 درصد، وجود تنوع ژنتیکی مناسب بین ژنوتیپ ­های توتون را نشان می ­دهد. محتوای اطلاعات چندشکلی در این تحقیق بین 0.31 تا 0.5 و میانگین محتوای اطلاعات چندشکلی 0.42 بود. میانگین تنوع ژنی نی و شاخص شانون به ترتیب 0.39 و 0.58 به دست آمد. تجزیه خوشه ­ای به روش UPGMA،  ژنوتیپ­ های توتون مورد بررسی را در پنج گروه قرار داد که به ترتیب شامل 5، 12، 10، 6 و 15 ژنوتیپ شدند. بر اساس تجزیه به بردارهای اصلی 14 مؤلفه اول 51.49 درصد از واریانس کل را توجیه کردند. پرایمرهای مورد استفاده در این مطالعه کارایی بالایی داشتند که می ­توان از آن­ ها برای بررسی تنوع ژنتیکی در توتون استفاده کرد.

Keywords [Persian]

  • بارلی
  • شرقی
  • گرمخانه ای
  • IRAP
  • ISSR
  • SSR
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