Evaluation of morphological traits and chemical compounds of chicory (Cichorium intybus) populations in central Iran (Markazi province)

Document Type : Research Paper

Authors

Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran

Abstract

Chicory has a wide range of uses in the pharmaceutical and food industries owing to its valuable bioactive compounds such as phenolic acids, terpenoids, vitamins, and inulin. The presence and content of these compounds are influenced by genetic and environmental factors, as well as the processing method. The present research aimed to evaluate the morphological and biochemical characteristics of wild chicory populations from the Markazi province, Iran. The essential oil was extracted through the distillation technique using Clevenger-type apparatus. GC and GC/MS were used to identify the compounds of the essential oil, and a spectrophotometer was used to determine the amount of inulin. The results showed high and significant diversity between populations for the measured traits. The maximum and minimum variations were observed for the plant height and branch weight, respectively. Significant correlations were observed between different traits. The yield and composition of essential oil in the studied populations showed considerable variation. The main components of the essential oil were chamazulene, 1,8-cineole, carvacrol, cuminic aldehyde, thymol, cinnamic aldehyde, camphor, borneol, linalool, and carvone. Cluster analysis separated the populations into two different groups based on morphological traits and chemical components of essential oils. In conclusion, the existence of considerable diversity in morphological traits and chemical compounds among the chicory populations can be useful in the breeding programs of this plant.

Keywords


Adams RP, 2007. Identification of essential oil components by gas chromatography/mass spectrometry. 4th Edition. Allured Publ., Carol Stream, IL, USA.
Araus JL and Cairns JE, 2014. Field high-throughput phenotyping: the new crop breeding frontier. Trends in Plant Science 19(1): 52-61.
Bais HP and Ravishankar GA, 2001. Cichorium intybus L. cultivation, processing, utility, value addition and biotechnology, with an emphasis on current status and future prospects. Journal of the Science of Food and Agriculture 81(5): 467-484.
Barcaccia G, Ghedina A, and Lucchin M, 2016. Current advances in genomics and breeding of leaf chicory (Cichorium intybus L.). Agriculture 6(4):1-24.
Baricevic D, Mathe, A, and Bartol T, 2015. Conservation of wild crafted medicinal and aromatic plants and their habitats. In: Máthé Á (ed.). Medicinal and Aromatic Plants of the World. Springer. Pp. 131-144.
Bartels SF and Chen HY, 2013. Interactions between overstorey and understorey vegetation along an overstorey compositional gradient. Journal of Vegetation Science 24(3): 543-552.
Bergonzi MC, Bilia AR, Gallori S, Guerrini D, and Vincieri FF, 2001. Variability in the content of the constituents of Hypericum perforatum L. and some commercial extracts. Drug Development and Industrial Pharmacy 27: 491-497.
Bernardo R, 2002. Breeding for Quantitative Traits in Plants. Stemma press. Woodbury, MN, USA.   369 pp.
Bhandari HR, Bhanu AN, Srivastava K, Singh MN, and Shreya HA, 2017. Assessment of genetic diversity in crop plants-an overview. Advances in Plants and Agriculture Research 7(3): 279-286.
Carlen C, 2016. Breeding and cultivation of medicinal plants. In: Bagetta G, Cosentino M, Corasaniti MT, and Sakurada S (eds). Herbal Medicines: Development and Validation of Plant-derived Medicines for Human Health. Pp. 79-91. CRC Press.
Carpentier S, Costa C, Schurr U, Loreto F, and Menesatti P, 2019. Plant phenotyping research trends, a science mapping approach. Frontiers in Plant Science 9: 1933.
Causse M, Damidaux R, and Rousselle P, 2007. Traditional and enhanced breeding for quality traits in tomato. In: Razdan MK (ed.) Genetic Improvement of Solanaceous Crops: Volume 2 Tomato. Pp. 153-192. Science Publ., NH, USA.
Chesnokov YV, Kosolapov VM, and Savchenko IV, 2020. Morphological genetic markers in plants. Russian Journal of Genetics 56(12): 1406-1415.
Choudhary S, Kaurav H, and Chaudhary G, 2021. Kasani beej (Cichorium intybus): Ayurvedic view, folk view, phytochemistry and modern therapeutic uses. International Journal for Research in Applied Sciences and Biotechnology 8(2): 114-125.
Cirak C, Radusiene J, Karabuk B, Janulis V, and Ivanauskas L, 2007. Variation of bioactive compounds in Hypericum perforatum growing in Turkey during its phenological cycle. Journal of Integrative Plant Biology 49(5): 615-620.
Costa MF, Durço AO, Rabelo TK, Barreto RS, and Guimaraes AG, 2019. Effects of carvacrol, thymol and essential oils containing such monoterpenes on wound healing: a systematic review. Journal of Pharmacy and Pharmacology 71(2): 141-155.
Darkwa K, Agre P, Olasanmi B, Iseki K, Matsumoto R, Powell A, and Asfaw A, 2020. Comparative assessment of genetic diversity matrices and clustering methods in white Guinea yam (Dioscorea rotundata) based on morphological and molecular markers. Scientific Reports 10: 13191.
Fasoula DA, Ioannides, IM, and Omirou, M, 2020. Phenotyping and plant breeding: overcoming the barriers. Frontiers in Plant Science 10: 1713.
Figueiredo AC, Barroso JG, Pedro LG, and Scheffer JJ, 2008. Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour and Fragrance Journal 23(4): 213-226.
Formisano C, Rigano D, and Senatore F, 2011. Chemical constituents and biological activities of Nepeta species. Chemistry and Biodiversity 8(10): 1783-1818.
Fu YB, 2015. Understanding crop genetic diversity under modern plant breeding. Theoretical and Applied Genetics 128(11): 2131-2142.
Ghani A, Amini S, Mohtashami S, and Neamati SH, 2022. Physicochemical and biochemical variations of jujube (Ziziphus jujuba Mill.) populations: a comparison of Iranian and imported types. Industrial Crops and Products 183: 1-11.
Goudarzi T, Saharkhiz MJ, and Rowshan V, 2015. Ontogenetic variation of essential oil content and constituents in tansy (Tanacetum vulgare L.). Journal of Applied Research on Medicinal and Aromatic Plants 2(2): 48-53.
Haghi G, Arshi R, Ghazian F, and Hosseini H, 2012. Chemical composition of essential oil of aerial parts of Cichorium intybus L. from Iran. Journal of Essential Oil Bearing Plants 15(2): 213-216.
Heywood VH, 2002. The conservation of genetic and chemical diversity in medicinal and aromatic plants. In: ┼×ener B (ed.) Biodiversity. Springer, Boston, MA, USA, pp. 13-22.
Imam, KMSU, Xie Y, Liu Y, Wang F, and Xin F, 2019. Cytotoxicity of Cichorium intybus L. metabolites. Oncology Reports 42(6): 2196-2212.
Janda K, Gutowska I, Geszke-Moritz M, and Jakubczyk K, 2021. The common chicory (Cichorium intybus L.) as a source of extracts with health-promoting properties: a review. Molecules 26(6): 1-4.
Karimi A, Krahmer A, Herwig N, Schulz H, Hadian J, and Meiners T, 2020. Variation of secondary metabolite profile of Zataria multiflora Boiss. populations linked to geographic, climatic, and edaphic factors. Frontiers in Plant Science 11: 969.
Julsing MK, Quax WJ, and Kayser O. 2006. The Engineering of medicinal plants: Prospects and limitations of medicinal plant biotechnology. In: Kayser et al. (eds). Medicinal Plant Biotechnology: From Basic Research to Industrial Applications. WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany, pp. 1-8.
Khan MF, Nasr FA, Noman OM, Alyhya NA, Ali I, Saoud M, and Hussain H, 2020. Cichorins D–F: three new compounds from Cichorium intybus and their biological effects. Molecules 25(18): 1-11.
Li Y, Ren M, Wang J, Ma R, Chen H, Xie Q, Li H, Li J, and Wang J, 2021. Progress in borneol intervention for ischemic stroke: a systematic review. Frontiers in Pharmacology 4(12): 606682.
 McLafferty FW, Zhang MY, Stauffer DB, and Loh SY, 1998. Comparison of algorithms and databases for matching unknown mass spectra. In: McLafferty FW and Stauffer DB (eds.). Wiley/NBS Registry of Mass Spectral Data Vol. 1. Wiley, New York, USA, pp. 31-39.
Mozaffarian V, 2012. Identification of medicinal and aromatic plants of Iran. Farhang Moaser Publishers, Tehran. 1444 pp. (In Persian).
Nasimi Doost Azgomi R, Karimi A, Tutunchi H, and Moini Jazani A, 2021. A comprehensive mechanistic and therapeutic insight into the effect of chicory (Cichorium intybus) supplementation in diabetes mellitus: A systematic review of literature. International Journal of Clinical Practice 75(12): 1-14.
Neel MC and Ellstrand NC, 2003. Conservation of genetic diversity in the endangered plant Eriogonum ovalifolium var. vineum (Polygonaceae). Conservation Genetics 4(3): 337-352.
Nwafor IC, Shale K, and Achilonu MC, 2017. Chemical composition and nutritive benefits of chicory (Cichorium intybus) as an ideal complementary and/or alternative livestock feed supplement. Scientific World Journal 2017: 1-12.
Panguluri SK and Kumar AA, 2016. Phenotyping for Plant Breeding. Springer-Verlag, NY, USA. 222 pp.
Pena-Espinoza M, Valente AH, Thamsborg SM, Simonsen HT, Boas U, Enemark HL, and Williams AR, 2018. Antiparasitic activity of chicory (Cichorium intybus) and its natural bioactive compounds in livestock: a review. Parasites and Vectors 11(1): 1-14.
Perovic J, Saponjac VT, Kojic J, Krulj J, Moreno DA, Garcia-Viguera C, and Ilic N, 2021. Chicory (Cichorium intybus L.) as a food ingredient–nutritional composition, bioactivity, safety, and health claims: a review. Food chemistry 336: 1-12.
Polatoglu K, Demirci B, Demirci F, Goren N, and Baser KC, 2012. Biological activity and essential oil composition of two new Tanacetumc hiliophyllum (Fisch. and Mey.) Schultz Bip. var. Chiliophyllum chemotypes from Turkey. Industrial Crops Production 39: 97-105.
Puhlmann ML and De Vos WM, 2020. Back to the roots: revisiting the use of the fiber-rich Cichorium intybus L. taproots. Advances in Nutrition 11(4): 878-889.
Rahimmalek M, Fadaei Heidari E, Ehtemam MH, and Mohammadi, S, 2017. Essential oil variation in Iranian ajowan (Trachyspermum ammi (L.) Sprague) populations collected from different geographical regions in relation to climatic factors. Industrial Crops and Products 95: 591-598.
Razavi S and Enferadi M, 2022. Essential oil composition and metabolites changes of Artemisia melanolepis Boiss at different phenological stages. Journal of Plant Physiology and Breeding 12(1): 39-49.
Roberfroid MB, 2005. Introducing inulin-type fructans. British Journal of Nutrition 93(1): 13-25.
Rustaiyan A, Masoudi S, Ezatpour L, Danaii E, Taherkhani M, and Aghajani Z, 2011a. Composition of the essential oils of Anthemis Hyalina DC., Achillea Nobilis L. and Cichorium intybus L. three asteraceae herbs growing wild in Iran. Journal of Essential Oil Bearing Plants 14(4): 472-480.
Rustaiyan A, Masoudi S, Eghbali H, and Salehi HR, 2011b. Phenological variation on essential oil composition of Stachys inflata Benth. from Iran. Journal of Essential Oil Bearing Plants 14(6): 679-683.
Saeidi K, Moosavi M, Lorigooini Z, and Maggi F, 2018. Chemical characterization of the essential oil compositions and antioxidant activity from Iranian populations of Achillea wilhelmsii K. Koch. Industrial Crops and Products 112: 274-280.
Saengkanuk A, Nuchadomrong S, Jogloy S, Patanothai A, and Srijaranai S, 2011. A simplified spectrophotometric method for the determination of inulin in Jerusalem artichoke (Helianthus tuberosus L.) tubers. European Food Research and Technology 233(4): 609-616.
Satyal P, Murray BL, McFeeters RL, and Setzer WN, 2016. Essential oil characterization of Thymus vulgaris from various geographical locations. Foods 5(4): 1-13.
Steegmans M, Iliaens S, and Hoebregs H, 2004. Enzymatic, spectrophotometric determination of glucose, fructose, sucrose, and inulin/oligofructose in foods. Journal of AOAC International 87(5): 1200-1207.
Swarup S, Cargill EJ, Crosby K, Flagel L, Kniskern J, and Glenn KC, 2021. Genetic diversity is indispensable for plant breeding to improve crops. Crop Science 61(2): 839-852.
 Torras J, Grau MD, Lopez JF, and de las Heras FC, 2007. Analysis of essential oils from chemotypes of Thymus vulgaris in Catalonia. Journal of the Science of Food and Agriculture, 87(12): 2327-2333.
Wang W, Xu J, Fang H, Li Z, and Li M, 2020. Advances and challenges in medicinal plant breeding. Plant Science 298: 110573.
Zlatic NM and Stankovic MS, 2017. Variability of secondary metabolites of the species Cichorium intybus L. from different habitats. Plants 6(3): 1-9.
Zuccarini P, 2009. Camphor: risks and benefits of a widely used natural product. Journal of Applied Sciences and Environmental Management 13(2): 69-74.