Effect of salinity stress on the root proteome pattern of spring bread wheat

Document Type : Research Paper


1 Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran

2 Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran; Center of Excellence for Cereal Molecular Breeding, University of Tabriz, Tabriz, Iran

3 ِDepartment of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran; Center of Excellence for Cereal Molecular Breeding, University of Tabriz, Tabriz, Iran


In this experiment, the effect of salt stress on the root proteome pattern of Arg (tolerant to salinity) and Moghan3 (sensitive to salinity) cultivars of wheat was investigated by two-dimensional polyacrylamide gel electrophoresis. Salinity was applied at two levels of 0 and 150 mM in the third-leaf stage for three weeks under greenhouse conditions. The proteome analysis of the roots revealed 120 reproducible protein spots, among which 15 spots showed significant changes in expression. In Moghan3, four protein spots with a significant reduction in expression were identified. In the tolerant cultivar of Arg, 11 protein spots showed significant changes in expression, among which five protein spots had an increased expression and six protein spots had a decreased expression. These proteins were classified based on their function into several groups such as the proteins involved in metabolism and energy, reactive oxygen species scavenging and detoxification, the proteins associated with cell walls, and the proteins involved in folding and degradation. Probably, the tolerance to salt stress in the Arg cultivar was related to the increased expression of pyruvate dehydrogenase, phosphoglycerate mutase, catalase, and malate dehydrogenase proteins. While in the sensitive variety of Moghan3, the decrease in the expression of enolase, peroxidase, and glutathione peroxidase proteins under salinity stress conditions probably caused oxidative stress in the plants due to increased production of reactive oxygen species. These findings can be useful for improving the salt tolerance in the breeding programs of wheat.


Article Title [Persian]

تأثیر تنش شوری بر الگوی پروتئوم ریشه گندم نان بهاره

Authors [Persian]

  • سمیه شهبازی 1
  • محمود تورچی 2
  • محمد مقدم 3
  • سعید اهری زاد 1
  • علی بنده حق 1
1 گروه به نژادی و بیوتکنولوژی گیاهی، دانشگاه تبریز، تبریز
2 گروه به‌نژادی و بیوتکنولوژی گیاهی، دانشگاه تبریز، تبریز؛ قطب علمی اصلاح مولکولی غلات، دانشگاه تبریز، تبریز
3 گروه به‌نژادی و بیوتکنولوژی گیاهی، دانشگاه تبریز، تبریز؛ قطب علمی اصلاح مولکولی غلات، دانشگاه تبریز، تبریز
Abstract [Persian]

در این آزمایش اثر تنش شوری بر الگوی پروتئوم ریشه ارقام ارگ (متحمل به شوری) و مغان 3 (حساس به شوری) گندم با الکتروفورز ژل پلی آکریل آمید دو بعدی بررسی شد. شوری در دو سطح صفر و 150 میلی مولار در مرحله سه برگی به مدت سه هفته در شرایط گلخانه ­ای اعمال شد. پس از تجزیه پروتئوم ریشه، 120 لکه پروتئینی تکرارپذیر آشکار شد که از میان آن­ ها 15 لکه تغییر بیان معنی­ دار نشان دادند. در مغان3 چهار لکه پروتئینی دارای کاهش بیان معنی ­دار شناسایی شد. در رقم متحمل ارگ، 11 لکه پروتئینی تغییر بیان معنی ­دار نشان دادند که در میان آن­ ها پنج لکه پروتئینی افزایش بیان و 6 لکه پروتئینی کاهش بیان داشتند. این پروتئین­ ها بر اساس عملکرد به چند گروه مانند پروتئین ­های دخیل در متابولیسم و ​​انرژی، مهار و سم زدایی گونه ­های اکسیژن فعال، پروتئین­ های مرتبط با دیواره سلولی و پروتئین­ های دخیل در تاخوردگی و تخریب طبقه بندی شدند. احتمالا تحمل به تنش شوری در رقم ارگ با افزایش بیان پروتئین­ های پیروات دهیدروژناز، فسفوگلیسرات موتاز، کاتالاز و مالات دهیدروژناز ارتباط داشت. درحالی که در رقم جساس مغان3 کاهش بیان پروتئین­ های انولاز، پراکسیداز و گلوتاتیون پراکسیداز در شرایط تنش شوری احتمالا به علت افزایش تولید گونه­ های اکسیژن فعال سبب تنش اکسیداتیو در گیاه شده است. این یافته­ ها می­ تواند برای بهبود تحمل به شوری در برنامه­ های اصلاحی گندم مفید واقع شود.

Keywords [Persian]

  • الکتروفورز دوبعدی
  • تجزیه پروتئوم
  • تنش شوری
  • گونه های اکسیژن فعال
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