Micropropagation of Iranian native oregano (Origanum vulgare L.) using growth regulators

Document Type : Research Paper

Authors

1 Department of Medicinal Plants, Arak University, Arak, Iran

2 Department of Horticultural Science, Islamic Azad University, Saveh Branch, Saveh, Iran

Abstract

Wild oregano (Origanum vulgare L.) belonging to the Labiate family is one of the most important medicinal plants in Iran and the world. It is widely used in the medicinal, food, and health industries. The present study aimed to investigate the in vitro culture of Iranian native oregano. Two experiments were designed to investigate the proliferation and rooting of this species. In the proliferation stage, benzyl adenine (BA) was used at four levels (0, 0.5, 1, and 1.5 mg/l) along with 0.25 mg/l indole butyric acid (IBA). For the rooting stage, IBA at four levels (0, 0.5, 1, and 1.5 mg/l) in combination with 0.5 mg/l naphthalene acetic acid (NAA) was used. The results showed that BA caused a significant effect on the leaf number, the number of shoots, the shoot length, chlorophyll index, and the leaf dry weight. The highest number of leaves and shoots and the highest value of chlorophyll index were seen in the medium containing 1.5 mg/l. The longest shoot and the highest leaf dry weight were related to 1 mg/l BA. In addition, the results related to the rooting stage showed that the root length, root diameter, and total dry weight of plantlets were significantly affected by IBA, while the number of roots was not influenced by IBA. The highest root diameter and total dry weight of plantlets were obtained in the medium containing 0.5 mg/l IBA, and the maximum root length was obtained in the MS medium without IBA.

Keywords

Article Title [Persian]

ریزازدیادی مرزنجوش (Origanum vulgare L.) بومی ایران با استفاده از تنظیم کننده‌های رشد

Authors [Persian]

  • محمّدرضا احمدی 1
  • رضا شاه حسینی 1
  • لیلا حکیمی 2
1 گروه گیاهان دارویی، دانشگاه اراک، اراک
2 گروه مهندسی باغبانی، دانشگاه آزاد اسلامی، واحد ساوه، ساوه
Abstract [Persian]

مرزنجوش وحشی (Origanum vulgare L.)، متعلق به خانواده Labiate یکی از مهمترین گیاهان دارویی ایران و جهان است  که به طور گسترده در صنایع دارویی، غذایی و بهداشتی استفاده می­ شود. پژوهش حاضر با هدف بررسی کشت درون شیشه­ ای مرزنجوش بومی ایران انجام شد. دو آزمایش برای بررسی تکثیر و ریشه زایی این گونه گیاهی طراحی شد. در مرحله تکثیر، بنزیل آدنین (BA) در چهار سطح (0، 0/5، 1 و 1/5 میلی گرم در لیتر) همراه با 0/25 میلی گرم در لیتر ایندول بوتیریک اسید (IBA) استفاده شد. برای مرحله ریشه زایی از IBA در چهار سطح (0، 0/5، 1 و 1/5 میلی گرم در لیتر) همراه با 0/5 میلی گرم در لیتر نفتالین استیک اسید (NAA)  استفاده به عمل آمد. نتایج نشان داد که BA بر تعداد برگ، تعداد شاخساره، طول شاخساره، شاخص کلروفیل و وزن خشک برگ تأثیر معنی‌داری دارد. بیشترین تعداد برگ و شاخساره و بیشترین مقدار شاخص کلروفیل در محیط کشت حاوی 1/5 میلی گرم در لیتر مشاهده شد. بیشترین طول شاخساره و وزن خشک برگ در مقایسه با سایر غلظت­ ها مربوط به 1 میلی گرم در لیتر BA بود. علاوه بر این، نتایج مربوط به مرحله ریشه‌زایی نشان داد که طول ریشه، قطر ریشه و وزن خشک کل گیاهچه‌ها به‌طور معنی‌داری تحت تأثیر IBA قرار گرفتند، در حالی که تعداد ریشه تحت تأثیر غلظت IBA قرار نگرفت. بیشترین قطر ریشه و وزن خشک کل گیاهچه­ ها در محیط کشت حاوی 0/5 میلی گرم در لیتر IBA و حداکثر طول ریشه در محیط MS بدون IBA به دست آمد.

Keywords [Persian]

  • ایندول بوتیریک اسید
  • بنزیل آدنین
  • تکثیر
  • ریزازدیادی
  • ریشه زایی
  • مرزنجوش

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Journal of Plant Physiology and Breeding
Volume 12, Issue 2
December 2022
Pages 105-116

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