Micropropagation of Iranian native oregano (Origanum vulgare L.) using growth regulators

Document Type : Research Paper


1 Department of Medicinal Plants, Arak University, Arak, Iran

2 Department of Horticultural Science, Islamic Azad University, Saveh Branch, Saveh, Iran


Wild oregano (Origanum vulgare L.) belonging to the Labiate family is one of the most important medicinal plants in Iran and the world. It is widely used in the medicinal, food, and health industries. The present study aimed to investigate the in vitro culture of Iranian native oregano. Two experiments were designed to investigate the proliferation and rooting of this species. In the proliferation stage, benzyl adenine (BA) was used at four levels (0, 0.5, 1, and 1.5 mg/l) along with 0.25 mg/l indole butyric acid (IBA). For the rooting stage, IBA at four levels (0, 0.5, 1, and 1.5 mg/l) in combination with 0.5 mg/l naphthalene acetic acid (NAA) was used. The results showed that BA caused a significant effect on the leaf number, the number of shoots, the shoot length, chlorophyll index, and the leaf dry weight. The highest number of leaves and shoots and the highest value of chlorophyll index were seen in the medium containing 1.5 mg/l. The longest shoot and the highest leaf dry weight were related to 1 mg/l BA. In addition, the results related to the rooting stage showed that the root length, root diameter, and total dry weight of plantlets were significantly affected by IBA, while the number of roots was not influenced by IBA. The highest root diameter and total dry weight of plantlets were obtained in the medium containing 0.5 mg/l IBA, and the maximum root length was obtained in the MS medium without IBA.


Abdallah SAS, Yakoup MYA, and Abdalla MYH, 2017. Micropropagation of oregano (Origanum syriacum L.) through tissue culture technique. Journal of Plant Production 8(5): 635-639.
Abrahamyan A, Barsevskis A, Crockett S, and Melikyan A, 2014. Distribution modeling and population ecology of wild Melissa officinalis L. (Lamiaceae) and Origanum vulgare L. (Lamiaceae) in Armenia. Journal of Life Sciences 8(8): 690-698.
Acemi A, Ozen F, and Kıran R, 2013. In vitro propagation of Amsonia orientalis Decne. from nodal segments of adult plants. Propagation of Ornamental Plants 13(1): 25-32.
Ainsley PJ, Collins GG, and Sedgley M, 2001. In vitro rooting of almond (Prunus dulcis mill.). In Vitro Cellular and Developmental Biology, Plant 37: 778-785.
Asghari F, Hassani A, Hosseini B, and Farokhi J, 2013. Evaluation of the effects of genotype type and different concentrations of benzyl adenine on direct regeneration of basil shoots (Ocimum basilicum) under in vitro conditions. Journal of Horticultural Science 26(4): 434-439.
Aghaye RNM, Yadollahi A, Moeini A, and Sepahvand S, 2013. In vitro culture of Gisela 6 semi-dwarf rootstock. Journal of Environmental Sciences 7: 57-64.
Darroudi H, Akbarinia M, Safarnejad A, Hosseini SM. and Hajian Shahri M, 2015. Micropropagation of Ribes khorasanicum species by tissue culture. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research 23(1): 65-76 (In Persian with English abstract).
Dobranszki J and Teixeira da Silva JA, 2010. Micropropagation of apple- A review. Biotechnology Advances 28: 462-488.
El Beyrouthy M, Elian G, bou Jaoudeh CA, and Chalak L, 2013. In vitro propagation of Origanum syriacum and Origanum ehrenbergii. Acta Horticulturae 1083: 169-172.
George EF, 1996. Plant propagation by tissue culture. Part 2: In practice. Second ed. Exegetics Limited, British Library, UK.
Islam MS, Hoque MA, Sumi SA, Jony M, Kamrunnahar, SAR, and Shamsuzzaman M, 2020. Effects of foliar application of indol butyric acid (IBA), gibberellic acid (GA3) and zinc (Zn) on yield and quality of tomato. International Journal of Plant and Soil Science 32(1): 1-9.
Jalili Marandi R, Naseri L, Mohseni Azar M, Hajitaghilu R, and Marhamati MR, 2011. Investigation on interaction effect of benzyl adenine and chitosan on in vitro proliferation of strawberry (Fragaria × Ananassa cv. Selva). Biotechnology in Agriculture 10(1): 27-36.
Karfarma S and Shirzadi MH, 2014. Comparison of the effects of growth regulators of indole butyric acid, naphthalene acetic acid and indole acetic acid by priming and foliar application on tomato seedlings. First National Conference on Medicinal Plants, Traditional Medicine, and Organic Agriculture. November 29, Iran (in Persian).
Kizil S and Khawar K, 2017. Efficient mass propagation of Origanum acutidens (Hand.-Mazz.) Ietswaart under in vitro conditions. Bangladesh Journal of Botany 46(2): 667-673.
Meena M, Meena R, and Patni V, 2010. High frequency plant regeneration from shoot tip explants of Citrullus colocynthis an important medicinal herb. African Journal of Biotechnology 9(31): 5037-5041.
Meftahizade H, Moradkhani H, Naseri B, Lotfi M, and Naseri A, 2010. Improved in vitro culture and micropropagation of different Melissa officinalis L. genotypes. Journal of Medicinal Plants Research 4: 240-246.
Molassiotis AN, Dimassi K, Therios I, and Diamantidis G, 2003. Fe-EDDHA promotes rooting of rootstock GF-677 (Prunus amygdalus × P. persica) explants in vitro. Biologia Plantarum 47(1): 141-144.
Moradi M, Hassani A, Sefidkon F, and Maroofi H, 2021. Qualitative and quantitative changes in the essential oil of origanum vulgare ssp. gracile as affected by different harvesting times. Journal of Agricultural Science and Technology 23(1): 179-186.
Murashige T and Skoog F, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473-497.
Premi N, Acemi A, and Ozen F, 2021. Cytokinin-like effects of chitosan on in vitro culture of Origanum vulgare L. Italus Hortus 28(1): 100-108.
Roberson D, Cristiane L, Francine L, Henrique K, and Marguerite Q, 2005. Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis. Science Agriculture 62: 406-412.
Saber Hamishegi Z, Tarang AR, Dehpoori AA, and Saber Hamishegi F, 2011. The effect of different auxin levels on Stevia micro propagation in vitro. National Conference on Advances in Agriculture, November 16, Iran (In Persian).
Sevindik B, Izgu T, Şimsek O, Tutuncu M, Curuk P, Yılmaz O, Kaynak G, Aka Kacar Y, Teixeira da Silva JA, and Yalcın Mendi Y, 2017. In vitro culture of Turkish Origanum sipyleum L. American Journal of Plant Biology 2(5): 32-36.
Shakouri MJ, Mohammadi J, Shahmohammadi S, and Kapourchal SA, 2012. Assessing the effect of different levels of NAA and time on Dracaena sanderiana (lucky bamboo). Indian Journal of Science and Technology 5(1): 1924-1927.
Spada P and Perrino P, 1997. Conservation of oregano species in national and international collections: An assessment. Proceedings of the IPGRI International Workshop on Oregano, May 8-12, CIHEAM, Valenzano (Bari), Italy.
Thorpe T, Stasolla C, Yeung EC, de Klerk GJ, Roberts A, and George EF, 2008. The components of plant tissue culture media II: Organic additions, osmotic and pH effects, and support systems. In: George EF, Hall MA, and De Klerk GJ (eds.). Plant Propagation by Tissue Culture. Springer 1: 115-173.
Torabi Giglu M, Masiha S, Majidi E, Khosroshahli M, and Valizadeh M, 2001. Determining the most suitable growth regulator in in vitro culture of end of clove shoot (Dianthus caryophyllus L.). Agricultural Knowledge 11(2): 41-50.
Torres KC, 1989. Tissue culture media-composition and preparation. In: Tissue Culture Techniques for Horticulture Crops, New York, pp. 26-51.
Turker AH and Hatipoglu R, 2018. Micropropagation of Bible Hyssop (Origanum syriacum L. var. bevanii (Holmes) Ietswaart). Turkish Journal of Forestry Research 5(2): 97-111.