Effect of nitrogen-fixing bacteria and mycorrhiza on biochemical properties and absorption of essential elements in green pea (Pisum sativum L.) under water deficit stress

Document Type : Research Paper

Authors

Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabil, Iran

Abstract

Drought stress is a critical abiotic stress that has a negative effect on plant productivity. Plant growth-promoting rhizobacteria (PGPR) such as Azotobacter and Azospirillium positively affect plant physiology, especially under drought stress. The recent study aimed to examine the effects of Mycorrhiza fungi and PGPR on the activity of antioxidant enzymes and the amount of nutrient absorption under water deficit conditions. A factorial experiment was performed based on a randomized complete block design with three replications. Factors were irrigation (regular irrigation, water deficit at the grain filling stage, water deficit at the flowering stage, no irrigation) and Mycorrhiza fungi and PGPR (Azotobacte rcoroccum, Azospirillum lipoferum, Mycorrhiza arbuscularas, Azotobacter coroccum + Azospirillum lipoferum, Azotobacte rcoroccum + Mycorrhiza arbuscular, Azospirillum lipoferum + Maycorrhiza arbuscular, Azotobacte rcoroccum + Azospirillum lipoferum + Mycorrhiza arbuscular, no inoculation). Drought stress decreased chlorophyll content and absorption of elements and increased proline, catalase, and peroxidase activity. Also, biofertilizers reduced the amount of proline, catalase, and peroxidase. They increased the amount of chlorophyll content and absorption of elements.

Keywords

References

Abo-Baker AA and Mostafa GG, 2011. Effect of bio-and chemical fertilizers on growth, sepals yield and chemical composition of Hibiscus abdariffa at new reclaimed soil of south valley area. Asian Journal of Crop Science 3:16-25.
Agarwal S and Pandey V, 2004. Antioxidant enzyme responses to NaCl stress in Cassia angustifolia. Biologia Plantarum 48: 555-560.
Allen M, Moore JTS, and Christensen M, 1982. Phytohormone changes in Boutelou agracilis infected by vesicular-arbuscular mycorrhizae: II. Altered levels of gibberellin-like substances and abscisic acid in the host plant. Canadian Journal of Botany 60: 468-471.
Al-Naggar AMM, Abd El-Salam RM, Badran AEE, and El-Moghazi MA, 2017. Drought tolerance of five quinoa (Chenopodium quinoa Willd.) genotypes and its association with other traits under moderate and severe drought stress. Asian Journal of Advances in Agricultural Research 3: 2456-2468.
Anonymous, 1980. Official methods of analysis. 13th ed. The Association of Official Analytical Chemists. Washington DC, USA.
Ansari FA and Ahmad I, 2019. Alleviating drought stress of crops through PGPR: mechanism and application. In: Singh D, Gupta V, and Prabha R (eds). Microbial Interventions in Agriculture and Environment. Springer, Singapore.
Atouei MT, Pourbabaee AA, and Shorafa M, 2019. Alleviation of salinity stress on some growth parameters of wheat by exopolysaccharide-producing bacteria. Iran. Journal of Science and Technology 43: 2725-2733.
Barnawal D, Bharti N, Pandey SS, Pandey A, Chanotiya CS, and Kalra A, 2017. Plant growth-promoting rhizobacteria enhance wheat salt and drought stress tolerance by altering endogenous phytohormone levels and TaCTR1/TaDREB2 expression. Physiologia Plantarum 161: 502–514.
Basra SMA, Iqbal S, and Afzal I, 2014. Evaluating the response of nitrogen application on growth, development, and yield of quinoa genotypes, International Journal of Agriculture and Biology 16: 886-892.
Behl RK, Narula N, Vasudeva M, Sato A, Shinano T, and Osaki M, 2006. Harnessing wheat genotype xAzotobacter strain interactions for sustainable wheat production in semi-arid tropics. Tropics 15: 123-133.
Chance B and Maehly SK, 1955. Assay of catalase and peroxidase. Methods in Enzymology 2: 764-775.
Das K and Roychoudhury A, 2014. Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Frontiers in Environmental Science 2: 53-66.
Enebe MC and Babalola OO, 2018. The influence of plant growth-promoting rhizobacteria in plant tolerance to abiotic stress: a survival strategy. Applied Microbiology and Biotechnology 102: 7821-7835.
Fang Y and Xiong L, 2015. General mechanisms of drought response and their application in drought resistance improvement in plants. Cellular and Molecular Life Sciences 72: 673-689.
Farooq M, Hussain M, and Siddique KHM, 2014. Drought stress in wheat during flowering and grain-filling periods. Critical Reviews in Plant Sciences 33: 331-349.
Forni C, Duca D, and Glick BR, 2017. Mechanisms of plant response to salt and drought stress and their alteration by rhizobacteria. Plant and Soil 410: 335-356.
Fouad MO, Essahibi A, Benhiba A, and Qaddoury A, 2014. Effectiveness of arbuscular mycorrhizal fungi in the protection of olive plants against oxidative stress induced by drought. Spanish Journal of Agricultural Research 12:763-771.
Gilik BR, Penrose D, and Wenbo M, 2001. Bacterial promotion of plant growth. Biotechnology Advances 19: 135-138.
Hinojosa L, González J, Barrios-Masias F, Fuentes F, and Murphy K, 2018. Quinoa abiotic stress responses: a review. Plants 7: 106-138.
Jackson ML, 1973. Soil Chemical Analysis. Prentice-Hall, New Delhi.
Jones HG, 1980. Interaction and integration of adaptive response to water stress. Royal Science Society of London 273: 193-205.
Kamara AY, Ekeleme F, Chikoye D, and Omoigui LO, 2009. Planting date and cultivar effects on grain yield in dryland corn production. Agronomy Journal 101: 91-98.
Kang SM, Radhakrishnam R, Khan AL, Kim MJ, Park JM, Kim BR, Shin DH, and Lee IJ, 2014. Gibberellin secreting rhizobacterium, Pseudomonas putida H-2-3 modulates the hormonal and stress physiology of soybean to improve the plant growth under saline and drought conditions. Plant Physiology and Biochemistry 84: 115-124.
Marschner H, 1995. Mineral Nutrition of Higher plants. Academic Press, USA, 889pp.
Meher MP, Ashok RK, and Manohar RD, 2018. Effect of PEG-6000 imposed drought stress on RNA content, relative water content (RWC), and chlorophyll content in peanut leaves and roots. Saudi Journal of Biological Science 25:285-289.
Mir RR, Zaman-Allah M, Sreenivasulu N, Trethowan R, and Varshney RK, 2012. Integrated genomics, physiology, and breeding approaches for improving drought tolerance in crops. Theoretical and Applied Genetics 125: 625-645.
Nemeskeri E, Molnar K, Racz C, Dobos AC, and Helyes L, 2019. Effect of water supply on spectral traits and their relationship with the productivity of sweet corns. Agronomy 9: 63.
Ngumbi E and Kloepper J, 2016. Bacterial-mediated drought tolerance: current and future prospects. Applied Soil Ecology 105: 109-125.
Parray JA, Jan S, Kamili AN, Qadri RA, Egamberdieva D, and Ahmad P, 2016. Current perspectives on plant growth-promoting rhizobacteria. Journal of Plant Growth Regulation 35: 877–902.
Ray P, 2015. Hi-tech horticulture climate change. In: Choudhary ML, Patel VB, Siddiqui MW, and Mahdi SS (eds). Climate Dynamics in Horticultural Science, Principles and Applications. Apple Academic Press, NY, USA, pp. 1-22.
Ruiz-Lozano JM, 2003. Arbuscular­ mycorrhizal symbiosis and alleviation of osmotic stress, new perspectives for molecular studies. Mycorrhiza 13: 309-317.
Sairam RK, Deshmukh PS, and Saxna DC, 2011. Role of antioxidant systems in Anethum genotype tolerance to water stress. Journal of Applied Environmental and Biological Sciences 41: 387-394.
Salehi A, Tasdighi H, and Gholamhoseini M, 2016. Evaluation of proline, chlorophyll, soluble sugar content, and uptake of nutrients in the German chamomile (Matricaria chamomilla L.) under drought stress and organic fertilizer treatments. Asian Pacific Journal of Tropical Biomedicine 6: 886-891.
Schutz M and Fangmeir E, 2001 Growth and yield responses of spring wheat (Triticumaestivum L. cv. Minaret) to elevated CO2 and water limitation. Environmental Pollution 114: 187-194.
Sharma KD and Kuhad MS, 2006. Influence of potassium level and soil moisture regime on biochemical metabolites of Brassica Species. Brassica Journal 8: 71-74.
Shirinbayan S, Khosravi H, and Malakouti MJ, 2019. Alleviation of drought stress in maize (Zea mays) by inoculation with Azotobacter strains isolated from semi-arid regions. Applied Soil Ecology 133:138-145.
Singh M, Khan MMA, and Naeem M, 2016. Effect of nitrogen on growth, nutrient assimilation, essential oil content, yield, and quality attributes in Zingiber officinale Rosch. Journal of the Saudi Society of Agricultural Sciences 15: 171-178.
Sinha AK, 1972. Colorimetric assay of catalase. Analytical Biochemistry 47: 389-394.
Vurukonda SS, Vardharajula S, Shrivastava M, and Sk ZA, 2016. Enhancement of drought stress tolerance in crops by plant growth-promoting rhizobacteria. Microbiological Research 184: 13-24.
Wu Q and Xia R, 2006. Arbuscular mycorrhizal fungi influence growth, osmotic adjustment, and water stress conditions. Journal of Plant Physiology 163: 417-425.
You J and Chan Z, 2015. ROS regulation during abiotic stress responses in crop plants. Frontiers in Plant Science 6: 1092.
Journal of Plant Physiology and Breeding
Volume 12, Issue 2
December 2022
Pages 59-70

Files

Share

How to cite

Statistics