Characterization of some wild Berberis sp. genotypes distributed in the northeast of Iran

Document Type : Research Paper

Authors

1 Former PhD student, Horticultural Sciences Department, Faculty of Plant Productions, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

2 Horticultural Sciences Department, Faculty of Plant Productions, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

3 Department. of Plant Breeding, Faculty of Plant Productions, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

Abstract

The wild barberry shrubs, commonly known as black barberry, are naturally widespread in the north and northeast elevations of Iran. Besides a rich gene bank, these are highly considered concerning food and medicinal purposes. The present research work was undertaken to evaluate the genetic diversity among 15 different barberry genotypes (14 wild and one cultivated genotypes) through morphological, biochemical, and molecular markers. The morphological traits of leaf, thorn, and berry were measured. The biochemical properties of the studied genotypes were also measured at the fruit ripening stage. Furthermore, the genotypes were subjected to simple sequence repeat analysis to ascertain their genetic diversity at the molecular level. The studied accessions were diverse in the case of morphological traits and they were classified into five distinct groups. Moreover, some rare and remarkable morphological characteristics were found in the fruit shape and fruit clusters of some genotypes not reported earlier. Though wide differences were obtained concerning fruit biochemical compounds, the differences didn’t have a clear trend. The accessions were characterized based on microsatellite analysis into eight groups in which the closely related genotypes had relatively higher geographical similarities. Access to the genetic diversity of these genotypes may be considered as the backbone of their future breeding programs and the reported data supported that the northeast of Iran may be assumed as a rich source for the diversity of Berberis germplasm.

Keywords

Article Title [Persian]

ارزیابی خصوصیات برخی از ژنوتیپ های زرشک وحشی (.Berberis sp) در شمال شرق ایران

Authors [Persian]

  • سمیه طالبی 1
  • مهدی علیزاده 2
  • عظیم قاسم نژاد 2
  • سیده ساناز رمضانپور 3
1 دانش‌ آموخته دکتری، گروه باغبانی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان
2 گروه باغبانی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان
3 گروه اصلاح و بیوتکنولوژی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان
Abstract [Persian]

درختچه‌های زرشک وحشی که به طور معمول، به نام «زرشک سیاه» شناخته می‌شوند، به‌طور طبیعی در ارتفاعات شمال و شمال شرق ایران پراکنش دارند. این توده ­ها، علاوه بر یک بانک ژن غنی، از نظر اهداف غذایی و دارویی بسیار مورد توجه می ­باشند. پژوهش حاضر به منظور بررسی تنوع ژنتیکی 15 ژنوتیپ مختلف زرشک (14 ژنوتیپ وحشی و یک ژنوتیپ زراعی) از طریق نشانگرهای مورفولوژیکی، بیوشیمیایی و مولکولی انجام شد. صفات مورفولوژیکی برگ، خار و میوه اندازه­ گیری شد. خواص بیوشیمیایی ژنوتیپ­ های مورد مطالعه نیز در مرحله رسیدن میوه اندازه­ گیری شد. علاوه بر این، ژنوتیپ‌ها با نشانگرهای ریزماهواره تجزیه و تحلیل شدند تا تنوع ژنتیکی آن ­ها در سطح مولکولی نیز مشخص شود. توده­ های مورد مطالعه از نظر صفات مورفولوژیکی متنوع بودند و در پنج گروه مجزا طبقه­بندی شدند. علاوه بر این، برخی از ویژگی‌های مورفولوژیکی کمیاب و قابل توجه در شکل میوه و خوشه‌های میوه در تعدادی از ژنوتیپ‌ها یافت شد که قبلاً گزارش نشده بود. اگرچه تفاوت­ های زیادی در مورد ترکیبات بیوشیمیایی میوه به دست آمد، ولی این تفاوت­ ها روند مشخصی نداشتند. این توده­ ها، بر اساس نشانگرهای ریزماهواره در هشت گروه قرار گرفتند که در آن­ ها ژنوتیپ­ های نزدیک به هم شباهت­ های جغرافیایی نسبتاً بیشتری داشتند. دسترسی به تنوع ژنتیکی این ژنوتیپ ­ها را می­ توان به عنوان ستون فقرات برنامه ­های اصلاحی آتی آن­ ها در نظر گرفت و داده­ های گزارش شده در این پژوهش حاکی از آن است که شمال شرق ایران می تواند یک منبع غنی برای تنوع ژرم پلاسم زرشک در نظر گرفته شود.
 

Keywords [Persian]

  • به‌نژادی
  • تنوع
  • ریزماهواره
  • زرشک
  • نشانگر

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Journal of Plant Physiology and Breeding
Volume 12, Issue 1
May 2022
Pages 135-152

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