Effect of chitosan on morpho-physiological traits and regeneration of Iris pseudacorus plantlets under in vitro conditions

Document Type : Research Paper

Authors

Department of Horticulture, University of Mohaghegh Ardabili, Ardabil, Iran

Abstract

 
Iris pseudacorus is a highly valuable ornamental and medicinal plant. Chitin is one of the most abundant polysaccharides in nature and is widely used in agriculture for seed germination to stimulate plant growth. Chitosan can be used as an antibacterial component and can increase plant resistance to diseases. This study was conducted based on a completely randomized design. Treatments consisted of different concentrations of chitosan (0, 5, 10, 20, 40, 80, and 120 ppm) with five replications. Morphological and physiological traits including leaf number, leaf weight, plantlets height, leaf area, percentage of regeneration, chlorophyll a, b and total, total phenol, and flavonoids were evaluated. Results of the analysis of variance showed that chitosan significantly affected leaf number, regeneration percentage, phenol content, and leaf fresh weight. Flavonoids, chlorophyll a and b, total chlorophyll were also significantly affected. Results also showed that the highest (31.60 mg/g gallic acid) and lowest (15.51 mg/g gallic acid) total phenol content was obtained from 120 ppm chitosan and control samples, respectively. The highest flavonoid content (5.78 mM/g) was obtained by 120 ppm chitosan and the lowest value (3.20 mM/g) was recorded in the control treatment. In general, our investigation showed that chitosan had a positive effect on all measured traits. In most of the measured traits, the best chitosan concentration was 120 ppm.

Keywords

References

Amiri A, Sirousmehr A, and Esmaeilzadeh Bahabadi S, 2015. Effect of foliar application of salicylic acid and chitosan on yield of safflower (Carthamus tinctorius L.). Journal of Plant Research (Iranian Journal of Biology) 28(4): 712-725 (In Persian with English abstract).
Arikat NA, Jawad FM, Karam NS, and Shibli RA, 2004. Micropropagation and accumulation of essential oils in wild sage (Salvia fruticosa Mill). Scientia Horticulturae 100(1-4): 193-202.
Arnon DI, 1949. Copper enzymes in isolate chloroplasts: polyphenoloxidase in Beta vulgaris.  Plant Physiology 24(1): 1-24.
Chibu H and Shibayama H, 2003. Effects of chitosan application on the growth of several crops. In: Uragami T, Kurita K, and Fukamizo T (eds.). Chitin and Chitosan in Life Science. Yamaguchi, Japan, pp. 235-239.
Coskun Y, Ragbet ED, Zeynep O, Nazife Y, and Kubra T, 2015. Chitosan improves plant regeneration in callus culture of a medicinal herb. Melissa officinalis L. Journal of Biotechnology 208: S5-S120. 
Dzung NA and Thang NT, 2002. Effects of oligoglucosamine prepared by enzyme degradation on the growth of soybean. Advances in Chitin Science 5: 463-467.
Dzung NA, Phuong Khanh VT, and Dzung TT, 2011. Research on impact of chitosan oligomers on biophysical characteristics, growth, development and drought resistance of coffee. Carbohydrate Polymer 84: 751-755.
Emami Bistgani Z, Siadat SA, Bakhshandeh A, and Ghasemi Pirbaloti A, 2015. Effects of chemical and organic fertilizers and chitosan on physiological traits and phenolic compound amounts in thyme (Thymus deanensis Celak) in Shahrekord region. Journal of Crop Production Research 7(1): 11-26 (In Persian with English abstract).
Forouzandeh M, Mohkami Z, and Fazelinasab B, 2019. Evaluation of biotic elicitors foliar application on functional changes, physiological and biochemical parameters of fennel (Foeniculum vulgare). Journal of Plant Production Research 25(4): 49-65. (In Persian with English abstract).
Gornik K, Grzesik M, and Romanowska-Duda B, 2008. The effect of chitosan on rooting of grapevine cuttings and on subsequent plant growth under drought and temperature stress. Journal of Fruit and Ornamental Plant Research 16: 333-343.
Guan YJ, Hu J, Wang XJ, and Shao CX, 2009. Seed priming with chitosan improves maize germination and plantlet growth in relation to physiological changes under low temperature stress. Journal of Zhejiang University-Science B 10(6): 427-433.
Hien QN, 2004. Radiation degradation of chitosan and some biological effects. In:  Radiation Processing of Polysaccharides. International Atomic Energy Agency (IAEA), Austria, pp. 67-74.
Jami S, Esmaeilzadeh Bahabadi S, and Modarres M, 2018. Effect of chitosan on micropropagation, secondary metabolites content and antioxidant activity of Salvia leriifolia Benth. Journal of Plant Research (Iranian Journal of Biology) 31(3): 710-725. (In Persian with English abstract).
Khalafi M, Pourbeyrami Hir Y, Chamani E, and Maleki Lajayer H, 2022. The effect of chitosan on regeneration and secondary metabolite production of two species of lily flower. Journal of Plant Research (Iranian Journal of Biology) 35(2): 374-388.
Khan R, Manzoor N, Zia A, Ahmad I, Ullah A, Shah SM, Naeem M, Ali Sh, Habib Khan I, Zia D, and Malik Sh, 2018 .Exogenous application of chitosan and humic acid effects on plant growth and yield of pea (Pisums ativum). International Journal of Biosciences 12(5): 43-50.
Kim KW and Tomas RL, 2007. Antioxidative activity of chitosan with varying molecular weights. Journal of Food Chemistry 101(1): 308-313.
Kowalski B, Terry FJ, Herrera L, and Penalver DA, 2006. Application of soluble chitosan in vitro and in the greenhouse to increase yield and seed quality of potato minitubers. Potato Research 49: 167-176.
Krizk DT, Kramer GF, Upadhyaya A, and Mirecki RM, 1993. UV-B response of cucumber seedlings grown under metal halide and high pressure sodium/deluxe lamps.  Physiologia Plantarum 88(2): 350-358.
Kumar S, Kanwar JK, and Sharma DR, 2006. In vitro propagation of Lilium. Advances in Horticultural Science 20(2): 181-188.
Limpanavech P, Chaiyasuta S, Vongpromek R, Pichyangkura R, Chadchawan S, Lotrakul P, Bunjongra R, Chaidee A, and Bangyeekhun T, 2008. Chitosan effects on floral production, gene expression and anatomical changes in the Dendrobium orchid. Scientia Horticulturae 116(1): 65-72.
Liu J, Tian S, Meng X, and Xu Y, 2007. Effects of chitosan on control of postharvest diseases and physiological responses of tomato fruit. Journal of Postharvest Biology and Technology 44: 300-306.
Ma L, Li Y, Yu C, Wang Y, Li X, Li N, Chen Q, and Bu N, 2012. Alleviation of exogenous oligo chitosan on wheat plantlets growth under salt stress. Protoplasma 249(2): 393-399.
Mahdavi B, Modarres Sanavy SAM, Aghaalikhani M, Sharifi M, and Alavi Asl SA, 2014. Effect of foliar application of chitosan on growth and biochemical characteristics of safflower (Carthamus tinctorius L.) under water deficit stress. Iranian Journal of Field Crops Research 12(2): 229-236 (In Persian with English abstract).
Maleki Lajayer H, Hamidoghli Y, Zakizadeh H, Bigluei MH, and Chamani E. 2018. Evaluation of the possibility of using thyme (Thymus spp.) species in landscape. Iranian Journal of Horticultural Science 49(3): 647-655 (In Persian with English abstract).
Malekpoor F, Salimi A, and Ghasemi Pirbalouti A, 2017. Effect of bio-elicitor of chitosan on physiological and morphological properties in purple basil (Ocimum basilicum L.) under water deficit. Journal of Plant Ecophysiology 8(27): 56-71 (In Persian with English abstract).
Michalak A, 2006. Phenolic compounds and their antioxidant activity in plants growing under heavy stress. Polish Journal of Environmental Studies 1(4): 523-530.
Mondal MMA, Malek MA, Puteh AB, Ismail MR, Ashrafuzzaman M, and Naher L, 2016. Effect of foliar application of chitosan on growth and yield in okra. Australian Journal of Crop Sciences 6(5): 918-921.
Monirul IM, Humayun K, and Mamun ANK, 2018. Das pronabananda studies on yield and yield attributes in tomato and chilli using foliar application of oligo-chitosan. GSC Biological and Pharmaceutical Sciences 3(3): 20-28.
Naderi S, Esmaeilzadeh Bahabadi S, and Fakheri B, 2015. The effect of chitosan on some physiological and biochemistry characterization in basil (Ocimum basilicum). Journal of Plant Process and Function (Iranian Society of Plant Physiology) 4(12): 29-41 (In Persian with English abstract).
Nourafcan H, 2019. Effect of chitosan on physiological and morphological traits of lemon verbena (Lippia citriodora L.) under in vitro and field conditions. Journal of Crop Ecophysiology 13: 73-86 (In Persian with English abstract).
Ouyang S and Langlai X, 2003. Effect of chitosan on nutrient quality and some agronomic characteristics of non-heading Chinese cabbage. Plant Physiology Communication 39(1): 21-24.
Palida S, Rath P, and Supachitra C, 2014. Chitosan increased phenolic compound contents in tea (Camellia sinensis) leaves by pre- and post-treatments. Journal of Chitin and Chitosan Science 2(2): 93-98.
Pourbeyrami Hir Y, Khalafi M, Chamani E, and Maleki Lajayer H, 2021. Effect of chitosan on regeneration and secondary metabolite production of Lilium regale. Journal of Plant Physiology and Breeding 11(2): 147-160
Ritti W, Chourykaew B, Phrombangyuan P, and Thaksin S, 2016. Effect of chitosan on growth and development of in vitro plantlet of Panisea uniflora (Lindl.) Lindl. Songklanakarin Journal of Plant Science 3(4): 8-13.
Salachna PJ and Zawadzińska A, 2014. Effect of chitosan on plant growth, flowering and corms yield of potted freesia. Journal of Ecological Engineering 15(3): 97-102.
Salehi S, Rezayatmand Z, and Ghasemi Pirbalouti A, 2017. The effect of foliar application of chitosan on yield and essential oil of savory (Satureja isophylla L.) under salt stress. Journal of Herbal Drugs 8(2): 101-108.
Slinkard K and Singleton VL, 1977. Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture 28: 49-55.
Sun V, Zhang Y, Wang K, and Qiu X, 2006. NaOH scarification and stratification improve germination of Iris lacteal var. Chinensis seed. HortScience 41:773-774.
Vasconsuelo A and Boland R, 2007. Molecular aspects of the early stages of elicitation of secondary metabolites in plants. Plant Science 172: 861-875.
Villasenor CJ, De Lucas MA, Gomez G.R, and Mena SJ, 2007. A comparative study of five horizontal subsurface flow constructed wetlands using different plant species for domestic wastewater treatment. Environmental Technology 28: 1333-1343.
Yadollahi Dehchecsme P, Bagheri A, Amiri A, and Esmailzade Bahabadi S. 2014. Effect of drought tension and chitosan foliar application on yield and photosynthetic pigments of sunflower (Heliantus annuus L.). Crop Physiology Journal 6(21): 73-83 (In Persian with English abstract).
Yin H,  C Fretté X,  P Christensen L, and  Grevsen K, 2012. Chitosan oligosaccharides promote the content of polyphenols in greek oregano (Origanum vulgare ssp. hirtum). Journal of Agricultural Food Chemistry 60(1): 136-143.
Zhao J and Sakai K, 2003. Multiple signaling pathways mediate fungal elicitor induced beta-thujaplicin biosynthesis in Cupressus lusitanica cell cultures. Journal of Experimental Botany 54(383): 647-656.
Journal of Plant Physiology and Breeding
Volume 12, Issue 2
December 2022
Pages 71-83

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