Effects of selenium nanoparticles and ancymidol on physiological responses of Stevia rebaudiana Bertoni colonized by Piriformospora indica

Document Type : Research Paper


Department of Biology, Faculty of Science, Bu-Ali Sina University, Hamedan, Iran


The South American plant, Stevia rebaudiana Bertoni, is a good source of steviol glycosides, antioxidants, all of the essential amino acids, and other important nutritional compounds. The present study was conducted to investigate the effects of 5 and 10 mg L-1 concentrations of selenium nanoparticles (SeNPs) and 50 mg L-1ancymidol (ANC) on physiological and biochemical characteristics of S. rebaudiana colonized by the root endophytic fungus Piriformospora indica at vegetative and initial flowering stages. Results indicated that ANC decreased root colonization rate and antioxidant enzyme activities, but increased the content of H2O2 (vegetative stage), malondialdehyde (initial flowering stage), and phosphorus uptake (initial flowering stage( significantly and had no effect on total carbohydrate content. The adverse effects of ANC reduced after P. indica colonization and somewhat with SeNPs application. Our results suggest that the P. indica colonization and SeNPs application can alter the equilibrium between the production of free radicals and enzymatic defence reactions in S. rebaudiana by enhancing the scavenging capacity of free radicals and by decreasing membrane lipid peroxidation during both vegetative and initial flowering stages. Moreover, the effects of ANC, SeNPs, and P. indica on the measured characteristics at the vegetative stage were higher than those observed at the initial flowering stage.


Main Subjects

Article Title [فارسی]

اثرات کاربرد نانوذرات سلنیوم و آنسیمیدول بر پاسخ های فیزیولوژیکی گیاه Stevia rebaudiana Bertoni تلقیح شده با قارچ Piriformospora indica

Authors [فارسی]

  • رویا کرمیان
  • معصومه احمدی خویی
گروه زیست شناسی، دانشکده علوم، دانشگاه بوعلی سینا، همدان
Abstract [فارسی]

گیاه استویا بومی آمریکای جنوبی و منبع خوبی از استویول گلیکوزیدها، آنتی­اکسیدان­ ها، اسیدهای آمینه ضروری و سایر ترکیبات مغذی مهم است. در مطالعه حاضر اثر 5 و 10 میلی­ گرم در لیتر نانوذرات سلنیوم (SeNPs) و 50 میلی­گرم در لیتر آنسیمیدول (ANC) در دو مرحله رویشی و آغاز گلدهی، بر پویژگی­ های بیوشیمیایی و فیزیولوژیکی گیاه Stevia rebaudiana، تلقیح شده با قارچ اندوفیت Piriformospora indica مورد بررسی قرار گرفت. نتایج نشان داد که ANC، میزان کلونیزاسیون ریشه و فعالیت آنزیم ­های آنتی­اکسیدانی را کاهش داد، لیکن محتوای پراکسید هیدروژن (مرحله رویشی)، مالون دی آلدئید (مرحله آغاز گلدهی) و جذب فسفات (مرحله آغاز گلدهی) را افزایش داد و تاثیری بر محتوای کربوهیدرات­ های کل نداشت. اثرات منفی ANC پس از کلونیزاسیون ریشه با قارچ و تا حدودی با کاربرد  SeNPs تقلیل یافت. بر اساس نتایج این پژوهش، کلونیزاسیون ریشه با P. indica و کاربرد SeNPs قادر است تعادل میان تولید رادیکال­ های آزاد و واکنش­ های دفاع آنزیمی در گیاه استویا را با افزایش پتانسیل مهار رادیکال آزاد و کاهش پراکسیداسیون لیپیدی غشاء در هر دو مرحله رویشی و آغاز گلدهی تغییر دهد. علاوه بر این، اثرات تیمارهای SeNPs، ANC و تلقیح P. indica در مرحله رویشی بیشتر از مرحله آغاز گلدهی بود.

Keywords [فارسی]

  • آنزیم های آنتی اکسیدانی
  • استویا
  • استویول گلیکوزیدها
  • بازدارنده رشد
  • قارچ اندوفیت
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