Impact of cadmium stress on growth and physiological responses of fenugreek (Trigonella foenum-graecum L.)

Document Type : Research Paper


1 Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran

2 Department of Biology, Faculty of Science, Bu-Ali Sina University, Hamedan, Iran.


Pollution of the environment by toxic metals creates stress conditions that generally negatively affect plant growth and development. Cadmium is a highly toxic metal that enters the environment mainly through industrial activities and affects crops and other agricultural plants. In the current investigation, the influence of different concentrations of CdCl2 on Cd uptake, growth parameters, and antioxidant responses of Trigonella foenum-graecum L. were investigated. Results showed that H2O2 and malondialdehyde contents were increased by Cd treatments compared to the control plants. Cd stress differently altered the activity of antioxidant enzymes such as catalase, superoxide dismutase, peroxidase, and polyphenol oxidase. The content of antioxidant compounds such as phenols, flavonoids, anthocyanins, soluble proteins, and proline was also increased by the Cd treatment. In addition, our results demonstrated the decrease in seed germination percentage, growth parameters, and chlorophyll and carotenoid contents that may be considered circumstantial evidence for the toxicity of cadmium.


Article Title [فارسی]

اثرات تنش کادمیوم بر پاسخ های رشدی و فیزیولوژیکی گیاه شنبلیله (.Trigonella foenum-graecum L)

Authors [فارسی]

  • امیرعلی کمالوند 1
  • سیاوش حسینی سرقین 1
  • رویا کرمیان 2
1 گروه زیست شناسی، دانشکده علوم، دانشگاه ارومیه، ارومیه
2 گروه زیست شناسی، دانشکده علوم پایه، دانشگاه بوعلی سینا، همدان.
Abstract [فارسی]

آلودگی محیط زیست با فلزات سمی شرایط تنش­ باری را به وجود می ­آورد که اغلب رشد و نمو گیاه را به شکل منفی تحت تاثیر قرار می­ دهد. کادمیوم فلزی بسیار سمی است که اساسا از طریق فعالیت ­های صنعتی وارد محیط زیست می­ شود و بر رشد گیاهان زراعی و سایر گیاهان تاثیر می­ گذارد. در پژوهش حاضر اثر غلظت­ های مختلف CdCl2 بر جذب کادمیوم، پارامترهای رشدی و پاسخ­ های آنتی اکسیدانی گیاه شنبلیله مورد مطالعه قرار گرفت. نتایج نشان داد که محتوای مالون­ دی ­آلدئید و پراکسید هیدروژن در گیاهان تیمار شده با کادمیوم در مقایسه با گیاهان شاهد افزایش یافت. تنش کادمیوم به طرز متفاوتی فعالیت آنزیم ­های آنتی ­اکسیدانی کاتالاز، سوپراکسید دیسموتاز، پراکسیداز و پلی­ فنل­ اکسیداز را تغییر داد. همچنین تیمار کادمیوم محتوای ترکیبات آنتی ­اکسیدانی غیرآنزیمی مانند فنل­ ها، فلاونوئیدها، آنتوسیانین­ ها، پروتئین­ های محلول و پرولین را افزایش داد. علاوه بر این، کاهش درصد جوانه­ زنی بذر و پارامترهای رشدی و نیز کاهش محتوای رنگیزه ­های فتوسنتزی کلروفیل و کاروتنوئید از اثرات سمی کادمیوم بود.

Keywords [فارسی]

  • آنتی اکسیدان های غیر آنزیمی
  • آنزیم های آنتی اکسیدانی
  • پرولین
  • ترکیبات فنلی
  • شنبلیله
Azarmehr BA, Taghizadeh M, Karimi F, and Gargari SLM, 2013. Secondary metabolite contents and antioxidant enzyme activities of Cichorium intybus hairy roots in response to zinc. Journal of Medicinal Plants and By-Products 2: 131-138.
Bates LS, Waldren RP, and Teare ID, 1973. Rapid determination of free proline for water-stress studies. Plant and Soil 39: 205-207.
Begum N, Afzal S, Zhao H, Lou L, and Cai Q, 2018. Shoot endophytic plant growth-promoting bacteria reduce cadmium toxicity and enhance switchgrass (Panicum virgatum L.) biomass. Acta Physiologiae Plantarum 40: 1-16.
Benabid H and Fouzi Ghorab M, 2012. Is the decrease of chlorophyll first alert of pollution? A study of the effect of cadmium on chlorophyll content of Canadian wonder beans (Phaseolus vulgaris). 4th International Conference on Chemical, Biological, and Environmental Engineering, Sep. 1- Sep. 2, Phuket, Thailand, pp. 279-282.
Bradbeer JW, 1988. Seed Dormancy and Germination. Blackie and Son Ltd., Glasgow, UK. 146 p.
Bradford MM, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-254.
Branch S, 2013. Fenugreek (Trigonella foenum-graecum L.) as a valuable medicinal plant. International Journal of Advanced Biological and Biomedical Research 1: 922-931.
Chaca MP, Vigliocco A, Reinoso H, Molina A, Abdala G, Zirulnik F, and Pedranzani H, 2014. Effects of cadmium stress on growth, anatomy, and hormone contents in Glycine max (L.) Merr. Acta Physiologiae Plantarum 36: 2815-2826.
Chang CC, Yang MH, Wen HM, and Chern JC, 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis 10: 178-182.
Dawuda MM, Liao W, Hu L, Yu J, Xie J, Calderón-Urrea A, Wu Y, and Tang Z, 2020. Foliar application of abscisic acid mitigates cadmium stress and increases food safety of cadmium-sensitive lettuce (Lactuca sativa L.) genotype. Peer Journal 8: e9270.
Giannopolitis CN and Ries SK, 1977. Superoxide dismutases: I. Occurrence in higher plants. Plant Physiology 59: 309-314.
Guo H, Hong C, Chen X, Xu Y, Liu Y, Jiang D, and Zheng B, 2016. Different growth and physiological responses to cadmium of the three Miscanthus species. PloS One 11: e0153475.
Hatamian M, Rezaei Nejad A, Kafi M, Souri MK, and Shahbazi K, 2020. Interaction of lead and cadmium on growth and leaf morphophysiological characteristics of European hackberry (Celtis australis) seedlings. Chemical and Biological Technologies in Agriculture 7: 1-8.
Heath RL and Packer L, 1968. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125: 189-198.
Hussain I, Akhtar S, Ashraf MA, Rasheed R, Siddiqi EH, and Ibrahim M, 2013. Response of maize seedlings to cadmium application after different time intervals. International Scholarly Research Notices, ID 169610: 9 pages.
Jan S, Alyemeni MN, Wijaya L, Alam P, Siddique KH, and Ahmad P, 2018. Interactive effect of 24-epibrassinolide and silicon alleviates cadmium stress via the modulation of antioxidant defense and glyoxalase systems and macronutrient content in Pisum sativum L. seedlings. BMC Plant Biology 18: 1-18.
Jan R, Khan MA, Asaf S, Lee IJ, and Kim KM, 2019. Metal resistant endophytic bacteria reduces cadmium, nickel toxicity, and enhances expression of metal stress related genes with improved growth of Oryza sativa, via regulating its antioxidant machinery and endogenous hormones. Plants 8: 363-380.
MacAdam JW, Nelson CJ, and Sharp RE, 1992. Peroxidase activity in the leaf elongation zone of tall fescue: I. Spatial distribution of ionically bound peroxidase activity in genotypes differing in length of the elongation zone. Plant Physiology 99: 872-878.
Mehrafarin A, Rezazadeh SH, Naghdi Badi H, Noormohammadi GH, Zand E, and Qaderi A, 2011. A review on biology, cultivation and biotechnology of fenugreek (Trigonella foenum-graecum L.) as a valuable medicinal plant and multipurpose. Journal of Medicinal Plants 10: 6-24.
Mukherjee M, 2017. Lead and cadmium toxicity on seedling growth and metabolism of Trigonella foenum-graecum L. International Journal of Science and Research 6: 1685-1689.
Ozyigit İİ, Abakirova A, Hocaoglu-Ozyigit A, Kurmanbekova G, Chekirov K, Yalcin B, and Yalcin İE, 2021. Cadmium stress in barley seedlings: accumulation, growth, anatomy, and physiology. International Journal of Life Sciences and Biotechnology 4: 186-205.
Plessi M, Bertelli D, and Albasini A, 2007. Distribution of metals and phenolic compounds as a criterion to evaluate variety of berries and related jams. Food Chemistry 100: 419-427.
Prasad MNV, 1995: Cadmium toxicity and tolerance in vascular plants. Environmental and Experimental Botany 35: 525-545.
Raymond J, Rakariyatham N, and Azanza JL, 1993. Purification and some properties of polyphenol oxidase from sunflower seeds. Phytochemistry 34: 927-931.
Riskuwa-Shehu ML, Ismail HY, and Ijah UJJ, 2020: Heavy metal resistance by endophytic bacteria isolated from guava (Psidium guajava) and mango (Mangifera indica) leaves. International Annals of Science 9: 16-23.
Salarizadeh S, Kavousi HR, and Pourseyadi S, 2016. Effect of cadmium on germination characters and biochemical parameters of two Iranian ecotypes of cumin (Cuminum cyminum L.). Journal of Medicinal Plants and By-products 5: 15-22.
Shah AA, Khan WU, Yasin NA, Akram W, Ahmad A, Abbas M, Ali A, and Safdar MN, 2020. Butanolide alleviated cadmium stress by improving plant growth, photosynthetic parameters, and antioxidant defense system of Brassica oleracea. Chemosphere 261: 127728.
Shah K, Mankad AU, and Reddy MN, 2017. Cadmium accumulation and its effects on growth and biochemical parameters in Tagetes erecta L. Journal of Pharmacognosy and Phytochemistry 6: 111-115.
Sumanta N, Haque CI, Nishika J, and Suprakash R, 2014. Spectrophotometric analysis of chlorophylls and carotenoids from commonly grown fern species by using various extracting solvents. Research Journal of Chemical Sciences 2231: 606X.
Ullah I, Al-Johny BO, Al-Ghamdi KM, Al-Zahrani HAA, Anwar Y, Firoz A, Al-Kenani N, and Almatry MAA, 2019. Endophytic bacteria isolated from Solanum nigrum L., alleviate cadmium (Cd) stress response by their antioxidant potentials, including SOD synthesis by sodA gene. Ecotoxicology and Environmental Safety 174: 197-207.
Velikova V, Yordanov I, and Edreva A, 2000. Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective role of exogenous polyamines. Plant Science 151: 59-66.
Wagner GJ, 1979. Content and vacuole/extravacuole distribution of neutral sugars, free amino acids, and anthocyanin in protoplasts. Plant Physiology 64: 88-93.
Wang Q, Ge C, Wu Y, Sahito ZA, Ma L, Pan F, Zhou Q, Huang L, Feng Y, and Yang X, 2020. The endophytic bacterium Sphingomonas SaMR12 alleviates Cd stress in oilseed rape through regulation of the GSH-AsA cycle and antioxidative enzymes. BMC Plant Biology 20: 1-14.
Yildirim E, Ekinci M, Turan M, Güleray AGAR, Selda ÖRS, Dursun A, Raziye KUL, and Balci T, 2019. Impact of cadmium and lead heavy metal stress on plant growth and physiology of rocket (Eruca sativa L.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 22: 843-850.
Zandi P, Basu SK, Cetzal-Ix W, Kordrostami M, Chalaras SK, and Khatibai LB, 2017. Fenugreek (Trigonella foenum‐graecum L.): an important medicinal and aromatic crop. Active Ingredients from Aromatic and Medicinal Plants 207-224.
Zayneb C, Bassem K, Zeineb K, Grubb CD, Noureddine D, Hafedh M, and Amine E, 2015. Physiological responses of fenugreek seedlings and plants treated with cadmium. Environmental Science and Pollution Research 22: 10679-10689.