Silicon nanoparticles alleviate arsenic toxicity in rice (Oryza sativa L.)

Document Type : Research Paper


1 Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran

2 Department of Biology, Science and Research Branch, Islamic Azad University, Teheran, Iran


Arsenic (As) is one of the most hazardous metalloids for plants, however, little is understood about the role of silicon nanoparticles (Si-NPs) in improving rice tolerance under As toxicity. An experiment was conducted in 2020 at Islamshahr Branch, Islamic Azad University, Islamshahr, Iran, to examine the impacts of As (50 M) and Si-NPs (50 and 100 mg/L) on rice growth, chlorophyll and proline metabolism, antioxidant defense system, ionic homeostasis, and expression of Si/As transporters under hydroponic conditions. The results showed that Si-NPs by boosting the activities of antioxidant enzymes, diminished hydrogen peroxide and superoxide anion, and hence, protected the photosynthetic apparatus and enhanced plant growth during As toxicity. Si-NPs increased Si uptake and declined As uptake in As-treated seedlings by adjusting the relative expression of Si/As transporters (Lsi1, Lsi2, Lsi6). Si-NPs maintained ionic homeostasis under As stress by increasing the uptake of mineral nutrients. In general, Si-NPs increased rice growth and biomass during As toxicity, which might be exploited to develop effective fertilizers to improve crop growth and yield in As-contaminated areas.


Article Title [Persian]

تاثیر نانوذرات سیلیس بر کاهش سمیت آرسنیک در برنج (Oryza sativa L.)

Authors [Persian]

  • طاهره کیانی 1
  • لیلا پیشکار 1
  • نسرین سرتیپ نیا 1
  • علیرضا ایران بخش 2
  • گیتی برزین 1
1 گروه زیست شناسی، واحد اسلامشهر، دانشگاه آزاد اسلامی، اسلامشهر
2 گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران
Abstract [Persian]

 آرسنیک یکی از سمی ­ترین متالوئیدها برای گیاهان شناخته شده است، ولی اطلاعات کمی در مورد استفاده از نانوذرات سیلیس در کاهش سمیت آرسنیک در برنج  وجود دارد. این تحقیق به منظور بررسی اثرات غلظت­ های نانوذرات سیلیس (50 و 10 میلی­گرم بر لیتر) و آرسنیک (50 میکرومولار) بر رشد گیاه، متابولیسم کلروفیل و پرولین، سیستم دفاعی آنتی اکسیدانی، همئوستازی یونی و بیان ناقل­ های سیلیس/آرسنیک در برنج در شرایط هیدروپونیک در سال 1400 در دانشگاه آزاد اسلامی واحد اسلامشهر انجام شد. نتایج نشان داد که افزودن نانوذرات سیلیس به محیط کشت هیدروپونیک با تنظیم فعالیت آنزیم­ های آنتی اکسیدانی، سطح پراکسید هیدروژن و سوپراکسید آنیون را کاهش داد و از دستگاه فتوسنتزی محافظت کرده و باعث بهبود رشد گیاه تحت تنش آرسنیک شد. نانوذرات سیلیس با تعدیل بیان ناقلین سیلیس/آرسنیک (Lsi1 ،Lsi2، Lsi6)، باعث کاهش جذب آرسنیک و افزایش جذب سیلیس در گیاهان برنج تحت تنش آرسنیک شد. کاربرد نانوذرات سیلیس با افزایش جذب عناصر مغذی، باعث حفظ همئوستازی یونی در گیاه برنج تحت تنش آرسنیک شد. به طور کلی، نانوذرات سیلیس باعث افزایش رشد برنج تحت تنش آرسنیک شد، که می­تواند برای طراحی کودهای موثر برای افزایش رشد و عملکرد دانه در مناطق آلوده به آرسنیک استفاده شود.

Keywords [Persian]

  • آرسنیک
  • برنج
  • تنش اکسیداتیو
  • ناقلین سیلیس/آرسنیک
  • نانوذرات
Ali S, Rizwan M, Hussain A, Zia Ur Rehman M, Ali B, Yousaf B, Wijaya L, Alyemeni MN, and Ahmad P, 2019.  Silicon nanoparticles enhanced the growth and reduced the cadmium accumulation in grains of         wheat (Triticum aestivum L.). Plant Physiology and Biochemistry 140: 1–8.
Bates LS, Waldren RP, and Teare ID, 1973. Rapid determination of free proline for water-stress studies. Plant and Soil 39: 205–207.
Bidi H, Fallah H, Niknejad Y, and Barari Tari D, 2021. Iron oxide nanoparticles alleviate arsenic phytotoxicity in rice by improving iron uptake, oxidative stress tolerance and diminishing arsenic accumulation. Plant Physiology and Biochemistry 163: 348–357.
Chance B and Maehly AC, 1955. Assay of catalases and peroxidase. Methods in Enzymology 2: 764– 775.
Chen R, Zhang C, Zhao Y, Huang Y, and Liu Z, 2018. Foliar application with nano-silicon reduced cadmium accumulation in grains by inhibiting cadmium translocation in rice plants. Environmental Science and Pollution Research 25: 2361–2368.
Cui J, Liu T, Li F, Yi J, Liu C, and Yu H, 2017. Silica nanoparticles alleviate cadmium toxicity in rice cells: Mechanisms and size effects. Environmental Pollution 228: 363–369.
De la Rosa G, García-Castañeda C, Vázquez-Núñez E, Alonso-Castro ÁJ, Basurto-Islas G, Mendoza Á, Cruz-Jiménez G, and Molina C, 2017. Physiological and biochemical response of plants to engineered NMs: Implications on future design. Plant Physiology and Biochemistry 110: 226–235.
Elstner EF and Heupel A, 1976. Inhibition of nitrite formation from hydroxylammoniumchloride: a simple assay for superoxide dismutase. Analytical Biochemistry 70: 616–620.
Ghasemi-Omran VO, Ghorbani A, and Sajjadi-Otaghsara SA, 2021. Melatonin alleviates NaCl-induced damage by regulating ionic homeostasis, antioxidant system, redox homeostasis, and expression of steviol glycosides-related biosynthetic genes in in vitro cultured Stevia rebaudiana Bertoni. In Vitro Cellular & Developmental Biology - Plant 57: 319–331.
Ghorbani A, Ghasemi Omran VO, Razavi SM, Pirdashti H, and Ranjbar M, 2019. Piriformospora indica confers salinity tolerance on tomato (Lycopersicon esculentum Mill.) through amelioration of nutrient accumulation, K+/Na+ homeostasis and water status. Plant Cell Reports 38: 1151–1163.
Ghorbani A, Pishkar L, Roodbari N, Ali Tavakoli S, Moein Jahromi E, and Chu W, 2022. Nitrate reductase is needed for methyl jasmonate-mediated arsenic toxicity tolerance of rice by modulating the antioxidant defense system, glyoxalase system and arsenic sequestration mechanism. Journal of Plant Growth Regulation.
Ghorbani A, Pishkar L, Roodbari N, Pehlivan N, and Wu C, 2021. Nitric oxide could allay arsenic phytotoxicity in tomato (Solanum lycopersicum L.) by modulating photosynthetic pigments, phytochelatin metabolism, molecular redox status and arsenic sequestration. Plant Physiology and Biochemistry 167: 337–348.
Ghorbani A, Razavi SM, Ghasemi Omran VO, and Pirdashti H, 2018. Piriformospora indica inoculation alleviates the adverse effect of NaCl stress on growth, gas exchange and chlorophyll fluorescence in tomato (Solanum lycopersicum L.). Plant Biology 20: 729–736.
Ghorbani A, Tafteh M, Roudbari N, Pishkar L, Zhang W, and Wu C, 2020. Piriformospora indica augments arsenic tolerance in rice (Oryza sativa) by immobilizing arsenic in roots and improving iron translocation to shoots. Ecotoxicology and Environmental Safety 209: 111793.
Ghorbani A, Zarinkamar F, and Fallah A, 2009. The effect of cold stress on the morphologic and physiologic characters of two rice varieties in seedling stage. Journal of Crop Breeding (In Persian with English abstract) 1: 50–66.
Ghorbani A, Zarinkamar F, and Fallah A, 2011. Effect of cold stress on the anatomy and morphology of the tolerant and sensitive cultivars of rice during germination. Journal of Cell and Tissue 2(3): 235–244 (In Persian with English abstract).
Hoagland D and Arnon D, 1941. Physiological aspects of availability of nutrients for plant growth. Soil Science 51: 431–444.
Juárez-Maldonado A, Ortega-Ortiz H, González-Morales S, Morelos-Moreno Á, Cabrera-de la Fuente M, Sandoval-Rangel A, Cadenas-Pliego G, and Benavides-Mendoza A, 2019. Nanoparticles and nanomaterials as plant biostimulants. International Journal of Molecular Sciences 20: 162.
Khan E and Gupta M, 2018. Arsenic–silicon priming of rice (Oryza sativa L.) seeds influence mineral nutrient uptake and biochemical responses through modulation of Lsi-1, Lsi-2, Lsi-6 and nutrient transporter genes. Scientific Reports 8: 10301.
Khan ZS, Rizwan M, Hafeez M, Ali S, Adrees M, Qayyum MF, Khalid S, Ur Rehman MZ, and Sarwar MA, 2020. Effects of silicon nanoparticles on growth and physiology of wheat in cadmium contaminated soil under different soil moisture levels. Environmental Science and Pollution Research 27(5): 4958–4968.
Koleva L, Umar A, Yasin NA, Shah AA, Siddiqui MH, Alamri S, Riaz L, Raza A, Javed T, and Shabbir Z, 2022. Iron oxide and silicon nanoparticles modulate mineral nutrient homeostasis and metabolism in cadmium-stressed Phaseolus vulgaris. Frontiers in Plant Science 13: 806781.
Ma JF, Yamaji N, Tamai K, and Mitani N, 2007. Genotypic difference in silicon uptake and expression of silicon transporter genes in rice. Plant Physiology 145(3): 919–924.
Miyake C and Asada K, 1992. Thylakoid-bound ascorbate peroxidase in spinach chloroplasts and photoreduction of its primary oxidation product monodehydroascorbate radicals in thylakoids. Plant and Cell Physiology 33: 541–553.
Mousavi SR, Niknejad Y, Fallah H, and Barari-Tari D, 2020. Methyl jasmonate alleviates arsenic toxicity in rice. Plant Cell Reports 39: 1041–1060.
Nakano Y and Asada K, 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant & Cell Physiology 22: 867–880.
Otero XL, Tierra W, Atiaga O, Guanoluisa D, Nunes LM, Ferreira TO, and Ruales J, 2016. Arsenic in rice agrosystems (water, soil and rice plants) in Guayas and Los Ríos provinces, Ecuador. Science of the Total Environment 573: 778–787.
Pandey AK, Gedda MR, and Verma AK, 2020. Effect of arsenic stress on expression pattern of a rice specific miR156j at various developmental stages and their allied co-expression target networks. Frontiers in Plant Science 11: 752.
Ramezani M, Enayati M, Ramezani M, and Ghorbani A, 2021. A study of different strategical views into heavy metal (oid) removal in the environment. Arabian Journal of Geosciences 14: 2225.
Rizwan M, Ali S, Ur Rehman MZ, Malik S, Adrees M, Qayyum MF, Alamri SA, Alyemeni MN, and Ahmad P, 2019. Effect of foliar applications of silicon and titanium dioxide nanoparticles on growth, oxidative stress, and cadmium accumulation by rice (Oryza sativa). Acta Physiologiae Plantarum 41: 35.
Ruíz-Huerta EA, de la Garza Varela A, Gómez-Bernal JM, Castillo F, Avalos-Borja M, SenGupta B, and Martínez-Villegas N, 2017. Arsenic contamination in irrigation water, agricultural soil and maize crop from an abandoned smelter site in Matehuala, Mexico. Journal of Hazardous Materials 339: 330–339.
Sharma DK, Andersen SB, Ottosen CO, and Rosenqvist E, 2012. Phenotyping of wheat cultivars for heat tolerance using chlorophyll a fluorescence. Functional Plant Biology 39(11): 936–947.
Sinha S, Saxena R, and Singh S, 2005. Chromium induced lipid peroxidation in the plants of Pistia stratiotes L.: role of antioxidants and antioxidant enzymes. Chemosphere 58: 595–604.
Tripathi DK, Singh S, Singh VP, Prasad SM, Chauhan DK, and Dubey NK, 2016. Silicon nanoparticles more efficiently alleviate arsenate toxicity than silicon in maize cultivar and hybrid differing in arsenate tolerance. Frontiers in Environmental Science 4: 46.
Tripathi P, Tripathi RD, Singh RP, Dwivedi S, Goutam D, Shri M, Trivedi PK, and Chakrabarty D, 2013. Silicon mediates arsenic tolerance in rice (Oryza sativa L.) through lowering of arsenic uptake and improved antioxidant defence system. Ecological Engineering 52: 96–103.
Yamaji N, Mitatni N, and Ma JF, 2008. A transporter regulating silicon distribution in rice shoots. The Plant Cell 20(5): 1381–1389.
Zemanová V, Popov M, Pavlíková D, Kotrba P, Hnilička F, Česká J, and Pavlík M, 2020. Effect of arsenic stress on 5-methylcytosine, photosynthetic parameters and nutrient content in arsenic hyperaccumulator Pteris cretica (L.) var. Albo-lineata. BMC Plant Biology 20(1): 130.
Zhang W, Long J, Geng J, Li J, and Wei Z, 2020. Impact of titanium dioxide nanoparticles on Cd phytotoxicity and bioaccumulation in rice (Oryza sativa L.). The International Journal of Environmental Research and Public Health 17(9): 2979.