Transcriptional changes of AOS, AOC, OMT, NHX1, and L1S1 genes in roots of barley genotypes under salinity stress

Document Type : Research Paper


1 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz 51666, Iran; Department of Biology, Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran

2 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz 51666, Iran; Center of Excellence in Cereal Molecular Breeding, University of Tabriz, Tabriz 51666, Iran


Salinity is a big problem for agriculture and crop productivity worldwide. Barley is considered notably salt-tolerant and there is considerable genetic variation in barley in response to salinity. In the present study, the expression pattern of AOS, AOC, OMT, L1S1, and NHX1 genes were investigated in the roots of three barley genotypes, Sahara3771, Clipper, and an advanced breeding line (A-line) 24 hours, 3 days, and 3 weeks after 100 and 200 mM NaCl treatments as well as control (no NaCl). Analysis of variance revealed significant salinity x genotype x salt exposure time interaction for all the studied genes, except AOC. The highest expression level of the AOC gene was noted under 200 mM NaCl in Clipper and the lowest expression was recorded under 200 mM and 100 mM NaCl in A-line and Clipper, respectively. For the AOS gene, the highest expression level was recorded 3 weeks after 200 mM NaCl treatment. The maximum expression level of NHX1 was measured in A-Line 24 hours after 100 mM NaCl treatment and the lowest in Sahara3771 3 weeks after 200 mM NaCl treatment. The OMT gene showed the highest expression in Sahara3771 3 weeks after 100 mM NaCl treatment and the lowest was observed in A-Line under 200 and 100 mM NaCl. For the LlS1 gene, the highest level of the transcripts was measured 3 weeks after 100 mM NaCl treatment. LlS1 gene showed the lowest expression level 24 hours after 200 mM NaCl in Sahara3771.


Article Title [فارسی]

تغییرات رونویسی ژن های AOS،AOC ، OMT، NHX1 و L1S1 در ریشه ژنوتیپ های جو تحت تنش شوری

Authors [فارسی]

  • سارا غفاریان 1
  • سید ابوالقاسم محمدی 2
  • محمود تورچی 2
1 گروه به نژادی و بیوتکنولوژی گیاهی، دانشکده کشاورزی، دانشگاه تبریز، تبریز؛ گروه زیست شناسی، دانشکده علوم پایه، دانشگاه شهید مدنی آذربایجان، تبریز
2 گروه به‌نژادی و بیوتکنولوژی گیاهی، دانشکده کشاورزی، دانشگاه تبریز، تبریز؛ قطب علمی اصلاح مولکولی غلات، دانشگاه تبریز، تبریز
Abstract [فارسی]

شوری یک مشکل عمده برای کشاورزی و تولید محصول در سطح جهان است. جو به عنوان گیاهی با تحمل بالا به شوری، تنوع ژنتیکی قابل توجهی در پاسخ به شوری دارد. در مطالعه حاضر، الگوی بیان ژن­ های AOS ،AOC  OMT  ،NHX1 و L1S1 در ریشه سه ژنوتیپ­ جو، Sahara3771  ،Clipper و لاین اصلاحی پیشرفته، 24 ساعت، سه روز و سه هفته پس از اعمال شوری 100 و 200 میلی ­مولار NaCl در مقایسه با کنترل مورد بررسی قرار گرفت. تجزیه واریانس نشان دهنده اثر متقابل سه جانبه شوری × ژنوتیپ × مدت زمان معنی­ دار برای کلیه ژن­ های مورد مطالعه غیر از AOC بود. بیشترین بیان ژن AOC تحت شوری 200 میلی مولار NaCl در NaCl در ژنوتیپ­ Clipper و کمترین میزان بیان آن تحت تیمار 200 و 100 میلی مولار NaCl به ترتیب در لاین اصلاحی پیشرفته و Clipper مشاهده شد. بیشترین میزان بیان ژن AOS سه هفته پس از اعمال تیمار 200 میلی­ مولار NaCl به دست آمد. حداکثر و حداقل بیان ژن  NHX1 به ترتیب در لاین اصلاحی پیشرفته 24 ساعت پس از تیمار 100 میلی مولار NaCl و در Sahara3771 سه هفته پس از اعمال شوری 200 میلی مولار مشاهده شد. حداکثر بیان ژن OMT در Sahara3771 سه هفته پس از اعمال شوری 100 میلی مولار NaCl و کمترین میزان  بیان آن در لاین اصلاحی پیشرفته تحت تیمارهای 100 و 200 میلی مولار NaCl به دست آمد. برای ژن L1S1 بیشترین میزان بیان سه هفته پس از اعمال شوری 100 میلی­ مولار NaCl و کمترین بیان آن 24 ساعت پس از اعمال شوری 200 میلی مولار در Sahara3771 حاصل شد.

Keywords [فارسی]

  • بیان ژن
  • تنش شوری
  • Hordeum vulgae
  • Real-time RT-PCR
Aharon R, Shahak Y, Wininger S, Bendov R, Kapulnik Y, and Galili G, 2003. Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress. The Plant Cell 15: 439-447.
Assaha DVM, Ueda A, Saneoka H, Al-Yahyai R, and Yaish MW, 2017. The role of Na+ and K+ transporters in salt stress adaptation in glycophytes. Frontiers in Physiology 8: 509.
Barkla BJ and Blumwald E, 1991. Identification of a 170-kDa protein associated with the vacuolar Na+/H+ antiport of Beta vulgaris.  Proceedings of the National Academy of Sciences of the United States of America 88: 11177-11181.
 Brash AR, Baertschi SW, Ingram CD, and Harris TM, 1988. Isolation and characterization of natural allene oxides: unstable intermediates in the metabolism of lipid hydroperoxides. Proceedings of the National Academy of Sciences of the United States of America 85: 3382-3386.
Fukuda A, Chiba K, Maeda M, Nakamura A, Maeshima M, and Tanaka Y, 2004. Effect of salt and osmotic stresses on the expression of genes for the vacuolar H+-pyrophosphatase, H+-ATPase subunit A and Na+/H+ antiporter from barley. Journal of Experimental Botany 55: 585-94.
Greenway H, 1965. Plant response to saline substrates. Growth and ion uptake throughout plant development in two varieties of Hordeum vulgare. Australian Journal of Biological Sciences 18: 763-779.
Hamberg M and Fahlstadius P, 1990. Allene oxide cyclase: a new enzyme in plant lipid metabolism. Archives of Biochemistry and Biophysics 276: 518-526.
Ibrahim RK and Muzac I, 2000. The methyltransferase gene superfamily: a tree with multiple branches. In: Romeo JT, Ibrahim R, Luc V, and Luca VD (Eds).  Evolution of metabolic pathways. Pp. 349–384. Pergamon Press, New York, USA.
Isayenkov SV, 2012. Physiological and molecular aspects of salt stress in plants. Cytology and Genetics 46: 302-318.
 Lam KC, Ibrahim RK, Behdad B, and Dayanandan S, 2007. Structure, function, and evolution of plant O-methyltransferases. Genome 50: 1001-1013.
Ligaba A and Katsuhara M, 2010. Insights into the salt tolerance mechanism in barley (Hordeum vulgare L.) from comparisons of cultivars that differ in salt sensitivity. Journal of Plant Research 123: 105-118.
Ligabaa A, Katsuharab M, Shibasakab M, and Djirac G, 2011. Abiotic stresses modulate expression of major intrinsic proteins in barley (Hordeum vulgare L.). Comptes Rendus Biologies 334: 127-139.
Livak KJ and Schmittgen TD, 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods 25: 402-408.
Munns R, Gardner PA, Tonnet ML, and Rawson HM, 1988. Growth and development in NaCl-treated plants. II. Do Na+ or Cl− concentrations in dividing or expanding tissues determine growth in barley? Australian Journal of Plant Physiology 15: 529-540.
Pardo JM, Cubero B, and Leidi EO, 2006. Alcalication exchangers: roles in cellular homeostasis and stress tolerance. Journal of Experimental Botany 57: 1181-1199.
Pedranzani H, Racagni G, Alemano S, Miersch O, Ramírez I Peña-Cortés H, Taleisnik E Machado-Domenech E, and Abdala G, 2003. Salt tolerant tomato plants show increased levels of jasmonic acid. Plant Growth Regulation 41: 149-158.
Schaller A and Stintzi A, 2009. Enzymes in jasmonate biosynthesis: structure, function, regulation. Phytochemistry 70: 1532-1538.
Shahid M and Jaradat AA, 2013. Barley: a salt tolerant cereal crop. Biosalinity News 14: 1-2.
Sugimoto M, Okada Y, Sato K, Ito K, and Takeda K, 2003. A root-specific O-methyltransferase gene expressed in salt-tolerant barley. Bioscience, Biotechnology and Biochemistry 67: 966-72.
 Tang C, Wang X, Duan X, Wang X, Huang L, and Kang Z, 2013. Functions of the lethal leaf-spot1 gene in wheat cell death and disease tolerance to Puccinia striiformis. Journal of Experimental Botany 64: 2955-2969.
Temel A and Gozukirmizi N, 2005. Physiological and molecular changes in barley and wheat under salinity. Applied Biochemistry and Biotechnology 175: 2950-2960.
Teaster M and Davenport R, 2003. Na+ tolerance and Na+ transport in higher plants. Annals of Botany 91: 503-527.
Ueda A, Kathiresan A, Inada M, Narita Y, Nakamura T, Shi W, Takabe T, and Bennett J, 2004. Osmotic stress in barley regulates expression of a different set of genes than salt stress does. Journal of Experimental Botany 55: 2213-2218.
Walia H, Wilson C, Wahid A, Condamine P, Cui X, and Close TJ, 2006. Expression analysis of barley (Hordeum vulgare L.) during salinity stress. Functional and Integrative Genomics 6: 143-156.
 Xu R, Wang J, Li C, Johnson P, Lu C, and Zhou M, 2012. A single locus is responsible for salinity tolerance in a Chinese landrace barley (Hordeum vulgare L.). PLoS ONE e43079.
Zhao Y, Dong W, Zhang N, Ai X, Wang M, Huang Z, Xiao L, and Xia G, 2014. A wheat allene oxide cyclase gene enhances salinity tolerance via jasmonate signaling. Plant Physiology 164: 1068-1076.