Transcriptional changes of AOS, AOC, OMT, NHX1, and L1S1 genes in roots of barley genotypes under salinity stress

Document Type : Research Paper

Authors

1 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz 51666, Iran; Department of Biology, Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran

2 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz 51666, Iran; Center of Excellence in Cereal Molecular Breeding, University of Tabriz, Tabriz 51666, Iran

Abstract

Salinity is a big problem for agriculture and crop productivity worldwide. Barley is considered notably salt-tolerant and there is considerable genetic variation in barley in response to salinity. In the present study, the expression pattern of AOS, AOC, OMT, L1S1, and NHX1 genes were investigated in the roots of three barley genotypes, Sahara3771, Clipper, and an advanced breeding line (A-line) 24 hours, 3 days, and 3 weeks after 100 and 200 mM NaCl treatments as well as control (no NaCl). Analysis of variance revealed significant salinity x genotype x salt exposure time interaction for all the studied genes, except AOC. The highest expression level of the AOC gene was noted under 200 mM NaCl in Clipper and the lowest expression was recorded under 200 mM and 100 mM NaCl in A-line and Clipper, respectively. For the AOS gene, the highest expression level was recorded 3 weeks after 200 mM NaCl treatment. The maximum expression level of NHX1 was measured in A-Line 24 hours after 100 mM NaCl treatment and the lowest in Sahara3771 3 weeks after 200 mM NaCl treatment. The OMT gene showed the highest expression in Sahara3771 3 weeks after 100 mM NaCl treatment and the lowest was observed in A-Line under 200 and 100 mM NaCl. For the LlS1 gene, the highest level of the transcripts was measured 3 weeks after 100 mM NaCl treatment. LlS1 gene showed the lowest expression level 24 hours after 200 mM NaCl in Sahara3771.
 

Keywords


Article Title [فارسی]

تغییرات رونویسی ژن های AOS،AOC ، OMT، NHX1 و L1S1 در ریشه ژنوتیپ های جو تحت تنش شوری

Authors [فارسی]

  • سارا غفاریان 1
  • سید ابوالقاسم محمدی 2
  • محمود تورچی 2
1 گروه به نژادی و بیوتکنولوژی گیاهی، دانشکده کشاورزی، دانشگاه تبریز، تبریز؛ گروه زیست شناسی، دانشکده علوم پایه، دانشگاه شهید مدنی آذربایجان، تبریز
2 گروه به‌نژادی و بیوتکنولوژی گیاهی، دانشکده کشاورزی، دانشگاه تبریز، تبریز؛ قطب علمی اصلاح مولکولی غلات، دانشگاه تبریز، تبریز
Abstract [فارسی]

شوری یک مشکل عمده برای کشاورزی و تولید محصول در سطح جهان است. جو به عنوان گیاهی با تحمل بالا به شوری، تنوع ژنتیکی قابل توجهی در پاسخ به شوری دارد. در مطالعه حاضر، الگوی بیان ژن­ های AOS ،AOC  OMT  ،NHX1 و L1S1 در ریشه سه ژنوتیپ­ جو، Sahara3771  ،Clipper و لاین اصلاحی پیشرفته، 24 ساعت، سه روز و سه هفته پس از اعمال شوری 100 و 200 میلی ­مولار NaCl در مقایسه با کنترل مورد بررسی قرار گرفت. تجزیه واریانس نشان دهنده اثر متقابل سه جانبه شوری × ژنوتیپ × مدت زمان معنی­ دار برای کلیه ژن­ های مورد مطالعه غیر از AOC بود. بیشترین بیان ژن AOC تحت شوری 200 میلی مولار NaCl در NaCl در ژنوتیپ­ Clipper و کمترین میزان بیان آن تحت تیمار 200 و 100 میلی مولار NaCl به ترتیب در لاین اصلاحی پیشرفته و Clipper مشاهده شد. بیشترین میزان بیان ژن AOS سه هفته پس از اعمال تیمار 200 میلی­ مولار NaCl به دست آمد. حداکثر و حداقل بیان ژن  NHX1 به ترتیب در لاین اصلاحی پیشرفته 24 ساعت پس از تیمار 100 میلی مولار NaCl و در Sahara3771 سه هفته پس از اعمال شوری 200 میلی مولار مشاهده شد. حداکثر بیان ژن OMT در Sahara3771 سه هفته پس از اعمال شوری 100 میلی مولار NaCl و کمترین میزان  بیان آن در لاین اصلاحی پیشرفته تحت تیمارهای 100 و 200 میلی مولار NaCl به دست آمد. برای ژن L1S1 بیشترین میزان بیان سه هفته پس از اعمال شوری 100 میلی­ مولار NaCl و کمترین بیان آن 24 ساعت پس از اعمال شوری 200 میلی مولار در Sahara3771 حاصل شد.
 

Keywords [فارسی]

  • بیان ژن
  • تنش شوری
  • Hordeum vulgae
  • Real-time RT-PCR
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