Effect of chitosan on regeneration and secondary metabolite production of Lilium regale

Document Type : Research Paper


University of Mohaghegh Ardabili, Ardabil, Iran


Lilium spp. belong to the Liliaceae family. This plant contains a valuable secondary metabolite which can be increased by the stimuli treatment. The main goal of the present study was to evaluate the effects of chitosan as a stimulant on regeneration and production of secondary metabolites of Lilium regale. The experiment was carried out as a completely randomized design in the MS medium with 10 replications. Five different concentrations (0, 50, 100, 150, and 200 mg/L) of chitosan were used as treatments. Different morphological traits, leaf chlorophyll, and secondary metabolites were measured. Analysis of variance showed that chitosan significantly affected plant height, fresh weight, bulblet number, root number, chlorophyll a and b, total chlorophyll, phenols, flavonoids, and regeneration percentage. The highest and lowest fresh weight and root number were obtained from 200 ppm of chitosan and the control, respectively. The results also showed that the plant height and bulblet number in all concentrations of chitosan were higher than the control. The highest and lowest regeneration rate were obtained from the 200 ppm chitosan and the control, respectively. However, the highest amount of phenols, flavonoids, and chlorophyll was obtained from the 200 ppm chitosan. According to the results of the present study, chitosan at the rate of 200 ppm had a more positive effect on regeneration and production of secondary metabolites of Lilium regale compared to other concentrations.


Article Title [Persian]

بررسی تأثیر کیتوسان بر باززایی و تولید متابولیت های ثانویه در گل سوسن گونه رگال

Authors [Persian]

  • یونس پوربیرامی هیر
  • مهسا خلفی
  • اسماعیل چمنی
  • حسن ملکی لجایر
دانشگاه محقق اردبیلی، اردبیل
Abstract [Persian]

گونه­ های  Lilium متعلق به خانواده Liliaceae هستند. این گیاه حاوی یک متابولیت ثانویه ارزشمند است که با تیمار محرک می­ توان آن را افزایش داد. هدف اصلی مطالعه حاضر بررسی اثرات کیتوزان به عنوان یک محرک بر باززایی و تولید متابولیت های ثانویه در  Lilium regale  بود. آزمایش در قالب طرح کاملا تصادفی در محیط کشت MS با 10 تکرار انجام شد. پنج غلظت مختلف (0، 50، 100، 150 و 200 میلی گرم در لیتر) کیتوزان به عنوان تیمار مورد استفاده قرار گرفت. صفات مورفولوژیکی مختلف، کلروفیل برگ و متابولیت­ های ثانویه اندازه گیری شد. تجزیه واریانس نشان داد که کیتوزان بر ارتفاع بوته، وزن تر، تعداد پیازچه، تعداد ریشه، کلروفیل a و b، کلروفیل کل، فنل‌ها، فلاونوئیدها و درصد باززایی تأثیر معنی‌داری داشت. بیشترین و کمترین وزن تر و تعداد ریشه به ترتیب از 200 پی پی ام کیتوزان و شاهد به دست آمد. همچنین نتایج نشان داد که ارتفاع بوته و تعداد پیازچه در تمامی غلظت‌های کیتوزان بیشتر از شاهد بود. بیشترین و کمترین میزان باززایی به ترتیب از کیتوزان 200 پی پی ام و شاهد به دست آمد. با این حال، بیشترین میزان فنل، فلاونوئیدها و کلروفیل از کیتوزان 200 پی پی ام حاصل شد. با توجه به نتایج مطالعه حاضر، کیتوزان به میزان 200 پی پی ام نسبت به سایر غلظت­ ها تأثیر مثبت بیشتری بر باززایی و تولید متابولیت­ های ثانویه Lilium regale  داشت.

Keywords [Persian]

  • باززایی
  • کشت درون شیشه ای
  • کیتوزان
  • گل سوسن
  • متابولیت های ثانویه
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