Shoot and root induction and growth of single nodes of Rosa damascena in different culture media

Document Type : Research Paper

Authors

Department of Biology, Shahid Bahonar University of Kerman, Kerman, Iran

Abstract

Rosa damascena is an important commercial crop in ornamental horticulture. Also, its essential oils and phenolic compounds are used in the medicinal, food, flavor, and perfume industries. The shoot and root regeneration of axillary single-node explants was studied on 16 and 24 different media, respectively. The combinations of 1, 1.5, 2, and 2.5 mg-1 of 6-benzylaminopurine (BAP) with 0.1 and 0.05 mgl-1 auxins [(2,4-D and 1-naphthaleneacetic acid (NAA)] and 0.1 mgl-1 gibberellic acid (GA3) were investigated for shoot induction. Shoot induction and proliferation were observed 16 and 38 days after planting, respectively. The highest shoot proliferation (100%) was obtained in the presence of 2.5 mgl-1 BAP and 0.05 mgl-1 of 2,4-D (medium 16). Root formation and growth of healthy shoots were studied on various combinations of indole-3 acetic acid (IAA) (0, 0.05, and 0.1 mgl-1) with full-strength and half-strength MS media in the solid and liquid culture media (12 media). In the optimal medium, root initiation and growth occurred 125 and 138 days after culturing, respectively. The successful rooting process occurred just in two media with rooting frequency of 31 and 53% respectively. Rooting frequency was higher in the half-strength MS liquid media than in the other media. 

Keywords

Article Title [Persian]

بنیان‌گذاری و رشد شاخساره و ریشه از تک‌گره‌های گل محمدی (Rosa damascene) در محیط کشت‌های مختلف

Authors [Persian]

  • فرحنده رضانژاد
  • مسلم آباریان
  • سمیه عبدی راد
گروه زیست شناسی، دانشگاه شهید باهنر کرمان، کرمان
Abstract [Persian]

گل محمدی (Rosa damascene) از گیاهان مهم تجاری و زینتی در باغبانی است. همچنین، از روغن‌های اساسی و ترکیبات فنلی آن در صنایع دارویی، غذایی، عطر سازی و نیز به عنوان طعم دهنده استفاده می‌شود. باززایی شاخساره و ریشه از ریزنمونه‌های تک‌گره‌ها، به ترتیب در 16 و 24 محیط مختلف مورد بررسی قرار گرفت. ترکیب 1، 1.5، 2 و 2.5 میلی‌گرم در لیتر  BAP با 0.1 و 0.05 میلی‌گرم در لیتر 2, 4-D و NAA و 0.1 میلی‌گرم در لیتر GA3 برای القای شاخساره بررسی شد. تحریک شاخسارزایی و پرآوری (تکثیر) به ترتیب 16 و 38 روز پس از کاشت مشاهده شد. بالاترین پرآوری شاخساره (100 درصد) در 2.5 میلی‌گرم در لیتر BAP و 0.05 میلی‌گرم در لیتر 2, 4-D (محیط شماره 16) به دست آمد. تشکیل و رشد ریشه از شاخسارهای سالم، در سه غلظت‌ مختلف IAA، یعنی 0،  0.05 و 0.1 میلی گرم در لیتر، و نیز تحت محیط MS کامل و یک دوم MS در محیط کشت جامد و مایع (12 محیط) مورد مطالعه قرار گرفت. در محیط بهینه، بنیان‌گذاری و رشد ریشه به ترتیب 125 و 138 روز پس از کشت اتفاق افتاد. روند موفقیت‌آمیز ریشه‌زایی فقط در دو محیط به ترتیب با میزان ریشه‌زایی 32 و 53 درصد دیده شد. فراوانی ریشه‌زایی در محیط نیمه مایع MS  نسبت به محیط کشت‌های دیگر بالاتر بود.

Keywords [Persian]

  • پرآوری؛ تشکیل شاخساره؛ نرخ ریشه‌زایی؛ 2
  • 4-D؛ IAA

References

Anderson RG and Woods TA, 1999. An economic evaluation of single stem cut rose production. Acta Horticulturae 481: 629-634.
Cai Z, Jing X, Tian X, Jiang J, Liu F, and Wang X, 2015. Direct and indirect in vitro plant regeneration and the effect of brassinolide on callus differentiation of Populus euphratica Oliv. South African Journal of Botany 97: 143-148.
Davoudi Pahnekolayi M, Samiei L, Tehranifar A and Shoor M, 2015. The effect of medium and plant growth regulators on micropropagation of Dog rose (Rosa canina L.). Journal of Plant Molecular Breeding 3(1): 61-71.
 
De Klerk GJ, Ter Brugge J, Jasik J, and Marinova S, 1997. Choice of auxin for in vitro rooting of apple microcuttings. In: Altman A and Waisel Y (eds). Biology of Root Formation and Development. Pp. 111-116. Basic Life Sciences, vol. 65. Springer, Boston, USA.
 
Epstein E and Muller JL, 1993. Indol-3-butyric acid in plants: occurrence, synthesis, metabolism and transport. Physiologia Plantarum. 88: 382-389.
Gago P, Santiago JL, Boso S, Alonso-Villaverde V, and Martinez MC, 2009. Grapevine (Vitis vinifera L.): old varieties are reflected in works of art. Economic Botany 63(1): 67-77.
Gazaryan IG, Lagrimini LM, Ashby GA, and Thorneley RN, 1996. Mechanism of indole-3-acetic acid oxidation by plant peroxidases: anaerobic stopped-flow spectrophotometric studies on horseradish and tobacco peroxidases. Biochemical Journal 313(3): 841-847.
George EF and Sherrington PD, 1984. Plant propagation by tissue culture: handbook and directory of commercial laboratories. Exegetics Ltd., Eversley, Basingstoke, Hants, UK, 709 pp.
Ginova A, Tsvetkov I, and Kondakova V, 2012. Rosa damascene Mill. - an overview for evaluation of propagation methods. Bulgarian Journal of Agricultural Science 18(4): 545-556.
Gubis J, Lajchová Z, Faragó J, and Jureková Z, 2003. Effect of genotype and explant type on shoot regeneration in tomato (Lycopersicon esculentum) in vitro. Czech Journal of Genetics and Plant Breeding 39 (1): 9-14.
Hajian S and Khosh-Khui M, 2000. Investigation on sexual and asexual propagation methods of Damask rose (Rosa damascena Mill.). Iran Agricultural Research 19(1): 1-6.
Hobbie L, McGovern M, Hurwitz LR, Pierro A, Liu NY, Bandyopadhyay A, and Estelle M, 2000. The axr6 mutants of Arabidopsis thaliana define a gene involved in auxin response and early development. Development 127(1): 23-32.
Hudson TH, Dale EK, Davies Jr, FT, and Geneve RL, 2002. Plant Propagation Principles and Practices, 6th ed. Prentice Hall of India Private Limited, New Delhi, India, 770 pp.
Jabbarzadeh Z and Khosh-Khui M, 2005. Factors affecting tissue culture of Damask rose (Rosa damascena Mill.). Scientia Horticulturae 105(4): 475-482.
Khosh-Khui M, Honarvar M, and Javidnia K, 2009. The first report on in vitro culture of Musk rose. Acta Horticulturae 870: 213-218.
Khosh-Khui M and Sink KC, 1982. Micropropagation of new and old world rose species. Journal of Horticultural Science 57(3): 315-319.
Kim CK, Oh JY, Jee SO, and Chung JD, 2003. In vitro micropropagation of Rosa hybrid L. Plant Biotechnology 5(2): 115-119.
Kirichenko EB, Kuz-Mina TA, and Kataeva NV, 1991. Factors in optimizing the multiplication of ornamental and essential oil roses in vitro. Byullenten-Glavnogo-Botanicheskogo Sada 159: 61-67.
Kornova KM and Michailova J, 1994. Study of the in vitro rooting of Kazanlak oil-bearing rose (Rosa damascena Mill.), Journal of Essential Oil Research 6(5): 485-492.
Kovacheva N, Rusanov K, and Atanassov I, 2010. Industrial cultivation of oil bearing rose and rose oil production in Bulgaria during 21st century, directions and challenges. Biotechnology & Biotechnological Equipment 24(2): 1793-1798.
Kumar A, Sood A, Palni U, Gupta A, and Palni LM, 2001. Micropropagation of Rosa damascena Mill. from mature bushes using thidiazuron. The Journal of Horticultural Science and Biotechnology 76(1): 30-34.
Kwaśniewska E and Pawłowska B, 2017. Efficient in vitro propagation of historical roses for biodiversity conservation. Propagation of Ornamental Plants 17(1): 3-11.
Ma Y, Byrne DH, and Chen J, 1996. Propagation of rose species in vitro. In Vitro Cellular and Developmental Biology - Plant 32: 103-108.
Mahboubi M, 2016. Rosa damascena as holy ancient herb with novel applications. Journal of Traditional and Complementary Medicine 6(1): 10-16.
Mahmoudi Noodezh H, Moieni A, and Baghizadeh A, 2012. In vitro propagation of the Damask rose (Rosa damascena Mill.). In Vitro Cellular & Developmental Biology – Plant 48(5): 530-538.
Moe I, 1973. Propagation, growth and flowering of potted roses.Acta Horticultrae 31: 157-166.
Mor Y and Zieslin N 1987. Plant growth regulators in rose plants. In: Janik J (ed.). Horticultural Reviews, volume 9. Pp. 53-57. Van Nostrand Reinhold Company Pub., New York, USA.
Muiruri SN, Mweu CM, and Nyende AB, 2011. Micropropagation protocols using nodal explants of selected rose (Rosa hybrida) cultivars. African Journal of Horticultural Science 4: 60-65.
Murashige T and Skoog F, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15(3): 473-497.
Nasri F, Fadakar A, Koshesh Saba M, and Yousefi B, 2015. Study of indole butyric acid (IBA) effects on cutting rooting improving some of wild genotypes of Damask roses (Rosa damascena Mill.). Journal of Agricultural Sciences 60(3): 263-275.
Ngezahayo F and Liu B, 2014. Axillary bud proliferation approach for plant biodiversity conservation and restoration. International Journal of Biodiversity ID 727025.
Nikbakht A and Kafi MA, 2008. Study on the relationships between Iranian people and Damask Rose (Rosa damascena) and its therapeutic and healing properties. Acta Horticulturae 790: 251–254.
Nunes HS and Miguel MG, 2017. Rosa damascena essential oils: a brief review about chemical composition and biological properties. Trends in Phytochemical Research 1(3): 111-128.
Pati PK, Kaur N, Sharma M, and Ahuja PS, 2010. In vitro propagation of rose. In: Jain S and Ochatt S (eds.). Protocols for In Vitro Propagation of Ornamental Plants. Methods in Molecular Biology (Methods and Protocols), vol. 589. Pp. 163-176. Humana Press.
Pati PK, Rath SP, Sharma M, Sood A, and Ahuja PS, 2006. In vitro propagation of rose—a review. Biotechnology advances 24(1), 94-114.
Pati PK, Sharma M, Sood A, and Ahuja PS, 2004. Direct shoot regeneration from leaf explants of Rosa damascena Mill. In Vitro Cellular & Developmental Biology - Plant 40(2): 192-195.
Rashidi M, Saremi-Rad A, Froozesh P, and Ghasemo Omran VO, 2018. Effect of plant growth regulators on Fittonia verschaffeltii regeneration at in vitro conditions. Journal of Plant Physiology and Breeding 8(2): 59-68.
Rout GR, Debata BK and Das P, 1990. In vitro clonal multiplication of roses. Proceedings of the National Academy of Sciences, India 60: 311-318.
Scott TK, 1972. Auxin and roots. Annual Review of Plant Physiology 23: 235-258.
Štefančič M, Štampar F, and Osterc G, 2005. Influence of IAA and IBA on root development and quality of Prunus‘GiSelA 5’ leafy cuttings. HortScience 40(7): 2052-2055.
Tarinejad A and Amiri S, 2019. Influence of plant growth regulators, carbohydrate source and concentration on micropropagation and other physiological traits of grape (Vitis vinifera L. cv. Shahroudi) under in vitro conditions. Journal of Plant Physiology and Breeding 9(1): 75-82.
Tarrahi R and Rezanejad F, 2013. Callogenesis and production of anthocyanin and chlorophyll in callus cultures of vegetative and floral explants in Rosa gallica and Rosa hybrida (Rosaceae). Turkish Journal of Botany 37(6): 1145-1154.
Vijaya N, Satyanarayana G, Prakash J, and Pierik RLM, 1991. Effect of culture media and growth regulators on in vitro propagation of rose. Current Plant Science Biotechnology in Agriculture 12: 209-214.
Wang Y, Wang Y, Li K, Song X, and Chen J, 2016. Characterization and comparative expression profiling of browning response in Medinilla formosana after cutting. Frontiers in Plant Science 7: 1897.
Wojtania A and Matysiak B, 2018. In vitro propagation of Rosa ‘Konstancin’ (R. rugosa × R. beggeriana), a plant with high nutritional and pro-health value. Folia Horticulturae 30(2): 259-67.
Wulster G and Sacalis J, 1980. Effects of auxins and cytokinins on ethylene evolution and growth of rose callus tissue in sealed vessels. Horticultural Science 15: 736-737.
 
 
 
Anderson RG and Woods TA, 1999. An economic evaluation of single stem cut rose production. Acta Horticulturae 481: 629-634.
Cai Z, Jing X, Tian X, Jiang J, Liu F, and Wang X, 2015. Direct and indirect in vitro plant regeneration and the effect of brassinolide on callus differentiation of Populus euphratica Oliv. South African Journal of Botany 97: 143-148.
Davoudi Pahnekolayi M, Samiei L, Tehranifar A and Shoor M, 2015. The effect of medium and plant growth regulators on micropropagation of Dog rose (Rosa canina L.). Journal of Plant Molecular Breeding 3(1): 61-71.
 
De Klerk GJ, Ter Brugge J, Jasik J, and Marinova S, 1997. Choice of auxin for in vitro rooting of apple microcuttings. In: Altman A and Waisel Y (eds). Biology of Root Formation and Development. Pp. 111-116. Basic Life Sciences, vol. 65. Springer, Boston, USA.
 
Epstein E and Muller JL, 1993. Indol-3-butyric acid in plants: occurrence, synthesis, metabolism and transport. Physiologia Plantarum. 88: 382-389.
Gago P, Santiago JL, Boso S, Alonso-Villaverde V, and Martinez MC, 2009. Grapevine (Vitis vinifera L.): old varieties are reflected in works of art. Economic Botany 63(1): 67-77.
Gazaryan IG, Lagrimini LM, Ashby GA, and Thorneley RN, 1996. Mechanism of indole-3-acetic acid oxidation by plant peroxidases: anaerobic stopped-flow spectrophotometric studies on horseradish and tobacco peroxidases. Biochemical Journal 313(3): 841-847.
George EF and Sherrington PD, 1984. Plant propagation by tissue culture: handbook and directory of comerical laboratories. Exegetics Ltd., Eversley, Basingstoke, Hants, UK, 709 pp.
Ginova A, Tsvetkov I, and Kondakova V, 2012. Rosa damascene Mill. - an overview for evaluation of propagation methods. Bulgarian Journal of Agricultural Science 18(4): 545-556.
Gubis J, Lajchová Z, Faragó J, and Jureková Z, 2003. Effect of genotype and explant type on shoot regeneration in tomato (Lycopersicon esculentum) in vitro. Czech Journal of Genetics and Plant Breeding 39 (1): 9-14.
Hajian S and Khosh-Khui M, 2000. Investigation on sexual and asexual propagation methods of Damask rose (Rosa damascena Mill.). Iran Agricultural Research 19(1): 1-6.
Hobbie L, McGovern M, Hurwitz LR, Pierro A, Liu NY, Bandyopadhyay A, and Estelle M, 2000. The axr6 mutants of Arabidopsis thaliana define a gene involved in auxin response and early development. Development 127(1): 23-32.
Hudson TH, Dale EK, Davies Jr, FT, and Geneve RL, 2002. Plant Propagation Principles and Practices, 6th ed. Prentice Hall of India Private Limited, New Delhi, India, 770 pp.
Jabbarzadeh Z and Khosh-Khui M, 2005. Factors affecting tissue culture of Damask rose (Rosa damascena Mill.). Scientia Horticulturae 105(4): 475-482.
Khosh-Khui M, Honarvar M, and Javidnia K, 2009. The first report on in vitro culture of Musk rose. Acta Horticulturae 870: 213-218.
Khosh-Khui M and Sink KC, 1982. Micropropagation of new and old world rose species. Journal of Horticultural Science 57(3): 315-319.
Kim CK, Oh JY, Jee SO, and Chung JD, 2003. In vitro micropropagation of Rosa hybrid L. Plant Biotechnology 5(2): 115-119.
Kirichenko EB, Kuz-Mina TA, and Kataeva NV, 1991. Factors in optimizing the multiplication of ornamental and essential oil roses in vitro. Byullenten-Glavnogo-Botanicheskogo Sada 159: 61-67.
Kornova KM and Michailova J, 1994. Study of the in vitro rooting of Kazanlak oil-bearing rose (Rosa damascena Mill.), Journal of Essential Oil Research 6(5): 485-492.
Kovacheva N, Rusanov K, and Atanassov I, 2010. Industrial cultivation of oil bearing rose and rose oil production in Bulgaria during 21st century, directions and challenges. Biotechnology & Biotechnological Equipment 24(2): 1793-1798.
Kumar A, Sood A, Palni U, Gupta A, and Palni LM, 2001. Micropropagation of Rosa damascena Mill. from mature bushes using thidiazuron. The Journal of Horticultural Science and Biotechnology 76(1): 30-34.
Kwaśniewska E and Pawłowska B, 2017. Efficient in vitro propagation of historical roses for biodiversity conservation. Propagation of Ornamental Plants 17(1): 3-11.
Ma Y, Byrne DH, and Chen J, 1996. Propagation of rose species in vitro. In Vitro Cellular and Developmental Biology - Plant 32: 103-108.
Mahboubi M, 2016. Rosa damascena as holy ancient herb with novel applications. Journal of Traditional and Complementary Medicine 6(1): 10-16.
Mahmoudi Noodezh H, Moieni A, and Baghizadeh A, 2012. In vitro propagation of the Damask rose (Rosa damascena Mill.). In Vitro Cellular & Developmental Biology – Plant 48(5): 530-538.
Moe I, 1973. Propagation, growth and flowering of potted roses.Acta Horticultrae 31: 157-166.
Mor Y and Zieslin N 1987. Plant growth regulators in rose plants. In: Janik J (ed.). Horticultural Reviews, volume 9. Pp. 53-57. Van Nostrand Reinhold Company Pub., New York, USA.
Muiruri SN, Mweu CM, and Nyende AB, 2011. Micropropagation protocols using nodal explants of selected rose (Rosa hybrida) cultivars. African Journal of Horticultural Science 4: 60-65.
Murashige T and Skoog F, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15(3): 473-497.
Nasri F, Fadakar A, Koshesh Saba M, and Yousefi B, 2015. Study of indole butyric acid (IBA) effects on cutting rooting improving some of wild genotypes of Damask roses (Rosa damascena Mill.). Journal of Agricultural Sciences 60(3): 263-275.
Ngezahayo F and Liu B, 2014. Axillary bud proliferation approach for plant biodiversity conservation and restoration. International Journal of Biodiversity ID 727025.
Nikbakht A and Kafi MA, 2008. Study on the relationships between Iranian people and Damask Rose (Rosa damascena) and its therapeutic and healing properties. Acta Horticulturae 790: 251–254.
Nunes HS and Miguel MG, 2017. Rosa damascena essential oils: a brief review about chemical composition and biological properties. Trends in Phytochemical Research 1(3): 111-128.
Pati PK, Kaur N, Sharma M, and Ahuja PS, 2010. In vitro propagation of rose. In: Jain S and Ochatt S (eds.). Protocols for In Vitro Propagation of Ornamental Plants. Methods in Molecular Biology (Methods and Protocols), vol. 589. Pp. 163-176. Humana Press.
Pati PK, Rath SP, Sharma M, Sood A, and Ahuja PS, 2006. In vitro propagation of rose—a review. Biotechnology advances 24(1), 94-114.
Pati PK, Sharma M, Sood A, and Ahuja PS, 2004. Direct shoot regeneration from leaf explants of Rosa damascena Mill. In Vitro Cellular & Developmental Biology - Plant 40(2): 192-195.
Rashidi M, Saremi-Rad A, Froozesh P, and Ghasemo Omran VO, 2018. Effect of plant growth regulators on Fittonia verschaffeltii regeneration at in vitro conditions. Journal of Plant Physiology and Breeding 8(2): 59-68.
Rout GR, Debata BK and Das P, 1990. In vitro clonal multiplication of roses. Proceedings of the National Academy of Sciences, India 60: 311-318.
Scott TK, 1972. Auxin and roots. Annual Review of Plant Physiology 23: 235-258.
Štefančič M, Štampar F, and Osterc G, 2005. Influence of IAA and IBA on root development and quality of Prunus‘GiSelA 5’ leafy cuttings. HortScience 40(7): 2052-2055.
Tarinejad A and Amiri S, 2019. Influence of plant growth regulators, carbohydrate source and concentration on micropropagation and other physiological traits of grape (Vitis vinifera L. cv. Shahroudi) under in vitro conditions. Journal of Plant Physiology and Breeding 9(1): 75-82.
Tarrahi R and Rezanejad F, 2013. Callogenesis and production of anthocyanin and chlorophyll in callus cultures of vegetative and floral explants in Rosa gallica and Rosa hybrida (Rosaceae). Turkish Journal of Botany 37(6): 1145-1154.
Vijaya N, Satyanarayana G, Prakash J, and Pierik RLM, 1991. Effect of culture media and growth regulators on in vitro propagation of rose. Current Plant Science Biotechnology in Agriculture 12: 209-214.
Wang Y, Wang Y, Li K, Song X, and Chen J, 2016. Characterization and comparative expression profiling of browning response in Medinilla formosana after cutting. Frontiers in Plant Science 7: 1897.
Wojtania A and Matysiak B, 2018. In vitro propagation of Rosa ‘Konstancin’ (R. rugosa × R. beggeriana), a plant with high nutritional and pro-health value. Folia Horticulturae 30(2): 259-67.
Wulster G and Sacalis J, 1980. Effects of auxins and cytokinins on ethylene evolution and growth of rose callus tissue in sealed vessels. Horticultural Science 15: 736-737.
Journal of Plant Physiology and Breeding
Volume 11, Issue 2
December 2021
Pages 97-108

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