Salicylic acid: an effective growth regulator for mitigating salt toxicity in plants

Document Type : Review Paper


Department of Plant Ecophysiology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.


Salinity is a harmful environmental factor threatening plant growth and productivity through ionic and oxidative stresses. These detrimental effects of salinity could be modulated by some plant growth regulators. Salicylic acid (SA) as a phenolic molecule regulates growth and development and also induces crucial defense mechanisms in plants under salinity. This growth regulator can also improve some physiological and biochemical processes of salt-stressed plants such as reducing Na+ influx to the root cells and increasing essential nutrients uptake. Application of SA can also help plants to accumulate the toxic Na+ in vacuoles through enhancing the activities of H+-pum- stressed plants. Some reports indicate that salicylic acid counteracts salt-induced water deficit by elevating plant osmolytes including soluble sugars, proline, and glycine betaine. These essential roles as well as the effect of SA in the augmentation of chlorophyll and photosynthetic activities can potentially improve plant growth and productivity under saline conditions. The possible cross-talks of salicylic acid with other growth regulators are also important for promoting salt tolerance and the performance of plants under stressful conditions. 


Article Title [فارسی]

سالیسیلیک اسید: یک تنظیم کننده رشد مؤثر برای کاهش سمیت نمک در گیاهان

Authors [فارسی]

  • سهیلا عبدلی
  • کاظم قاسمی گلعذانی
گروه اکوفیزیولوژی، دانشکده کشاورزی، دانشگاه تبریز، تبریز.
Abstract [فارسی]

شوری یک عامل محیطی مضر است که از طریق تنش­ های یونی و اکسیداتیو رشد و تولید گیاه را تهدید می­ کند. این اثرات زیان­بار شوری می­ تواند توسط برخی تنظیم کننده­ های رشد گیاهی تعدیل گردد. سالیسیلیک اسید (SA) به عنوان یک مولکول فنلی رشد و نمو را تنظیم کرده و مکانیسم ­های دفاعی حیاتی گیاهان را تحت تنش شوری القا می­ کند. این تنظیم کننده رشد می ­تواند برخی فرایند­های فیزیولوژیکی و بیوشیمیایی گیاهان تحت تنش شوری مانند کاهش ورود سدیم به سلول­ های ریشه و افزایش جذب عناصر غذایی ضروری را بهبود بخشد. کاربرد SA همچنین می­ تواند از طریق افزایش فعالیت پمپ­ های پروتونی به ذخیره سدیم سمی در واکوئل گیاهان کمک کند. این هورمون گیاهی ظرفیت آنتی­اکسیدانی (آنزیمی و غیر آنزیمی) گیاهان تحت تنش شوری را به طور قابل توجهی افزایش می­ دهد. برخی گزارش ­ها نمایانگر آن است که سالیسیلیک اسید از طریق افزایش محتوای اسمولیت ­ها از جمله قند­های محلول، پرولین و گلایسین بتائین با کمبود آب ناشی از شوری مقابله می­ کند. این نقش­ های اساسی و همچنین اثرات SA در افزایش کلروفیل و فعالیت­ های فتوسنتزی می­ تواند قابلیت رشد و تولید گیاهان را تحت تنش شوری بهبود دهد. روابط احتمالی سالیسیلیک اسید با سایر تنظیم کننده ­های رشد هم در بهبود تحمل شوری و عملکرد گیاهان در شرایط تنش ­زا اهمیت دارند.

Keywords [فارسی]

  • آنتی اکسیدان
  • تحمل شوری
  • سالیسیلیک اسید
  • فعالیت فتوسنتزی
  • کلروفیل
Abdoli S, Ghassemi-Golezani K, and Alizadeh-Salteh S, 2020. Responses of ajowan (Trachyspermum ammi L.) to exogenous salicylic acid and iron-oxide nanoparticles under salt stress. Environmental Science and Pollution Research 27: 36939-36953.
Agtuca B, Rieger E, Hilger K, Song L, Robert CAM, and Erb M, 2014. Carbon-11 reveals opposing roles of auxin and salicylic acid in regulating leaf physiology, leaf metabolism, and resource allocation patterns that impact root growth in Zea mays. Journal of Plant Growth Regulation 33: 328-339.
Alonso-Ramirez A, Rodriguez D, Reyes D, Jimenez JA, Nicolas G, Lopez-Climent M, GomezCadenas A, and Nicolas C, 2009. Evidence for a role of gibberellins in salicylic acid-modulated early plant responses to abiotic stress in Arabidopsis seeds. Plant Physiology150: 1335-1344.
Alvarez I, Tomaro ML, and Benavides MP, 2003. Changes in polyamines, proline, and ethylene in sunflower calluses treated with NaCl. Plant Cell, Tissue and Organ Culture 74: 51-59.
Apel K and Hirt H, 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology 55: 373-399.
Apse MP, Aharon GS, Snedden WA, and Blumwald E, 1999. Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiporter in Arabidopsis. Science 285: 1256-1258.
Asensi-Fabado MA and Munné-Bosch S, 2011. The aba3-1 mutant of Arabidopsis thaliana withstands moderate doses of salt stress by modulating leaf growth and salicylic acid levels. Journal of Plant Growth Regulation 30: 456-466.
Bassil E and Blumwald E, 2014. The ins and outs of intracellular ion homeostasis: NHX-type cation/H+ transporters. Current Opinion in Plant Biology 22: 1-6.
Batista VCV, Pereira IMC, Paula-Marinho SO, Canuto KM, Pereira RCA, Rodrigues THS, Daloso DM, Gomes-Filho E, and Carvalho HH, 2019. Salicylic acid modulates primary and volatile metabolites to alleviate salt stress-induced photosynthesis impairment on medicinal plant Egletes viscosa. Environmental and Experimental Botany 167:  103870.
Bouchereau A, Aziz A, Larher F, and Martin-Tanguy J, 1999. Polyamines and environmental challenges: recent development. Plant Science 140: 103-125.
Chao YY, Chen CY, Huang WD, and Ching CH, 2010. Salicylic acid mediated hydrogen peroxide accumulation and protection against Cd toxicity in rice leaves. Plant and Soil 329: 327-337.
Chaparzadeh N and Hosseinzad-Behboud E, 2015. Evidence for enhancement of salinity induced oxidative damages by salicylic acid in radish (Raphanus sativus L.). Journal of Plant Physiology and Breeding 5: 23-33.
Chattopadhayay MK, Tiwari BS, Chattopadhyay G, Bose A, Sengupta DN, and Ghosh B, 2002. Protective role of exogenous polyamines on salinity-stressed rice (Oryza sativa) plants. Physiologia Plantarum 116: 192-199.
Csiszár J, Horváth E, Váry Z, Gallé Á, Bela K, Brunner S, and Tari I, 2014. Glutathione transferase supergene family in tomato: salt stress-regulated expression of representative genes from distinct GST classes in plants primed with salicylic acid. Plant Physiology and Biochemistry 78: 15-26.
Das S, Bose A, and Ghosh B, 1995. Effect of salt stress on polyamine metabolism in Brassica campestris. Phytochemistry 39: 283-285.
Ding Y, Zhao J, Nie Y, Fan B, Wu S, Zhang Y, Sheng J, Shen L, Zhao R, and Tang X, 2016. Salicylic acid-induced chilling- and oxidative-stress tolerance in relation to gibberellin homeostasis, C-repeat/dehydration- responsive element binding factor pathway, and antioxidant enzyme systems in cold-stored tomato fruit. Journal of Agricultural and Food Chemistry 64: 8200-8206.
Egamberdieva D, 2009. Alleviation of salt stress by plant growth regulators and IAA producing bacteria in wheat. Acta Physiologiae Plantarum, 31: 861-864.
El-Esawi MA, Elansary HO, El-Shanhorey NA, Abdel-Hamid AME, Ali HM, and Elshikh MS, 2017. Salicylic acid-regulated antioxidant mechanisms and gene expression enhance rosemary performance under saline conditions. Frontiers in Physiology 8: 716.
El-Tayeb MA, 2005. Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regulation 45: 215-224.
Elyasi R, Majdi M, Bahramnejad B, and Mirzaghaderi G, 2016. Spatial modulation and abiotic elicitors responses of the biosynthesis related genes of mono/triterpenes in black cumin (Nigella sativa). Industrial Crops and Products 79: 240-247.
Fahad S, Hussain S, Bano A, Saud S, Hassan S, Shan D, Khan FA, Khan F, Chen YT, Wu C, Tabassum MA, Chun MX, Afzal M, Jan A, Jan MT, and Huang JL, 2015. Potential role of phytohormones and plant growth-promoting rhizobacteria in abiotic stresses: consequences for changing environment. Environmental Science and Pollution Research 22: 4907-4921.
Farhadi N and Ghassemi-Golezani K, 2020. Physiological changes of Mentha pulegium in response to exogenous salicylic acid under salinity. Scientia Horticulturae 267: 109325.
Farhangi-Abriz S and Ghassemi-Golezani K, 2018. How can salicylic acid and jasmonic acid mitigate salt toxicity in soybean plants? Ecotoxicology and Environmental Safety 147: 1010-1016.
Farhangi-Abriz S, Tavasolee A, Ghassemi-Golezani, K, Torabian S,Monirifar H, and Asadi Rahmani H, 2020. Growth-promoting bacteria and natural regulators mitigate salt toxicity and improve rapeseed plant performance. Protoplasma 257: 1035-1047.
Fathi S, Kharazmi M, and Najafian S, 2019. Effects of salicylic acid foliar application on morpho-physiological traits of purslane (Portulaca olaracea L.) under salinity stress conditions. Journal of Plant Physiology and Breeding 9: 1-9.
Fayez KA and Bazaid SA, 2014. Improving drought and salinity tolerance in barley by application of salicylic acid and potassium nitrate. Journal of the Saudi Society of Agricultural Sciences 13: 45-55.
Garcion C, Lohmann A, Lamodière E, Catinot J, Buchala A, Doermann P, and Métraux JP, 2008.
Characterization and biological function of the ISOCHORISMATE SYNTHASE2 gene of Arabidopsis. Plant Physiology 147: 1279-1287.
Ghasemi Pirbalouti A, Rahmani Samani M, Hashemi M, and Zeinali H, 2014. Salicylic acid affects growth, essential oil and chemical compositions of thyme (Thymus daenensis Celak.) under reduced irrigation. Plant Growth Regulation 72: 289-301. 
Ghassemi-Golezani K and Abdoli S, 2021. Improving ATPase and PPase activities, nutrient uptake and growth of salt stressed ajowan plants by salicylic acid and iron-oxide nanoparticles. Plant Cell Reports 40: 559-573.
Ghassemi-Golezani K and Farhangi-Abriz S, 2018. Foliar sprays of salicylic acid and jasmonic acid stimulate H+-ATPase activity of tonoplast, nutrient uptake and salt tolerance of soybean. Ecotoxicology and Environmental Safety 166: 18-25.
Ghassemi-Golezani K, Farhangi-Abriz S, and Abdoli S, 2021. How can biochar-based metal oxide nanocomposites counter salt toxicity in plants? Environmental Geochemistry and Health. 43: 2007-2023.
Ghassemi-Golezani K, Hassanzadeh N, Shakiba MR, and Esmaeilpour B, 2020a. Exogenous salicylic acid and 24-epi-brassinolide improve antioxidant capacity and secondary metabolites of Brassica nigra. Biocatalysis and Agricultural Biotechnology 26: 101636.
Ghassemi Golezani K, Hosseinzadeh Mahootchi A, and Farhangi Abriz S, 2020b.Chlorophyll a fluorescence of safflower affected by salt stress and hormonal treatments. SN Applied Sciences 2: 1306.
Ghassemi-Golezani K and Nikpour-Rashidabad N, 2017. Seed pretreatment and salt tolerance of dill: osmolyte accumulation, antioxidant enzymes activities and essence production. Biocatalysis and Agricultural Biotechnology 12: 30-35.Gill SS and Tuteja N, 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48: 909-930.
Gunes A, Inal A, Alpaslan M, Eraslan F, Guneri Bagci E, and Cicek N, 2007. Salicylic acid induced changes on some physiological parameters symptomatic for oxidative stress and mineral nutrition in maize (Zea mays L.) grown under salinity. Journal of Plant Physiology 164: 728-736.
Horváth E, Szalai G, and Janda T, 2007. Induction of abiotic stress tolerance by salicylic acid signaling. Journal of Plant Growth Regulation 26: 290-300.
Iglesias MJ, Terrile MC, and Casalongue CA, 2011. Auxin and salicylic acid signalings counteract during the adaptive response to stress. Plant Signaling and Behavior 6: 452-454.
Ismail A, Takeda S, and Nick P, 2014. Life and death under salt stress: same players, different timing? Journal of Experimental Botany 65: 2963-2979.
Jayakannan M, Bose J, Babourina O, Rengel Z, and Shabala S, 2013. Salicylic acid improves salinity tolerance in Arabidopsis by restoring membrane potential and preventing salt-induced K+ loss via a GORK channel. Journal of Experimental Botany 64: 2255-2268.
Jayakannan M, Bose J, Babourina O, Rengel Z, and Shabala S, 2015. Salicylic acid in plant salinity stress signalling and tolerance. Plant Growth Regulation 76: 25-40.
Juan ME, Gonzalez-Pons E, Munuera T, Ballester J, Rodriguez-Gil JE, and Planas JM, 2005. Trans resveratrol, a natural antioxidant from grapes, increases sperm output in healthy rats. The Journal of Nutrition 135: 757-760.
Kamran M, Xie K, Sun J, Wang D, Shi C, Lu Y, Gu W, and Xu P, 2020. Modulation of growth performance and coordinated induction of ascorbate glutathione and methylglyoxal detoxification systems by salicylic acid mitigates salt toxicity in choysum (Brassica parachinensis L.). Ecotoxicology and Environmental Safety 188: 109877.
Keisham M, Mukherjee S, and Bhatla SC, 2018. Mechanisms of sodium transport in plants-progresses and challenges. International Journal of Molecular Sciences 19: 647.
Khan MIR, Asgher M, and Khan NA, 2014. Alleviation of salt-induced photosynthesis and growth inhibition by salicylic acid involves glycine betaine and ethylene in mung bean (Vigna radiataL.). Plant Physiology and Biochemistry 80: 67-74.
Khoshbakht D and Asgharei MR, 2015. Influence of foliar-applied salicylic acid on growth, gas-exchange characteristics, and chlorophyll fluorescence in citrus under saline conditions. Photosynthetica 53: 410-418.
Kumar S, Kalita A, Srivastava R, and Sahoo L, 2017. Co-expression of Arabidopsis NHX1 and bar improves the tolerance to salinity, oxidative stress, and herbicide in transgenic mung bean. Frontiers in Plant Science 8: 1896.  
Li G, Peng X, Wei L, and Kang G, 2013. Salicylic acid increases the contents of glutathione and ascorbate and temporally regulates the related gene expression in salt-stressed wheat seedlings. Gene 529: 321-325.
Métraux JP, 2002. Recent breakthroughs in the study of salicylic acid biosynthesis. Trends in Plant Science 7: 332-334.
Mirzajani Z, Hadavi E, and Kashi A, 2015. Changes in the essential oil content and selected traits of sweet basil (Ocimum basilicum L.) as induced by foliar sprays of citric acid and salicylic acid. Industrial Crops and Products 76: 269–274.
Miura K, Okamoto H, Okuma E, Shiba H, Kamada H, Hasegawa PM, and Murata Y, 2013. SIZ1 deficiency causes reduced stomatal aperture and enhanced drought tolerance via controlling salicylic acid induced accumulation of reactive oxygen species in Arabidopsis. The Plant Journal 49: 79-90.
Miura K, Sato A, Ohta M, and Furukawa J, 2011. Increased tolerance to salt stress in the phosphate-accumulating Arabidopsis mutants siz1 and pho2. Planta 234: 1191-1199.
Miura K and Tada Y, 2014. Regulation of water, salinity, and cold stress responses by salicylic acid. Frontiers in Plant Science 5: 4.
Mustafa NR, Kim HK, Choi YH, Erkelens C, Lefeber AW, Spijksma G, van der Heijden R, and Verpoorte R, 2009. Biosynthesis of salicylic acid in fungus elicited Catharanthus roseus cells. Phytochemistry 70: 532-539.
Nazar R, Iqbal N, Syeed S, and Khan NA, 2011. Salicylic acid alleviates decreases in photosynthesis under salt stress by enhancing nitrogen and sulfur assimilation and antioxidant metabolism differentially in two mung bean cultivars. Journal of Plant Physiology 168: 807-815.
Nazar R, Umar S, and Khan NA, 2015a. Exogenous salicylic acid improves photosynthesis and growth through increase in ascorbate-glutathione metabolism and S assimilation in mustard under salt stress. Plant Signaling and Behavior 10: e1003751.
Nazar R, Umar S, Khan NA, and Sareer O, 2015b. Salicylic acid supplementation improves photosynthesis and growth in mustard through changes in proline accumulation and ethylene formation under drought stress. South African Journal of Botany 98: 84-94.
Palma F, López-Gómez M, Tejera NA, and Lluch C, 2013. Salicylic acid improves the salinity tolerance of Medicago sativa in symbiosis with Sinorhizobium meliloti by preventing nitrogen fixation inhibition. Plant Science 208: 75-82.
Park JE, Park JY, Kim YS, Staswick PE, Jeon J, Yun J, Kim SY, Kim J, Lee YH, and Park CM, 2007. GH3-mediated auxin homeostasis links growth regulation with stress adaptation response in Arabidopsis. Journal of Biological Chemistry 282: 10036-10046.
Pirasteh-Anosheh H and Emam Y, 2018. Modulation of oxidative damage due to salt stress using salicylic acid in Hordeum vulgare. Archives of Agronomy and Soil Science 64: 1268-1277.
Pirasteh-Anosheh H, Emam Y, Hashemi SE, and Gaur A, 2021. Role of chlormequat chloride and salicylic acid in improving cereals performance under saline condition. In:  Sareen S, Sharma P, Singh C, Jasrotia P, Singh GP, and Sarial AK (Eds). Improving Cereal Productivity Through Climate Smart Practices. Pp. 145-158. Elsevier.
Pirasteh-Anosheh H, Emam Y, Rousta MJ, and Ashraf M, 2017. Salicylic acid induced salinity tolerance through manipulation of ion distribution rather than ion accumulation. Journal of Plant Growth Regulation 36: 227-239.
Pirasteh-Anosheh H, Emam Y, and Sepaskhah AR, 2015. Improving barley performance by proper foliar applied salicylic-acid under saline conditions. International Journal of Plant Production 9: 467-486.
Popova LP, Maslenkova LT, Yordanova RY, Ivanova AP, Krantev AP, Szalai G, and Janda T, 2009. Exogenous treatment with salicylic acid attenuates cadmium toxicity in pea seedlings. Plant Physiology and Biochemistry 47: 224-231.
Rady MM, Talaat NB, Abdelhamid MT, Shawky BT, and Desoky ESM, 2019. Maize (Zea mays L.) grains extract mitigates the deleterious effects of salt stress on common bean (Phaseolus vulgaris L.) growth and physiology. Journal of Horticultural Science and Biotechnology 94: 777-789.
Rai VK, Sharma SS, and Sharma S, 1986. Reversal of ABA induced stomatal closure by phenolic compounds. Journal of Experimental Botany 37: 129-134.
Rekhter D, Lüdke D, Ding Y, Feussner K, Zienkiewicz K, Lipka V, Wiermer M, Zhang Y, and Feussner I, 2019. Isochorismate-derived biosynthesis of the plant stress hormone salicylic acid. Science 365: 498-502.
Shakirova FM, Sakhabutdinova AR, Bezrukova MV, Fatkhutdinova RA, and Fatkhutdinova DR, 2003. Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Science 164: 317-322.
Sharma A, Sidhu GPS, Araniti F, Bali AS, Shahzad B, Tripathi DK, Brestic M, Skalicky M, and Landi M. 2020. The role of salicylic acid in plants exposed to heavy metals. Molecules 25: 540.
Shashi S, Sharma SS, and Rai VK, 1986. Reversal by phenolic compounds of abscisic acid induced inhibition of in vitro activity of amylase from seeds of Triticum aestivum L. New Phytologist 103: 293-297.
Sheteiwy MS, An J, Yin M, Jia X, Guan Y, He F, and Hu J, 2019. Cold plasma treatment and exogenous salicylic acid priming enhances salinity tolerance of Oryza sativa seedlings. Protoplasma 256: 79-99.
Singh PK and Gautam S, 2013. Role of salicylic acid on physiological and biochemical mechanism of salinity stress tolerance in plants. Acta Physiologiae Plantarum 35: 2345-2353.
Sorkheha K, Shiran B, Khodambashi M, Rouhi V, Mosavei S, and Sofo A, 2012. Exogenous proline alleviates the effects of H2O2-induced oxidative stress in wild almond species. Russian Journal of Plant Physiology 59: 788-798.
Szalai G, Pál M, Arendas T, and Janda T, 2016. Priming of seed with salicylic acid increases grain yield and modifies polyamine levels in maize. Cereal Research Communications 44: 537-548.
Szepesi Á, Csiszár J, Gémes K, Horváth E, Horváth F, and Simon ML, 2009. Salicylic acid improves acclimation to salt stress by stimulating abscisic aldehyde oxidase activity and abscisic acid accumulation, and increases Na+ content in leaves without toxicity symptoms in Solanum lycopersicum L. Journal of Plant Physiology 166: 914-925.
Tirani MM, Nasibi F, and Kalantari KM, 2013. Interaction of salicylic acid and ethylene and their effects on some physiological and biochemical parameters in canola plants (Brassica napus L.). Photosynthetica 51: 411-418.
Tounekti T, Hernández I, and Munné-Bosch S, 2013. Salicylic acid biosynthesis and role in modulating terpenoid and flavonoid metabolism in plant responses to abiotic stress. In: Hayat S, Ahmad A, and Alyemeni MN (Eds). Salicylic Acid. Pp. 141–162. Springer, Netherlands.
Ward JM, Maser P, and Schroeder JI, 2009. Plant ion channels: gene families, physiology, and functional genomics analyses. Annual Review of Physiology 71: 59-82.
Wildermuth MC, Dewdney J, Wu G, and Ausubel FM, 2001. Isochorismate synthase is required to synthesize salicylic acid for plant defense. Nature 414: 562-565.
Wu QS, Zou YN, Liu W, Ye XF, Zai HF, and Zhao LJ, 2010. Alleviation of salt stress in citrus seedlings inoculated with mycorrhiza: changes in leaf antioxidant defense systems. Plant, Soil and Environment 56: 470-475.
Yamaguchi T, Hamamoto S, and Uozumi N, 2013. Sodium transport system in plant cells. Frontiers in Plant Science 4: 410.
Yamaguchi M and Sharp RE, 2010. Complexity and coordination of root growth at low water potentials: recent advances from transcriptomic and proteomic analyses. Plant, Cell and Environment 33:590-603.
Yasuda M, Ishikawa A, and Jikumaru Y, 2008. Antagonistic interaction between systemic acquired resistance and the abscisic acid-mediated abiotic stress response in Arabidopsis. Plant Cell 20: 1678-1692.
Zhang HX and Blumwald E, 2001. Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit. Nature Biotechnology 19: 765-768.
Zhang HX, Hodson JN, Williams JP, and Blumwald E, 2001. Engineering salt-tolerant Brassica plants: characterization of yield and seed oil quality in transgenic plants with increased vacuolar sodium accumulation. Proceedings of the National Academy of Sciences of the United States of America 98: 12832-12836.
Zhu JK, 2003. Regulation of ion homeostasis under salt stress. Current Opinion in Plant Biology 6: 441-445.
Zörb C, Noll A, Karl S, Leib K, Yan F, and Schubert S, 2005. Molecular characterization of Na+/H+ antiporters (ZmNHX) of maize (Zea mays L.) and their expression under salt stress. Journal of Plant Physiology 162: 55-66.