Transferability of wheat SSR markers for determination of genetic diversity and relationships of barley varieties

Document Type : Research Paper


1 Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran.

2 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

3 ِDepartment of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

4 Dryland Agriculture Research Institute of Iran, Maragheh, Iran.



Simple sequence repeat (SSR) markers are simple PCR-based co-dominant markers, which are highly polymorphic and informative due to the number and frequency of alleles, and thus they are most utilized among the molecular markers. However, the development of SSR markers is costly and time-consuming. Cross-species transferability of SSRs allows the SSRs isolated from one species to apply on a closely related species, which increases the utility of previously isolated SSRs. This study demonstrated the cross-species transferability of 196 SSR primer pairs of wheat in genetic diversity analysis of 40 varieties of barley (Hordeum vulgare L.). Of the 196 SSR primer pairs assayed, 59 (30.1%) showed transferability. Of the 59 primer pairs, 21 pairs were polymorphic with the polymorphism information content ranging from 0.19 to 0.70. The number of alleles detected at each locus ranged from one to seven with an average of 3.57. Cluster analysis using the Minimum Evolution algorithm and the coefficient of Number of Differences assigned the genotypes into five groups.


Article Title [فارسی]

انتقال پذیری نشانگر های SSR گندم برای بررسی تنوع و روابط ژنتیکی ارقام جو

Authors [فارسی]

  • هما زلقی 1
  • سید ابوالقاسم محمدی 2
  • محمد مقدم 3
  • بهزاد صادق زاده 4
1 گروه به نژادی و بیوتکنولوژی گیاهی، دانشگاه تبریز، تبریز.
2 گروه به نژادی و بیوتکنولوژی گیاهی دانشگاه تبریز، تبریز.
3 گروه به نژادی و بیوتکنولوژی گیاهی دانشگاه تبریز، تبریز.
4 موسسه تحقیقات کشاورزی دیم کشور، مراغه.
Abstract [فارسی]

نشانگرهای SSR نشانگرهای هم بارز مبتنی بر  PCR هستند که به دلیل ماهیت چندآللی و فراوانی بالای آللی، دارای چندشکلی بالا و حاوی اطلاعات زیادی می­ باشند و بنابراین کاربرد فروانی در میان نشانگرهای مولکولی دارند. در عین حال، توسعه نشانگرهای SSR بسیار پرهزینه و زمان­ بر می­ باشد. انتقال پذیری بین گونه­ ای نشانگرهای  SSRامکان استفاده از نشانگرهای SSR طراحی شده برای یک گونه را در گونه­ های نزدیک فراهم می­ کند و بنابراین فراوانی استفاده از نشانگرهایی را که قبلا طراحی شده ­اند، افزایش می­ دهد. در این مطالعه انتقال­ پذیری و چندشکلی 169 نشانگر SSR که برای گندم (Triticum aestivum L.) طراحی شده بودند، در 40 ژنوتیپ جو (Hordeum vulgare L.)  بررسی شد. از 169 نشانگر، 59 (30.1%) آغازگر انتقال­ پذیری نشان دادند که از این 59 جفت آغازگر، 21 جفت چندشکل بودند (با متوسط ارزش PIC 0.19 تا 0.68). تعداد الل مشاهده شده در هر جایگاه بین 1 تا 7 الل و با متوسط 3.57 متغیر بود. تجزیه خوشه ­ای با استفاده از الگوریتم Minimum Evaluation و ضریب فاصله Number of Difference ژنوتیپ­ ها را در 5 گروه مجزا قرار داد.

Keywords [فارسی]

  • انتقال پذیری
  • جو
  • گندم
  • SSR
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