Antioxidant properties of two alfalfa (Medicago sativa L.) ecotypes in response to sodium chloride salinity stress

Document Type : Research Paper

Authors

1 Department of Biology, College of Sciences, Yadegar-e- Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran.

2 Department of Biology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran.

3 3Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Abstract

Biochemical and physiological responses of alfalfa under salinity stress were comparatively studied in a factorial experiment based on randomized complete block design by using Yazdi as the tolerant and Diabolourde as the sensitive ecotypes. Salt levels of 100, 150 and 200 mM were prepared by adding sodium chloride to the Hoagland half-strength culture medium. Total phenolics content, polyphenol oxidase (PPO), β-glucosidase and antiradical activities of leave′s extract, stomata properties and chlorophyll fluorescence parameters of the leaves including Fv/Fm and Fv/Fo were measured in response to salinity stress. Stomata characters were reduced in both ecotypes but chlorophyll fluorescence parameters only declined in Diabolourde but not in the Yazdi ecotype.  The PPO and β-glucosidase activities increased in both ecotypes. The phenolics content and antiradical activities increased in the Yazdi ecotype at all salt levels but those of Diabolurde increased only at the higher salinity levels. Our observations indicated that the Yazdi ecotype manipulated biochemical and physiological responses more efficiently to alleviate the reduction of growth parameters under salinity.  

Keywords

Article Title [Persian]

ویژگی های آنتی اکسیدانی دو اکوتیپ یونجه (Medicago sativa L.) در پاسخ به تنش شوری کلرید سدیم

Authors [Persian]

  • سید افشین حسینی بلداجی 1
  • بابک باباخانی 2
  • رضا حسن-ساجدی 3
  • مهدیه هوشنی 2
1 گروه زیست شناسی، دانشکده علوم پایه، واحد یادگار امام خمینی (ره) شهر ری، دانشگاه آزاد اسلامی، شهر ری
2 گروه زیست شناسی، دانشکده علوم زیستی، واحد تنکابن دانشگاه آزاد اسلامی، تنکابن.
3 گروه بیوشیمی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران.
Abstract [Persian]

پاسخ ­های بیوشیمیایی و فیزیولوژبک یونجه در یک آزمایش فاکتوریل بر پایه بلوک ­های کامل تصادفی با استفاده از اکوتیپ­ های یزدی و دیابلورده به ترتیب به عنوان اکوتیپ­ های مقاوم و حساس بررسی شد. سطوح شوری 100، 150 و 200 میلی مولار با اضافه کردن نمک کلرید سدیم به محیط کشت نیم-قدرت هوگلند تهیه شد. اثر تنش شوری بر ویژگی­ های بیوشیمایی و فیزیولوژیک شامل محتوی فنلی کل، فعالیت آنزیم­ های پلی فنل اکسیداز و بتاگلوکوزیداز در عصاره برگی، رفتار روزنه­ ای و پارامترهای فلورسانس کلروفیل (Fv/Fm وFv/F0 ) تعیین شد. نتایج نشان داد که ویژگی­ های روزنه ­ای در هر دو اکوتیپ کاهش یافت ولی فلورسانس کلروفیل فقط در دیابلورده کاهش نشان داد و در اکوتیپ یزدی تغییری مشاهده نشد. میزان کاهش این پارامترها بین دو اکوتیپ متفاوت بود. فعالیت آنزیم­ های پلی فنل اکسیداز و بتاگلوکوزیداز در هر دو اکوتیپ افزایش یافت. محتوی فنلی کل و فعالیت آنتی اکیسیدانی در تمامی تیمارها در اکوتیپ یزدی افزایش نشان داد در حالی که در اکوتیپ دیابلورده تنها در تیمارهای بالای شوری افزایش یافت. میزان افزایش این پارامترها در دو اکوتیپ با یکدیگر متفاوت بود. این مشاهدات نشان می­ دهد که اکوتیپ یزدی از پاسخ­ های بیوشیمیایی و فیزیولوژیک با کارایی بالاتر برخوردار بود تا اثرات کاهشی شوری بر پارامترهای رشد را کم کند.  

Keywords [Persian]

  • بتاگلوکوزیداز
  • پلی فنل اکسیداز
  • محتوی فنل تام
  • ویژگی های روزنه ها
  • یونجه

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Journal of Plant Physiology and Breeding
Volume 10, Issue 2
December 2020
Pages 45-58

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